GIF1,OsCIN2,OsGIF1,WB1

2015-01-20 | Categories genes  | Tags domestication  grain  homeostasis  grain weight  cell wall  development  grain size  vegetative  reproductive  breeding  organ size  reproductive development  starch  endosperm  chalkiness  endosperm development 

• Information
  • Symbol: GIF1,OsCIN2,OsGIF1,WB1
  • MSU: LOC_Os04g33740
  • RAPdb: Os04g0413500
• Publication
  • Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, 2010, BMC Evol Biol.
  • Sugar homeostasis mediated by cell wall invertase GRAIN INCOMPLETE FILLING 1 GIF1 plays a role in pre-existing and induced defence in rice, 2014, Mol Plant Pathol.
  • Control of rice grain-filling and yield by a gene with a potential signature of domestication, 2008, Nat Genet.
  • OsGIF1 Positively Regulates the Sizes of Stems, Leaves, and Grains in Rice., 2017, Front Plant Sci.
  • WB1, a Regulator of Endosperm Development in Rice, Is Identified by a Modified MutMap Method., 2018, Int J Mol Sci.
• Genbank accession number
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
  • EU095553
  • GU797931
  • GU797932
  • GU797933
  • GU797934
  • GU797936
  • GU797937
  • GU797938
  • GU797942
  • GU797945
  • GU797947
  • GU797948
  • GU797949
• Key message
  • We previously demonstrated that the rice cell-wall invertase (CWI) gene GIF1 that plays an important role in the grain-filling process was most likely subjected to domestication selection in the promoter region
  • Results based on analyses of population genetics and gene phylogenetic tree of 25 cultivars and 25 wild rice sequences demonstrated that OsCIN1 was also artificially selected during rice domestication with a fixed mutation in the coding region, in contrast to GIF1 that was selected in the promoter region
  • Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication
  • Here we report the isolation and functional analysis of the rice GIF1 (GRAIN INCOMPLETE FILLING 1) gene that encodes a cell-wall invertase required for carbon partitioning during early grain-filling
  • The cultivated GIF1 gene shows a restricted expression pattern during grain-filling compared to the wild rice allele, probably a result of accumulated mutations in the gene’s regulatory sequence through domestication
  • Fine mapping with introgression lines revealed that the wild rice GIF1 is responsible for grain weight reduction
  • Ectopic expression of the cultivated GIF1 gene with the 35S or rice Waxy promoter resulted in smaller grains, whereas overexpression of GIF1 driven by its native promoter increased grain production
  • Taken together, our study reveals that sugar homeostasis mediated by GIF1 plays an important role in constitutive and induced physical and chemical defence
  • Sugar homeostasis mediated by cell wall invertase GRAIN INCOMPLETE FILLING 1 (GIF1) plays a role in pre-existing and induced defence in rice
  • These findings, together with the domestication signature that we identified by comparing nucleotide diversity of the GIF1 loci between cultivated and wild rice, strongly suggest that GIF1 is a potential domestication gene and that such a domestication-selected gene can be used for further crop improvement
  • In this study, we observed that the grains of gif1, a loss-of-function mutant of the cell wall invertase gene GRAIN INCOMPLETE FILLING 1 (GIF1), were hypersusceptible to postharvest fungal pathogens, with decreased levels of sugars and a thinner glume cell wall in comparison with the wild-type
  • Moreover, the cell wall was much thicker in the infection sites of the GIF1-OE plants when compared with the wild-type plants
  • We previously showed that upregulation of OsGIF1 expression improves rice grain size
  • Overexpression and functional knock-out via a CRISPR/Cas9 strategy revealed that OsGIF1 not only positively regulates the sizes of rice leaf, stem, and grain but also influences rice reproduction
  • Expression profiles based on both qRT-PCR and GUS (-glucuronidase) histochemical staining suggested that OsGIF1 is differentially expressed across various rice tissues, consistent with its roles in regulating the development of multiple rice organs
