- Information
- Symbol: OsHAK21,qSE3
- MSU: LOC_Os03g37930
- RAPdb: Os03g0576200
- Publication
- The potassium transporter OsHAK21 functions in the maintenance of ion homeostasis and tolerance to salt stress in rice., 2015, Plant Cell Environ.
- A quantitative trait locus, qSE3, promotes seed germination and seedling establishment under salinity stress in rice., 2018, Plant J.
- Identification and fine mapping of qGR6.2, a novel locus controlling rice seed germination under salt stress, 2021, BMC Plant Biol.
- Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., 2023, Front Plant Sci.
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Genbank accession number
- Key message
- Our analyses of subcellular localizations and expression patterns showed that OsHAK21 was localized in the plasma membrane, and expressed in xylem parenchyma and individual endodermal cells (putative passage cells)
- Here, we report a significant inducement of OsHAK21 expression by high salinity treatment, and provide genetic evidence of the involvement of OsHAK21 in rice salt tolerance
- The potassium transporter OsHAK21 functions in the maintenance of ion homeostasis and tolerance to salt stress in rice.
- Disruption of OsHAK21 rendered plants sensitive to salt stress
- These results demonstrate that OsHAK21 may mediate K(+) absorption by the plasma membrane, and play crucial roles in the maintenance of the Na(+) /K(+) homeostasis in rice under salt stress
- Further functional characterizations of OsHAK21 in K(+) uptake-deficient yeast and Arabidopsis revealed that OsHAK21 possesses K(+) transporter activity
- In this study, we mapped one major QTL qSE3 for seed germination and seedling establishment under salinity stress in rice
- All of these seed physiological changes modulated by qSE3 might contribute to seed germination and seedling establishment under salinity stress
- This study provides important insights into the roles of qSE3 in seed germination and seedling establishment under salinity stress and facilitates the practical use of qSE3 in rice breeding
- Based on analysis of single-nucleotide polymorphism data of rice accessions, we identified a HAP3 haplotype of qSE3 that was positively correlated with seed germination under salinity stress
- To understand the molecular basis of this QTL, we isolated qSE3 by map-based cloning and found that it encodes a K+ transporter gene, OsHAK21
- The expression of qSE3 was significantly up-regulated by salinity stress in germinating seeds
- Physiological analysis suggested that qSE3 significantly increased K+ and Na+ uptake in germinating seeds under salinity stress, resulting in increased abscisic acid (ABA) biosynthesis and activated ABA signaling responses
- Furthermore, qSE3 significantly decreased the H2 O2 level in germinating seeds under salinity stress
- Haplotype analysis revealed four key variations associated with salt tolerance, including an SNP (+1605 bp) within OsABCI7 exon, an SSR (-1231 bp) within OsHAK21 promoter, an indel site at OsNHX1 promoter (-822 bp), and an SNP (-1866 bp) within OsAKT2 promoter
- Connection
- OsCYB5-2, OsHAK21~qSE3, An endoplasmic reticulum-localized cytochrome b 5 regulates high-affinity K+ transport in response to salt stress in rice., In this study, we identified an endoplasmic reticulum-localized cytochrome b (5) (OsCYB5-2) that interacted with a high-affinity K(+) transporter (OsHAK21) at the plasma membrane
- OsCYB5-2, OsHAK21~qSE3, An endoplasmic reticulum-localized cytochrome b 5 regulates high-affinity K+ transport in response to salt stress in rice., The association of OsCYB5-2 with the OsHAK21 transporter caused an increase in transporter activity by enhancing the apparent affinity for K(+)-binding but not Na(+)-binding
- OsCYB5-2, OsHAK21~qSE3, An endoplasmic reticulum-localized cytochrome b 5 regulates high-affinity K+ transport in response to salt stress in rice., Heme binding to OsCYB5-2 was essential for the regulation of OsHAK21
- OsHAK21~qSE3, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
- OsHAK21~qSE3, OsHKT2;1~OsHKT1, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
- OsAKT2, OsHAK21~qSE3, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
- OsAKT2, OsHAK21~qSE3, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., Haplotype analysis revealed four key variations associated with salt tolerance, including an SNP (+1605 bp) within OsABCI7 exon, an SSR (-1231 bp) within OsHAK21 promoter, an indel site at OsNHX1 promoter (-822 bp), and an SNP (-1866 bp) within OsAKT2 promoter
- OsHAK21~qSE3, OsHCF222, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
- OsHAK21~qSE3, OsNHA1~OsSOS1, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
- OsHAK21~qSE3, OsNHX1, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
- OsHAK21~qSE3, OsNHX1, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., Haplotype analysis revealed four key variations associated with salt tolerance, including an SNP (+1605 bp) within OsABCI7 exon, an SSR (-1231 bp) within OsHAK21 promoter, an indel site at OsNHX1 promoter (-822 bp), and an SNP (-1866 bp) within OsAKT2 promoter
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