- Information
- Symbol: OsIAA13,OsiIAA1
- MSU: LOC_Os03g53150
- RAPdb: Os03g0742900
- Publication
- Exogenous auxin enhances the degradation of a light down-regulated and nuclear-localized OsiIAA1, an Aux/IAA protein from rice, via proteasome, 2005, Biochim Biophys Acta.
- OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, 2012, Plant Sci.
- A comprehensive transcriptome analysis of contrasting rice cultivars highlights the role of auxin and ABA responsive genes in heat stress response, 2021, Genomics.
- Identification of the OsCML4 Gene in Rice Related to Salt Stress Using QTL Analysis., 2022, Plants (Basel).
- Genbank accession number
- Key message
- Earlier, we reported the isolation of OsiIAA1 cDNA, first monocot member of Aux/IAA gene family from rice
- Extending this work further, we have isolated the OsiIAA1 gene from rice localized on chromosome 3
- The increased accumulation of OsiIAA1 transcript in auxin-treated rice coleoptiles even in the presence of a protein synthesis inhibitor, cycloheximide, suggested that OsiIAA1 is a primary auxin response gene; the expression of OsiIAA1 gene was also upregulated in the presence of cycloheximide alone
- The OsiIAA1 transcript levels were down-regulated in etiolated rice coleoptiles irradiated with far-red, red and blue light, suggesting the existence of a cross-talk between auxin and light signaling
- The antibodies raised against His6-OsiIAA1 recombinant protein could detect the OsiIAA1 protein in the plant extract only in the presence of a proteasome inhibitor, MG132, indicating that OsiIAA1 is rapidly degraded by proteasome complex
- Also, the proteasome inhibitor MG132 stabilized the purified His6-OsiIAA1 protein to some extent in the cell-free extracts of rice coleoptiles
- The OsiIAA1 protein harbors two nuclear localization signals (NLSs), one bipartite and the other resembling SV40 type NLS
- These studies indicate that nuclear localization of OsiIAA1 could be a prerequisite for its role in auxin signal transduction
- Exogenous auxin enhances the degradation of a light down-regulated and nuclear-localized OsiIAA1, an Aux/IAA protein from rice, via proteasome
- In this study, we newly isolated and characterized a rice gain-of-function mutant, Osiaa13, containing a single amino acid substitution in the core sequence required for the degradation of the OsIAA13 protein
- The Osiaa13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective
- Osiaa13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites
- Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in Osiaa13
- Taken together, our results indicate that OsIAA13 is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in rice
- OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice
- Connection
- OsDi19-1, OsIAA13~OsiIAA1, A comprehensive transcriptome analysis of contrasting rice cultivars highlights the role of auxin and ABA responsive genes in heat stress response, The changes in expression of few auxin and ABA associated genes, such as OsIAA13, OsIAA20, ILL8, OsbZIP12, OsPP2C51, OsDi19-1 and OsHOX24, among others, were validated under high-temperature conditions using RT-qPCR
- OsHOX24, OsIAA13~OsiIAA1, A comprehensive transcriptome analysis of contrasting rice cultivars highlights the role of auxin and ABA responsive genes in heat stress response, The changes in expression of few auxin and ABA associated genes, such as OsIAA13, OsIAA20, ILL8, OsbZIP12, OsPP2C51, OsDi19-1 and OsHOX24, among others, were validated under high-temperature conditions using RT-qPCR
- OsIAA13~OsiIAA1, OsPP2C51, A comprehensive transcriptome analysis of contrasting rice cultivars highlights the role of auxin and ABA responsive genes in heat stress response, The changes in expression of few auxin and ABA associated genes, such as OsIAA13, OsIAA20, ILL8, OsbZIP12, OsPP2C51, OsDi19-1 and OsHOX24, among others, were validated under high-temperature conditions using RT-qPCR
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