  • Our results suggest that OsGIF1 plays important roles in vegetative and reproductive developmental processes, with important implications for rice breeding
  • Here, we report pleiotropic effects of OsGIF1 on rice organ size regulation
  • Further histological analysis suggested that OsGIF1 affected rice organ size possibly by regulating cell size
  • Representative of the white-belly phenotype, grains of wb1 showed a higher grain chalkiness rate and degree and a lower 1000-grain weight (decreased by ~34%), in comparison with that of Wild Type (WT)
  • Nipponbare demonstrates that WB1 regulates endosperm development and that different mutations of WB1 disrupt its biological function
  • The wb1 mutant develops a white-belly endosperm and abnormal starch granules in the inner portion of white grains
  • Here, we report the isolation and characterization of a recessive mutation of White Belly 1 (WB1), which regulates rice endosperm development, using a modified MutMap method in the rice mutant wb1
  • Transcript levels analysis of all candidate genes showed that WB1 (Os04t0413500), encoding a cell-wall invertase, was the most probable cause of white-belly endosperm phenotype
• Connection
  • GIF1~OsCIN2~OsGIF1~WB1, OsCIN1, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, Here, we report that GIF1 and another CWI gene OsCIN1 constitute a pair of duplicate genes with differentiated expression and function through independent selection
  • GIF1~OsCIN2~OsGIF1~WB1, OsCIN1, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, RESULTS: Through synteny analysis, we show that GIF1 and another cell-wall invertase gene OsCIN1 were paralogues derived from a segmental duplication originated during genome duplication of grasses
  • GIF1~OsCIN2~OsGIF1~WB1, OsCIN1, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, Results based on analyses of population genetics and gene phylogenetic tree of 25 cultivars and 25 wild rice sequences demonstrated that OsCIN1 was also artificially selected during rice domestication with a fixed mutation in the coding region, in contrast to GIF1 that was selected in the promoter region
  • GIF1~OsCIN2~OsGIF1~WB1, OsCIN1, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, GIF1 and OsCIN1 have evolved into different expression patterns and probable different kinetics parameters of enzymatic activity with the latter displaying less enzymatic activity
  • GIF1~OsCIN2~OsGIF1~WB1, OsCIN1, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, Overexpression of GIF1 and OsCIN1 also resulted in different phenotypes, suggesting that OsCIN1 might regulate other unrecognized biological process
  • GIF1~OsCIN2~OsGIF1~WB1, OsCIN1, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, Our discovery that the duplicated pair of GIF1 and OsCIN1 has experienced sub-functionalization implies that selection could act independently on each duplicate towards different functional specificity, which provides a vivid example for evolution of genetic novelties in a model crop
  • GIF1~OsCIN2~OsGIF1~WB1, OsCIN1, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication, Duplication and independent selection of cell-wall invertase genes GIF1 and OsCIN1 during rice evolution and domestication
  • GIF1~OsCIN2~OsGIF1~WB1, GW2~OsGW2, Seed size is determined by the combinations of the genes controlling different seed characteristics in rice., Rice seed size is an important agronomic trait in determining the yield potential, and four seed size related genes (GS3, GW2, qSW5/GW5 and GIF1) have been cloned in rice so far
  • GIF1~OsCIN2~OsGIF1~WB1, GW2~OsGW2, Seed size is determined by the combinations of the genes controlling different seed characteristics in rice., Additionally, GIF1 expression was found to be positively regulated by qSW5 but negatively by GW2 and GS3
  • GIF1~OsCIN2~OsGIF1~WB1, OsYUC12, Auxin and Cell Wall Invertase Related Signaling during Rice Grain Development., Unlike ZmYUC1, OsYUC12 expression is not reduced in the rice CWIN mutant, gif1
  • GIF1~OsCIN2~OsGIF1~WB1, qGW8~OsSPL16~GW8, Natural Variation in OsMKK3 Contributes to Grain Size and Chalkiness in Rice., We showed that OsSPL16, GS5, and GIF1 have a substantial effect on the OsMKK3-regulated grain size pathway

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