- Information
- Symbol: OsTPKa
- MSU: LOC_Os03g54100
- RAPdb: Os03g0752300
- Publication
- Genbank accession number
- Key message
- On the other hand, positively transformed T(0) plants with the OsTPKa gene from Horkuch consistently showed growth advantage under both control and salt stress
- DISCUSSION: The poor performance of the transgenic plants with the down-regulated genes OsTPKa and OsHAK_like under salt stress supports the assumption that OsTPKa and OsHAK_like play important roles in defending the rice landrace Horkuch against salt stress, minimizing salt injury, and maintaining plant growth
- Moreover, the growth advantage provided by overexpression of the vacuolar OsTPKa K(+) transporter, particularly under salt stress reconfirms its important role in providing salt tolerance
- These are OsTPKa or Vacuolar two-pore potassium channel and OsHAK_like or a hypothetical protein of the HAK family
- Connection
- OsTPKa, OsTPKb, Rice two-pore K+ channels are expressed in different types of vacuoles, The Oryza sativa genome encodes two TPK isoforms (TPKa and TPKb) that have very similar sequences and are ubiquitously expressed
- OsTPKa, OsTPKb, Rice two-pore K+ channels are expressed in different types of vacuoles, In spite of high levels of similarity in sequence and transport properties, the cellular localization of TPKa and TPKb channels was different, with TPKa localization predominantly at the large LV and TPKb primarily in smaller PSV-type compartments
- OsTPKa, OsTPKb, Rice two-pore K+ channels are expressed in different types of vacuoles, Trafficking of TPKa was sensitive to brefeldin A, while that of TPKb was not
- OsTPKa, OsTPKb, Rice two-pore K+ channels are expressed in different types of vacuoles, The use of TPKa:TPKb chimeras showed that C-terminal domains are crucial for the differential targeting of TPKa and TPKb
- OsTPKa, OsTPKb, Rice two-pore K+ channels are expressed in different types of vacuoles, Site-directed mutagenesis of C-terminal residues that were different between TPKa and TPKb identified three amino acids that are important in determining ultimate vacuolar destination
- OsHAK_like, OsTPKa, Functional genomic analysis of K+ related salt-responsive transporters in tolerant and sensitive genotypes of rice., These are OsTPKa or Vacuolar two-pore potassium channel and OsHAK_like or a hypothetical protein of the HAK family
- OsHAK_like, OsTPKa, Functional genomic analysis of K+ related salt-responsive transporters in tolerant and sensitive genotypes of rice., For further insight, OsTPKa and OsHAK_like were chosen for loss-of-function genomic analysis in Horkuch using the CRISPR/Cas9 tool
- OsHAK_like, OsTPKa, Functional genomic analysis of K+ related salt-responsive transporters in tolerant and sensitive genotypes of rice., RESULTS: The promoter sequences of the OsTPKa and OsHAK_like genes showed some significant differences in promoter sequences which may give a survival advantage to Horkuch under salt-stress
- OsHAK_like, OsTPKa, Functional genomic analysis of K+ related salt-responsive transporters in tolerant and sensitive genotypes of rice., DISCUSSION: The poor performance of the transgenic plants with the down-regulated genes OsTPKa and OsHAK_like under salt stress supports the assumption that OsTPKa and OsHAK_like play important roles in defending the rice landrace Horkuch against salt stress, minimizing salt injury, and maintaining plant growth
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