OsPHY2

2015-01-20 | Categories genes  | Tags temperature  growth  seed  seed germination  cytoplasm  primary root  phosphate  phosphorus 

• Information
  • Symbol: OsPHY2
  • MSU: LOC_Os03g60370
  • RAPdb: Os03g0818100
• Publication
  • Molecular characterization and functional analysis of OsPHY2, a phytase gene classified in histidine acid phosphatase type in rice Oryza sativa L., 2011, African Journal of Biotechnology.
• Genbank accession number
  • NM_001058240
• Key message
  • Prokaryotic expressed OsPHY2 has a strong function on degradation of phytate, with optimal temperature of 47°C and optimal pH of 3
  • Under the guidance of patatin signal peptide (PSP) of potato, the OsPHY2 expressed in tobacco could be secreted by the rhizosphere and hydrolyze the phytate into Pi, leading to a significantly more inorganic phosphate (Pi) accumulated and growth improved in plants under the condition using phytate as the sole phosphorus source
  • During the seed germination, the transcripts of OsPHY2 were much more detected in the germinated seeds than in the primary roots and the leaves
  • Therefore, as one HAP type of the phytase genes, OsPHY2 plays a critical role on the degradation of the phytins during the seed germination and has a potential application on the generation of elite crop germplasms with high use of efficiency of phytate and a large quantity of organic phosphate compounds in the arable land
  • OsPHY2 contains HP_HAP_like domain, a 24-aa signal peptide at the N-terminal and was targeted to the cytoplasm membrane after ER sorted
• Connection

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