OsNCED3

2015-01-20 | Categories genes  | Tags ABA  seed  anther  drought tolerance  vascular bundle  leaf  leaf shape  seed germination  drought  leaf senescence  senescence  tolerance  abiotic stress  stress  biotic stress  ABA  quality  stress tolerance  plant growth  ABA biosynthesis  chilling  stress response  chilling stress  abscisic acid  signal transduction  resistance  defense  brown planthopper  breeding  resistant  growth  development  grain  grain size  dormancy  embryo  seed dormancy 

• Information
  • Symbol: OsNCED3
  • MSU: LOC_Os03g44380
  • RAPdb: Os03g0645900
• Publication
  • Ectopic expression of rice OsNCED3 in Arabidopsis increases ABA level and alters leaf morphology, 2010, Plant Science.
  • Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L, 2013, Planta.
  • ABA controls H2O2 accumulation through the induction of OsCATB in rice leaves under water stress, 2011, Plant Cell Physiol.
  • The rice ERF transcription factor OsERF922 negatively regulates resistance to Magnaporthe oryzae and salt tolerance, 2012, J Exp Bot.
  • A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice, 2012, J Exp Bot.
  • ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice, 2007, Plant Cell Physiol.
  • Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8’-hydroxylase in rice, 2007, Plant Cell Physiol.
  • Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis, 2009, Plant Cell Physiol.
  • 9-cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice, 2018, Frontiers in plant science.
  • The Wild Rice Locus CTS-12 Mediates ABA-Dependent Stomatal Opening Modulation to Limit Water Loss Under Severe Chilling Stress, 2020, Front Plant Sci.
  • SiMYB19 from Foxtail Millet Setaria italica Confers Transgenic Rice Tolerance to High Salt Stress in the Field., 2022, Int J Mol Sci.
  • Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens., 2022, BMC Genomics.
  • OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage., 2022, Int J Mol Sci.
  • ABA biosynthesis gene OsNCED3 contributes to preharvest sprouting resistance and grain development in rice., 2022, Plant Cell Environ.
• Genbank accession number
  • AY838899
• Key message
  • The data suggest that, in addition to ABA biosynthesis, the monocot OsNCED3 gene may have additional functions in shaping leaf morphology and vascular bundle development in Arabidopsis
  • Ectopic expression of rice OsNCED3 in Arabidopsis increases ABA level and alters leaf morphology
  • Furthermore, overexpression of OsNCED3 in wild-type Arabidopsis plants results in increased accumulation of ABA, reduced relative water loss, delayed seed germination, and greater drought tolerance relative to that of wild-type
  • As a key gene in ABA biosynthesis, OsNCED3 was significantly induced in rice by water stress treatment and such induction preceded the rapid increase in ABA
  • The differences in endogenous ABA levels in Doongara and R31 correlated with differences in expression of the ABA biosynthetic genes encoding zeaxanthin epoxidase (OSZEP1) and 9-cis-epoxycarotenoid dioxygenase (OSNCED2, OSNCED3) in anthers
  • Overexpression of OsFbx352 led to a reduction in expression of genes responsible for ABA synthesis (OsNced2, OsNced3) and an increase in expression of genes encoding ABA catabolism (OsAba-ox2, OsAba-ox3) in the presence of glucose
  • Transgenic Arabidopsis lines overexpressing the monocot OsNCED3 gene in a wild-type background result in a smaller and rounder leaf shape and midvein
  • Further analysis found that nced3 mutants contained lower ABA content compared with wild-type plants, overexpression of OsNCED3 in transgenic plants could enhance water stress tolerance, promote leaf senescence and increase ABA content
  • We conclude that OsNCED3 mediates seed dormancy, plant growth, abiotic stress tolerance, and leaf senescence by regulating ABA biosynthesis in rice; and may provide a new strategy for improving the quality of crop
  • Here, we report important functions of OsNCED3 in multi-abiotic stress tolerance in rice
  • The OsNCED3 is constitutively expressed in various tissues under normal condition, Its expression is highly induced by NaCl, PEG, and H2O2 stress, suggesting the roles for OsNCED3 in response to the multi-abiotic stress tolerance in rice
  • CRISPR/Cas9-edited OsNCED3 resulted in chilling sensitive of japonica rice ZH11, demonstrating the involvement of ABA pathway in chilling stress response
  • SiMYB19 overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene OsNCED3 and the ABA signal transduction pathway-related genes OsPK1 and OsABF2
  • This study shows that overexpression of OsNCED3 in rice improves resistance to BPH, and has potential merit in rice breeding programs
  • Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens.
