OsHOS1

• Symbol: OsHOS1
• MSU: LOC_Os03g52700
• RAPdb: Os03g0737200

• Isolation and characterization of rice Oryza sativa L. E3-ubiquitin ligase OsHOS1 gene in the modulation of cold stress response, 2013, Plant Mol Biol.
• The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors., 2015, Plant Physiol.
• Rice root curling, a response to mechanosensing, is modulated by the rice E3-ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 OsHOS1., 2016, Plant Signal Behav.

Genbank accession number

• To address this issue, we have identified a rice E3-ubiquitin ligase, OsHOS1, and characterized its role in the modulation of the cold stress response
• Taken together our results confirm the importance of OsHOS1, and thus of the proteasome, in the modulation of the cold stress signalling in rice
• Isolation and characterization of rice (Oryza sativa L.) E3-ubiquitin ligase OsHOS1 gene in the modulation of cold stress response
• The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors.
• The morphological analysis of the roots in transgenic RNAi::OsHOS1 (RNA interference) and wild type (WT) plants, exposed to a mechanical barrier, revealed that the OsHOS1 silencing plants keep a straight root in contrast to WT that exhibit root curling
• Using the yeast two-hybrid system and BiFC assays we showed that OsHOS1 interacts with two ERF transcription factors (TFs), OsEREBP1 and OsEREBP2, known to regulate OsRMC gene expression
• We showed that OsHOS1 is involved in the regulation of root curling after mechanosensing and that RNAi::OsHOS1 plants failed to exhibit the root curling phenotype observed in WT
• Altogether, our results highlight the role of the proteasome modulating plant responses to mechanical stimuli and suggest that OsHOS1 is a hub integrating environmental and hormonal signaling into plant growth and development

• OsEREBP1, OsHOS1, The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors., Using the yeast two-hybrid system and BiFC assays we showed that OsHOS1 interacts with two ERF transcription factors (TFs), OsEREBP1 and OsEREBP2, known to regulate OsRMC gene expression
• OsEREBP1, OsHOS1, The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors., In addition, we showed that OsHOS1 affects the stability of both TFs in a proteasome dependent way, suggesting that this E3-ubiquitin ligase targets OsEREBP1 and OsEREBP2 for degradation
• OsEREBP2~ERF99, OsHOS1, The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors., Using the yeast two-hybrid system and BiFC assays we showed that OsHOS1 interacts with two ERF transcription factors (TFs), OsEREBP1 and OsEREBP2, known to regulate OsRMC gene expression
• OsEREBP2~ERF99, OsHOS1, The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors., In addition, we showed that OsHOS1 affects the stability of both TFs in a proteasome dependent way, suggesting that this E3-ubiquitin ligase targets OsEREBP1 and OsEREBP2 for degradation
• OsHOS1, OsRMC~OsRLK, The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors., The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors.
• OsHOS1, OsRMC~OsRLK, The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors., Using the yeast two-hybrid system and BiFC assays we showed that OsHOS1 interacts with two ERF transcription factors (TFs), OsEREBP1 and OsEREBP2, known to regulate OsRMC gene expression
• OsHOS1, OsMAPK33~OsMAPK3, OsMAPK3 Phosphorylates OsbHLH002/OsICE1 and Inhibits Its Ubiquitination to Activate OsTPP1 and Enhances Rice Chilling Tolerance., OsMAPK3 interacts with OsbHLH002 to prevent its ubiquitination by the E3 ligase OsHOS1