OsACOS12

• Symbol: OsACOS12
• MSU: LOC_Os04g24530
• RAPdb: Os04g0310800

• OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice., 2016, BMC Plant Biol.
• Rice fatty acyl-CoA synthetase OsACOS12 is required for tapetum programmed cell death and male fertility., 2017, Planta.
• Acyl-CoA synthetases from Physcomitrella, rice and Arabidopsis: different substrate preferences but common regulation by MS188 in sporopollenin synthesis., 2019, Planta.
• OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice, 2021, Rice (N Y).

Genbank accession number

• ACOS5 and OsACOS12 are conserved for pollen wall formation in monocot and dicot species
• The localization of OsACOS12-GFP demonstrated that OsACOS12 protein is accumulated in tapetal cells and anther locules
• The osacos12 mutation caused by a pre-mature stop codon in LOC_Os04g24530 exhibits defective sexine resulting in a male sterile phenotype in rice
• OsACOS12 driven by the ACOS5 promoter could partially restore the male fertility of the acos5 mutant in Arabidopsis
• In situ hybridization shows that OsACOS12 is expressed in tapetal cells and microspores at the transcript level
• Loss of function mutation of rice OsACOS12 impairs lipid metabolism-mediated anther cuticle and pollen wall formation, and interferes with tapetum programmed cell death, leading to male sterility
• Rice fatty acyl-CoA synthetase OsACOS12 is required for tapetum programmed cell death and male fertility.
• Those phenotypic changes were evident at stage 10, during which OsACOS12 had its maximum expression in tapetal cells and microspores
• A complementation test showed that OsACOS12 driven by the ACOS5 promoter could partially restore the male fertility of the acos5 mutant in Arabidopsis, while PpACOS6 did not rescue the acos5 phenotype

• OsACOS12, STS1~OsLDDT1~OsSTS1, SWOLLEN TAPETUM AND STERILITY 1 Is Required for Tapetum Degeneration and Pollen Wall Formation in Rice., Moreover, STS1 interacts with Polyketide Synthase 2 (OsPKS2) and Acyl-CoA Synthetase 12 (OsACOS12), two enzymes crucial in lipidic sporopollenin biosynthesis in pollen wall formation, suggesting a potentially lipidic metabolon for sporopollenin biosynthesis in rice
• OsACOS12, OsTKPR2, OsTKPR2 is part of a sporopollenin-producing metabolon required for exine formation in rice., OsTKPR1 and OsTKPR2 were integrated into a metabolon including OsACOS12 and OsPKS2, which resulted in a significant increased enzymatic efficiency when both OsTKPR1 and OsTKPR2 were present, indicating the mutual dependence of OsTKPR2 and OsTKPR1 for their full biochemical activities