OsNAC19,SNAC1,OsNAC9

2015-01-20 | Categories genes  | Tags salt  ABA  sheath  oxidative  root architecture  stomatal  drought  ethylene  root  yield  salinity  grain  architecture  drought resistance  jasmonate  blast  seed  culm  seedling  transcription factor  reproductive  grain yield  stomata  resistance  stress  ABA  drought stress  drought stress 

• Information
  • Symbol: OsNAC19,SNAC1,OsNAC9
  • MSU: LOC_Os03g60080
  • RAPdb: Os03g0815100
• Publication
  • Rice gene OsNAC19 encodes a novel NAC-domain transcription factor and responds to infection by Magnaporthe grisea, 2007, Plant Science.
  • The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, 2010, Mol Genet Genomics.
  • The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice, 2013, J Exp Bot.
  • The overexpression of OsNAC9 alters the root architecture of rice plants enhancing drought resistance and grain yield under field conditions, 2012, Plant Biotechnol J.
  • Overexpressing a NAM, ATAF, and CUC NAC transcription factor enhances drought resistance and salt tolerance in rice, 2006, Proc Natl Acad Sci U S A.
  • A homolog of human ski-interacting protein in rice positively regulates cell viability and stress tolerance, 2009, Proc Natl Acad Sci U S A.
  • Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, 2014, Plant Cell Rep.
  • Genome-Wide Identification of SNAC1-Targeted Genes Involved in Drought Response in Rice., 2019, Front Plant Sci.
  • Transcription factor OsSNAC1 positively regulates nitrate transporter gene expression in rice., 2023, Plant Physiol.
• Genbank accession number
  • AK067690
  • DQ394702
  • AK067690
  • DQ394702
• Key message
  • Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • The expression of OsNAC19 in rice leaves could be induced by the infection of blast fungus, and by application of exogenous methyl jasmonate (MeJA), ABA and ethylene but ethylene had a relatively weak induction effect
  • The expression of gene OsNAC19 was high in rice seedling roots, culms and blade sheathes, but its expression in rice leaves was low
  • Here, a rice homologue of SRO (similar to RCD one), termed OsSRO1c, was identified as a direct target gene of SNAC1 (stress-responsive NAC 1) involved in the regulation of stomatal aperture and oxidative response
  • The results suggest that OsSRO1c has dual roles in drought and oxidative stress tolerance of rice by promoting stomatal closure and H(2)O(2) accumulation through a novel pathway involving regulators SNAC1 and DST
  • The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice
  • Such up-regulated genes that are commonly and specifically up-regulated in OsNAC9 transgenic roots may account for the altered root architecture conferring increased drought resistance phenotype
  • The overexpression of OsNAC9 alters the root architecture of rice plants enhancing drought resistance and grain yield under field conditions
  • Under drought conditions, RCc3:OsNAC9 plants showed an increased grain yield of 28%-72%, whilst the GOS2:OsNAC9 plants remained unchanged
  • The aerenchyma of RCc3:OsNAC9 roots was enlarged to a greater extent than those of GOS2:OsNAC9 and non-transgenic (NT) roots, suggesting the importance of this phenotype for enhanced drought resistance
  • Field evaluations over two cultivating seasons showed that grain yields of the RCc3:OsNAC9 and the GOS2:OsNAC9 plants were increased by 13%-18% and 13%-32% under normal conditions, respectively
  • Here, we show that overexpression of stress responsive gene SNAC1 (STRESS-RESPONSIVE NAC 1) significantly enhances drought resistance in transgenic rice (22-34% higher seed setting than control) in the field under severe drought stress conditions at the reproductive stage while showing no phenotypic changes or yield penalty
  • The OsSKIPa-overexpressing rice showed significantly increased reactive oxygen species-scavenging ability and transcript levels of many stress-related genes, including SNAC1 and rice homologs of CBF2, PP2C, and RD22, under drought stress conditions
  • Our data suggest that SNAC1 holds promising utility in improving drought and salinity tolerance in rice
  • In this research, a full-length cDNA clone OsNAC19 (Oryza sativa NAC19), encoding a novel NAC-domain protein, was isolated from a cDNA library prepared with rice leaves infected by incompatible race 131 of blast fungus (Magnaporthe grisea)
  • SNAC1 is induced predominantly in guard cells by drought and encodes a NAM, ATAF, and CUC (NAC) transcription factor with transactivation activity
  • Root-specific (RCc3) and constitutive (GOS2) promoters were used to overexpress OsNAC9 and produced the transgenic RCc3:OsNAC9 and GOS2:OsNAC9 plants
  • Interestingly, O-methyltransferase, a gene necessary for barrier formation, was specifically up-regulated only in the RCc3:OsNAC9 roots
  • Rice gene OsNAC19 encodes a novel NAC-domain transcription factor and responds to infection by Magnaporthe grisea
  • A stress-responsive NAC transcription factor gene SNAC1 has been reported for its function in the positive regulation of drought resistance in rice, and several downstream SNAC1 targets have been identified
  • However, a complete regulatory network mediated by SNAC1 in drought response remains unknown