  • In rice, OsNCED3 was shown to promote ABA synthesis, and improve abiotic stress tolerance, but the function of OsNCED3 in regulating rice defense against the brown planthopper (Nilaparvata lugens; BPH) has been unclear
  • Rice lines overexpressing OsNCED3 (OE) were more resistant to BPH than the wild-type cv
  • Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2
  • Disruption of OsNCED3 by CRISPR/Cas9-mediated mutagenesis led to a lower ABA and higher gibberellic acid (GA) levels (thus a skewed ABA/GA ratio) in the embryo, promoting embryos growth and breaking seed dormancy before seed maturity and harvest, thus decreased seed dormancy and enhanced PHS in rice
  • ABA biosynthesis gene OsNCED3 contributes to preharvest sprouting resistance and grain development in rice.
  • Intriguingly, the overexpression of OsNCED3 resulted in increased grain size and weight, whereas the disruption of OsNCED3 function decreased grain size and weight
  • However, the overexpression of OsNCED3 enhanced PHS resistance by regulating proper ABA/GA ratio in the embryo
  • Nucleotide diversity analyses suggested that OsNCED3 may be selected during japonica populations adaptation of seed dormancy and germination
  • OsNCED3 encodes a chloroplast-localized abscisic acid (ABA) biosynthetic enzyme highly expressed in the embryo of developing seeds
• Connection
  • OsFbx352, OsNCED3, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice, Overexpression of OsFbx352 led to a reduction in expression of genes responsible for ABA synthesis (OsNced2, OsNced3) and an increase in expression of genes encoding ABA catabolism (OsAba-ox2, OsAba-ox3) in the presence of glucose
  • OsNCED2, OsNCED3, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice, Overexpression of OsFbx352 led to a reduction in expression of genes responsible for ABA synthesis (OsNced2, OsNced3) and an increase in expression of genes encoding ABA catabolism (OsAba-ox2, OsAba-ox3) in the presence of glucose
  • OsNCED2, OsNCED3, ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice, The differences in endogenous ABA levels in Doongara and R31 correlated with differences in expression of the ABA biosynthetic genes encoding zeaxanthin epoxidase (OSZEP1) and 9-cis-epoxycarotenoid dioxygenase (OSNCED2, OSNCED3) in anthers
  • OsABA1~OSZEP1, OsNCED3, ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice, The differences in endogenous ABA levels in Doongara and R31 correlated with differences in expression of the ABA biosynthetic genes encoding zeaxanthin epoxidase (OSZEP1) and 9-cis-epoxycarotenoid dioxygenase (OSNCED2, OSNCED3) in anthers
  • OsNAC2~OsTIL1~OMTN2~OsORE1, OsNCED3, A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis., Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly upregulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as downregulating the ABA catabolic gene (OsABA8ox1)
  • OsNCED3, OsNCED4, Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 OsNCED4 in Arabidopsis confers sugar oversensitivity and drought tolerance., The native OsNCED3 and OsNCED4 promoters were expressed in an overlapping pattern in rice seeds and young seedlings, suggesting possible functional redundancy between OsNCED3 and OsNCED4
  • OsNCED3, OsNCED4, Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 OsNCED4 in Arabidopsis confers sugar oversensitivity and drought tolerance., At the one-leaf stage, similar regulation of OsNCED3 and OsNCED4 gene expression in roots or leaves in response to moderate salt stress (150<U+00A0>mM NaCl) was observed
  • OsNCED3, OsNCED4, Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 OsNCED4 in Arabidopsis confers sugar oversensitivity and drought tolerance., Like OsNCED3, OsNCED4 is functionally active in ABA biosynthesis in rice
