- Information
- Symbol: Pho1
- MSU: LOC_Os03g55090
- RAPdb: Os03g0758100
- Publication
- Mutation of the plastidial alpha-glucan phosphorylase gene in rice affects the synthesis and structure of starch in the endosperm, 2008, Plant Cell.
- Rice endosperm starch phosphorylase Pho1 assembles with disproportionating enzyme Dpe1 to form a protein complex that enhances synthesis of malto-oligosaccharides., 2016, J Biol Chem.
- Modulation of Shoot Phosphate Level and Growth by PHOSPHATE1 Upstream Open Reading Frame , 2020, Plant Physiol.
- Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm., 2023, Theor Appl Genet.
- Genbank accession number
- Key message
- The loss of Pho1 caused smaller starch granules to accumulate and modified the amylopectin structure
- These results strongly suggest that Pho1 plays a crucial role in starch biosynthesis in rice endosperm at low temperatures and that one or more other factors can complement the function of Pho1 at high temperatures
- The phenotype of the pho1 mutation was temperature dependent
- Plastidial phosphorylase (Pho1) accounts for approximately 96% of the total phosphorylase activity in developing rice (Oryza sativa) seeds
- Variation in the morphological and biochemical phenotype of individual seeds was common to all 15 pho1-independent homozygous mutant lines studied, indicating that this phenotype was caused solely by the genetic defect
- Our work suggests that regulation of PHO1 expression via its uORF might be a genetic resource useful-both in natural populations and in the context of genome editing-toward improving plant growth under Pi-deficient conditions
- We further show that natural accessions lacking the PHO1 uORF exhibit higher PHO1 protein levels and shoot Pi content
- Increased shoot Pi content was linked to the absence of the PHO1 uORF in a population of F2 segregants
- In this work, we demonstrate that an upstream open reading frame (uORF) present in the 5’ untranslated region of the Arabidopsis (Arabidopsis thaliana) PHO1 inhibits its translation and influences Pi homeostasis
- A point mutation removing the PHO1 uORF (
uORF) in transgenic Arabidopsis resulted in increased association of its mRNA with polysomes and led to higher PHO1 protein levels, independent of Pi availability - Pho1 deficiency impaired MOS mobilization, triggering short MOS accumulation and starch synthesis reduction during early seed development
- Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm.
- These findings show that Pho1 cooperates with DPE1 to control short MOS mobilization during starch synthesis initiation in rice endosperm
- Connection
- DPE1, Pho1, Rice endosperm starch phosphorylase Pho1 assembles with disproportionating enzyme Dpe1 to form a protein complex that enhances synthesis of malto-oligosaccharides., Rice endosperm starch phosphorylase (Pho1) assembles with disproportionating enzyme (Dpe1) to form a protein complex that enhances synthesis of malto-oligosaccharides.
- OsPHO1;2, Pho1, Node-localized transporters of phosphorus essential for seed development in rice., Here, we found that two members (OsPHO1;1 and OsPHO1;2) belonging to PHO1 gene family, are involved in the distribution of P to the seeds in rice
- OsPHO1;1, Pho1, Node-localized transporters of phosphorus essential for seed development in rice., Here, we found that two members (OsPHO1;1 and OsPHO1;2) belonging to PHO1 gene family, are involved in the distribution of P to the seeds in rice
- DPE1, Pho1, Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm., Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm.
- DPE1, Pho1, Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm., By mutant analyses and biochemical investigations, we present here functional identifications of plastidial α-glucan phosphorylase (Pho1) and disproportionating enzyme (DPE1) during starch synthesis initiation in rice (Oryza sativa) endosperm
- DPE1, Pho1, Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm., Overexpression of DPE1 in pho1 resulted in plump seeds only
- DPE1, Pho1, Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm., Knockout of DPE1 in pho1 completely blocked MOS mobilization, resulting in severely and excessively Shr seeds only
- DPE1, Pho1, Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm., These findings show that Pho1 cooperates with DPE1 to control short MOS mobilization during starch synthesis initiation in rice endosperm
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