  • In this study, we performed Chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq of SNAC1-overexpression transgenic rice (SNAC1-OE) lines and wild-type under normal and moderate drought stress conditions, to identify all SNAC1 target genes at a genome-wide scale by RNA-Seq analyses
  • SNAC1 can bind to the OsbZIP23 promoter, an important ABA signaling regulator, and positively regulate the expression of several ABA signaling genes
• Connection
  • OsNAC19~SNAC1~OsNAC9, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Pull-down assays revealed that OsNAC5 interacts with OsNAC5, OsNAC6 and SNAC1
  • OsNAC19~SNAC1~OsNAC9, OsNAC5, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Pull-down assays revealed that OsNAC5 interacts with OsNAC5, OsNAC6 and SNAC1
  • OsNAC19~SNAC1~OsNAC9, OsNCED4, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsbZIP23, OsNAC19~SNAC1~OsNAC9, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsLEA3~OsLEA3-1, OsNAC19~SNAC1~OsNAC9, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsNAC19~SNAC1~OsNAC9, OsP5CS~OsP5CS1, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsDSR2, OsNAC19~SNAC1~OsNAC9, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • DST~WL1~HST1, OsNAC19~SNAC1~OsNAC9, The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice, The results suggest that OsSRO1c has dual roles in drought and oxidative stress tolerance of rice by promoting stomatal closure and H(2)O(2) accumulation through a novel pathway involving regulators SNAC1 and DST
  • OsNAC19~SNAC1~OsNAC9, OsSRO1c~BOC1, The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice, Here, a rice homologue of SRO (similar to RCD one), termed OsSRO1c, was identified as a direct target gene of SNAC1 (stress-responsive NAC 1) involved in the regulation of stomatal aperture and oxidative response
  • OsNAC19~SNAC1~OsNAC9, OsSRO1c~BOC1, The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice, SNAC1 could bind to the promoter of OsSRO1c and activate the expression of OsSRO1c
  • OsNAC19~SNAC1~OsNAC9, OsSRO1c~BOC1, The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice, The results suggest that OsSRO1c has dual roles in drought and oxidative stress tolerance of rice by promoting stomatal closure and H(2)O(2) accumulation through a novel pathway involving regulators SNAC1 and DST
  • OsNAC19~SNAC1~OsNAC9, OsSRO1c~BOC1, The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice, The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice
  • OsNAC19~SNAC1~OsNAC9, OsSKIPa, A homolog of human ski-interacting protein in rice positively regulates cell viability and stress tolerance, The OsSKIPa-overexpressing rice showed significantly increased reactive oxygen species-scavenging ability and transcript levels of many stress-related genes, including SNAC1 and rice homologs of CBF2, PP2C, and RD22, under drought stress conditions
  • OsNAC19~SNAC1~OsNAC9, OsPP18~OsPP2C10, A SNAC1-regulated protein phosphatase gene OsPP18 modulates drought and oxidative stress tolerance through ABA-independent reactive oxygen species scavenging in rice, We identified a rice protein phosphatase 2C (PP2C) gene, OsPP18, as a STRESS-RESPOSIVE NAC1 (SNAC1)-regulated downstream gene.
  • OsNAC19~SNAC1~OsNAC9, OsSIDP366, OsSIDP366, a DUF1644 gene, positively regulates responses to drought and salt stresses in rice, Digital gene expression (DGE) profile analysis indicated that stress related genes such as SNAC1, OsHAK5 and PRs were up-regulated in OsSIDP366-overexpressing plants.
  • OsbZIP23, OsNAC19~SNAC1~OsNAC9, Genome-Wide Identification of SNAC1-Targeted Genes Involved in Drought Response in Rice., SNAC1 can bind to the OsbZIP23 promoter, an important ABA signaling regulator, and positively regulate the expression of several ABA signaling genes
  • OsBI-1, OsNAC19~SNAC1~OsNAC9, Profiling of rice Cd-tolerant genes through yeast-based cDNA library survival screening, Among the Cd-tolerant genes identified, several categories of genes such as BAX inhibitor (BI), NAC transcription factors and Rapid ALkalinization Factors (RALFs) were of particular interest, and their function of Cd tolerance was further validated via heterologous expression, which suggested that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves
  • OsNAC19~SNAC1~OsNAC9, RALF12, Profiling of rice Cd-tolerant genes through yeast-based cDNA library survival screening, Among the Cd-tolerant genes identified, several categories of genes such as BAX inhibitor (BI), NAC transcription factors and Rapid ALkalinization Factors (RALFs) were of particular interest, and their function of Cd tolerance was further validated via heterologous expression, which suggested that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves
  • OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., IPA1 improves drought tolerance by activating SNAC1 in rice.
  • OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., Yeast one-hybrid, dual-luciferase and electrophoretic mobility shift assays indicated that the IPA1 directly activates the promoter of SNAC1
  • OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., Expression of SNAC1 is significantly down-regulated in IPA1 knockout plants
  • OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice

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