OsSPO11-1,OsTOP6A1

2015-01-20 | Categories genes  | Tags growth  vegetative  meiotic  flower  meiosis  abiotic stress 

• Information
  • Symbol: OsSPO11-1,OsTOP6A1
  • MSU: LOC_Os03g54091
  • RAPdb: Os03g0752200
• Publication
  • The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, 2012, Plant J.
  • Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, 2006, FEBS J.
  • Constitutive expression of a meiotic recombination protein gene homolog, OsTOP6A1, from rice confers abiotic stress tolerance in transgenic Arabidopsis plants, 2008, Plant Cell Rep.
  • OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice, 2010, Chromosoma.
• Genbank accession number
  • AJ549926
  • GU170363
  • AJ549926
  • GU170363
• Key message
  • Rice plants with loss-of-function of OsSPO11-1 have no apparent growth defects during vegetative development, but homologous chromosome pairing and recombination are significantly obstructed
  • Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • The over expression of OsTOP6A1 in transgenic Arabidopsis plants driven by cauliflower mosaic virus-35S promoter resulted in pleiotropic effects on plant growth and development
  • OsTOP6A1 was predominantly expressed in prepollinated flowers
  • Although AtSPO11-1, the homolog of OsTOP6A1 in Arabidopsis, has been implicated in meiotic recombination; the present study demonstrates possible additional role of OsTOP6A1 and provides an effective tool for engineering crop plants for tolerance to different environmental stresses
  • Constitutive expression of a meiotic recombination protein gene homolog, OsTOP6A1, from rice confers abiotic stress tolerance in transgenic Arabidopsis plants
  • Thus, OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice
• Connection
  • MER3~RCK, OsSPO11-1~OsTOP6A1, OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice, The crossover protein, MER3, isn’t efficiently assembled onto chromosomes and the lack of bivalent suggests that crossovers are also affected in the absence of OsSPO11-1
  • OsCOM1, OsSPO11-1~OsTOP6A1, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • MER3~RCK, OsSPO11-1~OsTOP6A1, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsRad21-4~OsREC8, OsSPO11-1~OsTOP6A1, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsSPO11-1~OsTOP6A1, ZEP1~OsZEP, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsSPO11-1~OsTOP6A1, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsSPO11-1~OsTOP6A1, OsTOP6B, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, Yeast two-hybrid analysis revealed that the full-length OsTOP6B protein interacts with both OsTOP6A2 and OsTOP6A3, but not with OsTOP6A1
  • OsSPO11-1~OsTOP6A1, OsTOP6A3, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, Yeast two-hybrid analysis revealed that the full-length OsTOP6B protein interacts with both OsTOP6A2 and OsTOP6A3, but not with OsTOP6A1
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, Yeast two-hybrid analysis revealed that the full-length OsTOP6B protein interacts with both OsTOP6A2 and OsTOP6A3, but not with OsTOP6A1
  • OsMTOPVIB, OsSPO11-1~OsTOP6A1, OsMTOPVIB is required for meiotic bipolar spindle assembly., We further revealed that the mono-orientation of sister kinetochores was prevalent in OsmtopVIB, whereas biorientation of sister kinetochores was frequently observed in Osspo11-1, pair2, and crc1 In addition, mutations of the cohesion subunit OsREC8 resulted in biorientation of sister kinetochores as well as bipolar spindles even in the background of OsmtopVIB Therefore, we propose that biorientation of the kinetochore is required for bipolar spindle assembly in the absence of homologous recombination
  • OsATM, OsSPO11-1~OsTOP6A1, OsATM safeguards accurate repair of meiotic double-strand breaks in rice., Aberrant chromosome associations disappeared in Osspo11-1 Osatm-1 double mutants and more severe defects were observed in Osdmc1 Osatm, suggesting that OsATM functions downstream of OsSPO11-1-catalyzed double-strand break (DSB) formation and in parallel with OsDMC1-mediated homologous recombination
  • OsSPO11-1~OsTOP6A1, OsSUN1, The SUN domain proteins OsSUN1 and OsSUN2 play critical but partially redundant roles in meiosis, When introducing a mutant allele of OsSPORULATION11 (OsSPO11)-1, which encodes a topoisomerase initiating homologous recombination, into the Ossun1 Ossun2 mutant, we observed a combined Osspo11-1- and Ossun1 Ossun2-like phenotype, demonstrating that OsSUN1 and OsSUN2 promote bouquet formation independent of OsSPO11-1, but regulate pairing and crossover formation downstream of OsSPO11-1
  • OsSPO11-1~OsTOP6A1, OsSUN2, The SUN domain proteins OsSUN1 and OsSUN2 play critical but partially redundant roles in meiosis, When introducing a mutant allele of OsSPORULATION11 (OsSPO11)-1, which encodes a topoisomerase initiating homologous recombination, into the Ossun1 Ossun2 mutant, we observed a combined Osspo11-1- and Ossun1 Ossun2-like phenotype, demonstrating that OsSUN1 and OsSUN2 promote bouquet formation independent of OsSPO11-1, but regulate pairing and crossover formation downstream of OsSPO11-1
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, To clarify the roles of these SPO11 paralogs, we used CRISPR/Cas9 mutagenesis to induce null mutants in OsSPO11-1, OsSPO11-2 and OsSPO11-4
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Similar to Osspo11-1, biallelic mutations in OsSPO11-2 first exon led to complete panicle sterility, and consequently, none of these primary transformants produced seeds
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Similar to Osspo11-1, the pollen mother cells of Osspo11-2 T1 progeny plants, showed an absence of bivalent formation at metaphase I, aberrant segregation of homologous chromosomes and formation of nonviable tetrads
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Altogether, this study demonstrates that whereas OsSPO11-1 and OsSPO11-2 are the likely orthologs of Arabidopsis AtSPO11-1 and AtSPO11-2, the additional OsSPO11-4 gene has no major role in wild-type rice meiosis

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