Osmyb3,SG3,SMG3

2015-01-20 | Categories genes  | Tags grain  grain length  breeding  transcriptional regulator  transcription activator  grain size  panicle  grains per panicle  grain number  nucleus  cell proliferation  grain weight  cell expansion  BR  BR signaling  BR  Ubiquitin 

• Information
  • Symbol: Osmyb3,SG3,SMG3
  • MSU: LOC_Os03g29614
  • RAPdb: Os03g0410000
• Publication
  • Cloning and expression of five myb-related genes from rice seed, 1997, Gene.
  • A minor QTL, SG3, encoding an R2R3-MYB protein, negatively controls grain length in rice., 2020, Theor Appl Genet..
  • Bin-based genome-wide association studies reveal superior alleles for improvement of appearance quality using a 4-way MAGIC population in rice, 2020, J Adv Res.
  • Identification of SMG3, a QTL Coordinately Controls Grain Size, Grain Number per Panicle, and Grain Weight in Rice., 2022, Front Plant Sci.
  • An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice., 2022, Plant Cell.
• Genbank accession number
  • D88619
• Key message
  • The genetic effect of SG3 on grain length was dependent on GS3 status
  • SG3 overexpression and knockdown plants showed shortened and elongated grains, respectively, which demonstrated that SG3 acts as a negative regulator of grain length
  • The isolation of the SG3 MYB gene provides new gene resource and contributes to the regulatory network of grain length in rice
  • SG3 and GS3 were frequently in coupling phase in indica rice, making them good targets for the breeding of cultivars with short or long grains
  • A gene encoding an R2R3 MYB domain transcriptional regulator was identified as the candidate gene for SG3
  • SG3 was preferentially expressed in panicles after flowering, and SG3 acted as a transcription activator
  • The SMG3 allele from M494 causes an increase in the number of grains per panicle but produces smaller grain size and thousand grain weight
  • Microscopy analysis shows that SMG3 mainly produces long grains by increasing in both cell length and cell number in the length direction, which thus enhances grain weight by promoting cell expansion and cell proliferation
  • Overexpression of SMG3 in rice produces a phenotype with more grains but reduces grain length and weight
  • Our results reveal that SMG3 plays an important role in the coordinated regulation of grain size, grain number per panicle, and grain weight, providing a new insight into synergistical modification on the grain appearance quality, grain number per panicle, and grain weight in rice
  • The SMG3 is constitutively expressed in various organs in rice, and the SMG3 protein is located in the nucleus
  • SMG3 interacts with another grain size regulator, DGS1
  • SMG3 and DGS1 are involved in BR signaling
  • SMG3 encodes a homolog of Arabidopsis (Arabidopsis thaliana) UBIQUITIN CONJUGATING ENZYME 32, which is a conserved ERAD-associated E2 ubiquitin conjugating enzyme
  • Further analyses showed that DGS1 is an active E3 ubiquitin ligase and colocates with SMG3 in the ER
• Connection
  • Osmyb3~SG3~SMG3, Osmyb5, Cloning and expression of five myb-related genes from rice seed, RT-PCR analysis showed that Osmyb2, Osmyb3 and Osmyb5 genes were expressed in all tissues examined
  • Osmyb3~SG3~SMG3, Osmyb5, Cloning and expression of five myb-related genes from rice seed, Another myb-like clone, Osmyb5, was obtained by screening a rice seed cDNA library with probes designed to recognize the AACA-like binding domain in GAMYB and PHMYB3
  • Osmyb3~SG3~SMG3, Osmyb5, Cloning and expression of five myb-related genes from rice seed, As Osmyb5 exhibits a high similarity to the regions in both GAMYB and PHMYB3, which can bind to the AACA motif, there is a possibility that the OSMYB5 protein may bind to the AACA motif of glutelin genes
  • Osmyb2, Osmyb3~SG3~SMG3, Cloning and expression of five myb-related genes from rice seed, RT-PCR analysis showed that Osmyb2, Osmyb3 and Osmyb5 genes were expressed in all tissues examined
  • GS3, Osmyb3~SG3~SMG3, A minor QTL, SG3, encoding an R2R3-MYB protein, negatively controls grain length in rice., In this study, we focused on the isolation of a minor grain length QTL, small grain 3 (SG3), which is closely linked to the major QTL grain size 3 (GS3)
  • GS3, Osmyb3~SG3~SMG3, A minor QTL, SG3, encoding an R2R3-MYB protein, negatively controls grain length in rice., The genetic effect of SG3 on grain length was dependent on GS3 status
  • GS3, Osmyb3~SG3~SMG3, A minor QTL, SG3, encoding an R2R3-MYB protein, negatively controls grain length in rice., A large number of near-isogenic F2 plants in which GS3 was fixed with nonfunctional alleles were developed to clone SG3
  • GS3, Osmyb3~SG3~SMG3, A minor QTL, SG3, encoding an R2R3-MYB protein, negatively controls grain length in rice., SG3 and GS3 were frequently in coupling phase in indica rice, making them good targets for the breeding of cultivars with short or long grains
  • Osmyb3~SG3~SMG3, OsRHC1~LE~TT3.1~DGS1, An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice., Here, we report that SMALL GRAIN 3 (SMG3) and DECREASED GRAIN SIZE 1 (DGS1), an ERAD-related E2-E3 enzyme pair, regulate grain size and weight through the brassinosteroid (BR) signaling pathway in rice (Oryza sativa)
  • Osmyb3~SG3~SMG3, OsRHC1~LE~TT3.1~DGS1, An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice., SMG3 interacts with another grain size regulator, DGS1
  • Osmyb3~SG3~SMG3, OsRHC1~LE~TT3.1~DGS1, An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice., Loss of function of SMG3 or DGS1 results in small grains, while overexpression of SMG3 or DGS1 leads to long grains
  • Osmyb3~SG3~SMG3, OsRHC1~LE~TT3.1~DGS1, An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice., Further analyses showed that DGS1 is an active E3 ubiquitin ligase and colocates with SMG3 in the ER
  • Osmyb3~SG3~SMG3, OsRHC1~LE~TT3.1~DGS1, An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice., SMG3 and DGS1 are involved in BR signaling
  • Osmyb3~SG3~SMG3, OsRHC1~LE~TT3.1~DGS1, An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice., Genetic analysis suggests that SMG3, DGS1, and BRI1 act together to regulate grain size and weight

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