OsPUB24

• Symbol: OsPUB24
• MSU: LOC_Os03g45420
• RAPdb: Os03g0657100

• OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response., 2019, Plant J.

Genbank accession number

• The ospub24 T-DNA knock-out mutant and Ubi:RNAi-OsPUB24 knock-down rice plants displayed enhanced seedling growth, increased lamina joint bending, and hypersensitivity to brassinolide (BL)
• OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response.
• Here, we report that a rice U-box E3 ubiquitin (Ub) ligase OsPUB24 acts as a negative regulator in the BR signaling pathway via 26S proteasome-dependent degradation of OsBZR1
• Our findings suggest that OsPUB24 participates in OsBZR1 turnover and the regulatory networks of OsPUB24, OsSK22, and OsBZR1 are crucial for fine-tuning the BR response in rice
• In addition, the stability of OsPUB24 was downregulated by BL and bikinin, an inhibitor of OsSK22 (Oryza sativa Shaggy/GSK3-like kinase 22)
• OsSK22, the homolog of Arabidopsis BIN2 (BRASSINOSTEROID INSENSITIVE2) protein kinase, phosphorylated OsPUB24 and elevated the cellular stability of OsPUB24

• OsBZR1~BZR1, OsPUB24, OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response., OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response.
• OsBZR1~BZR1, OsPUB24, OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response., Here, we report that a rice U-box E3 ubiquitin (Ub) ligase OsPUB24 acts as a negative regulator in the BR signaling pathway via 26S proteasome-dependent degradation of OsBZR1
• OsBZR1~BZR1, OsPUB24, OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response., OsPUB24 ubiquitinated OsBZR1, resulting in the proteasomal degradation of OsBZR1
• OsBZR1~BZR1, OsPUB24, OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response., Our findings suggest that OsPUB24 participates in OsBZR1 turnover and the regulatory networks of OsPUB24, OsSK22, and OsBZR1 are crucial for fine-tuning the BR response in rice
• OsPUB24, OsPUB7, Knockout Mutants of OsPUB7 Generated Using CRISPR/Cas9 Revealed Abiotic Stress Tolerance in Rice., Protein-protein interaction analysis revealed that the expression of the genes (OsPUB23, OsPUB24, OsPUB66, and OsPUB67) known to be involved in stress increased in PUB7-GE and this, by forming a 1-node network with OsPUB66 and OsPUB7, acted as a negative regulator of drought and salinity stress