  • OsNCED3, OsNCED4, Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 OsNCED4 in Arabidopsis confers sugar oversensitivity and drought tolerance., OsNCED3 and OsNCED4 might play redundant roles in controlling ABA biosynthesis in rice, as suggested by GUS staining assay, but this should be further analyzed through complementation of rice NCED knockout mutants
  • OsNAC2~OsTIL1~OMTN2~OsORE1, OsNCED3, Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways., Moreover, OsNAC2 directly interacted with the promoters of OsLEA3 and OsNCED3 in yeast one-hybrid assays
  • OsNAC2~OsTIL1~OMTN2~OsORE1, OsNCED3, OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, Moreover, OsNAC2 delays the germination of seeds and coleoptile growth through the ABA pathway instead of the ethylene and GA pathway, by targeting the promoters of OsNCED3, OsZEP1, and OsABA8ox1
  • OsAREB8~OsAREB1~OsbZIP46~OsABF2~ABL1, OsNCED3, SiMYB19 from Foxtail Millet Setaria italica Confers Transgenic Rice Tolerance to High Salt Stress in the Field., SiMYB19 overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene OsNCED3 and the ABA signal transduction pathway-related genes OsPK1 and OsABF2
  • OsNCED3, OsPK1, SiMYB19 from Foxtail Millet Setaria italica Confers Transgenic Rice Tolerance to High Salt Stress in the Field., SiMYB19 overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene OsNCED3 and the ABA signal transduction pathway-related genes OsPK1 and OsABF2
  • OsNCED3, SAPK10~OsSAPK10, miR2105 and the kinase OsSAPK10 co-regulate OsbZIP86 to mediate drought-induced ABA biosynthesis in rice., ) osa-miR2105 (miR2105) and the Stress/ABA-activated protein kinase (OsSAPK10) coordinately regulate the rice basic region-leucine zipper transcription factor (bZIP TF; OsbZIP86) at the posttranscriptional and posttranslational levels to control drought-induced ABA biosynthesis via modulation of rice 9-cis-epoxycarotenoid dioxygenase (OsNCED3) expression
  • OsNCED3, SAPK10~OsSAPK10, miR2105 and the kinase OsSAPK10 co-regulate OsbZIP86 to mediate drought-induced ABA biosynthesis in rice., OsSAPK10 can phosphorylate and activate OsbZIP86 to enhance the expression of OsNCED3
  • miR2105, OsNCED3, miR2105 and the kinase OsSAPK10 co-regulate OsbZIP86 to mediate drought-induced ABA biosynthesis in rice., ) osa-miR2105 (miR2105) and the Stress/ABA-activated protein kinase (OsSAPK10) coordinately regulate the rice basic region-leucine zipper transcription factor (bZIP TF; OsbZIP86) at the posttranscriptional and posttranslational levels to control drought-induced ABA biosynthesis via modulation of rice 9-cis-epoxycarotenoid dioxygenase (OsNCED3) expression
  • GORI~OsABT, OsNCED3, OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage., Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2
  • OsABA8ox2~OsCYP707A6, OsNCED3, OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage., Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2
  • OsNCED3, OsNCED4, OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage., Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2
  • OsLTPL23, OsNCED3, Rice lipid transfer protein, OsLTPL23, controls seed germination by regulating starch-sugar conversion and ABA homeostasis., Transcript profiles in the germinating seeds exhibited that the abscisic acid (ABA)-responsive genes, OsABI3 and OsABI5, and biosynthesis genes, OsNCED1, OsNCED2, OsNCED3 and OsNCED4, are obviously upregulated in the osltpl23 mutants compared to NIP plants, conversely, ABA metabolism genes OsABA8ox1, OsABA8ox2 and OsABA8ox3 are stepwise decreased

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