- Information
- Symbol: OsNAC2,OsTIL1,OMTN2,OsORE1
- MSU: LOC_Os04g38720
- RAPdb: Os04g0460600
- Publication
- Overexpression of a NAC-domain protein promotes shoot branching in rice, 2007, New Phytol.
- OsNAC2 encoding a NAC transcription factor affects plant height through mediating the gibberellic acid pathway in rice., 2015, Plant J.
- The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice., 2017, Sci Rep.
- A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis., 2017, Plant Physiol.
- Overexpression of OsmiR164b-resistant OsNAC2 improves plant architecture and grain yield in rice., 2018, J Exp Bot.
- OsNAC2 positively affects salt-induced cell death and binds to the OsAP37 and OsCOX11 promoters., 2018, Plant J.
- OsNAC2 integrates auxin and cytokinin pathways to modulate rice root development., 2019, Plant Biotechnol J.
- Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways., 2019, Rice (N Y).
- OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice, 2020, Rice (N Y).
- OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, 2021, Front Plant Sci.
- Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice., 2022, J Integr Plant Biol.
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Genbank accession number
- Key message
- Overexpression of OsNAC2 regulates shoot branching in rice
- Overexpression of OsNAC2 contributes tiller bud outgrowth, but does not affect tiller bud initiation
- Here, an activation-tagging mutant Ostil1 (Oryza sativa tillering1) was characterized, which showed increased tillers, enlarged tiller angle and semidwarf phenotype
- This suggests that OsNAC2 has potential utility for improving plant structure for higher light-use efficiency and higher yield potential in rice
- OsNAC2 encoding a NAC transcription factor affects plant height through mediating the gibberellic acid pathway in rice.
- Taken together, we proposed that OsNAC2 is a negative regulator of the plant height and flowering time, which acts by directly regulating key genes of the GA pathway in rice
- With reduced yields under drought conditions at the flowering stage, OsNAC2 overexpression lines had lower resistance to high salt and drought conditions
- The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice.
- We further confirmed that OsNAC2 directly binds the promoters of LATE EMBRYOGENESIS ABUNDANT 3 (OsLEA3) and Stress-Activated Protein Kinases 1 (OsSAPK1), two marker genes in the abiotic stress and ABA response pathways, respectively
- Our results suggest that in rice OsNAC2 regulates both abiotic stress responses and ABA-mediated responses, and acts at the junction between the ABA and abiotic stress pathways
- Overexpression of OsNAC2 dramatically accelerated leaf senescence, whereas its knockdown lines showed a delay in leaf senescence
- Additionally, reduced OsNAC2 expression leads to about 10% increase in the grain yield of RNAi lines
- Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly upregulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as downregulating the ABA catabolic gene (OsABA8ox1)
- Interestingly, OsNAC2 is upregulated by a lower level of ABA but downregulated by a higher level of ABA, indicating a feedback repression of OsNAC2 by ABA
- Overexpression of miR164b or down-regulation of OsNAC2 led to decreased panicle length and grain yield in the main panicle
- Overexpression of OsmiR164b-resistant OsNAC2 improves plant architecture and grain yield in rice.
- In summary, we identified an efficient new strategy to substantially increase rice yield and improve plant architecture through over-expression of OsmiR164b-resistant OsNAC2
- Additionally, OsNAC2 expression was enhanced in rice seedlings exposed to a high NaCl concentration
- OsNAC2 positively affects salt-induced cell death and binds to the OsAP37 and OsCOX11 promoters.
- Overall, our results suggest that OsNAC2 accelerates NaCl-induced PCD and provide new insights into the mechanisms affecting ROS accumulation, plant caspase-like activity, and K+efflux
- OsNAC2 integrates auxin and cytokinin pathways to modulate rice root development.
- OsNAC2 predominantly expressed in primary root tips, crown roots and lateral root primordia, implying it influences root development
- Thus, we describe that OsNAC2 functions as an upstream integrator of auxin and cytokinin signals that affect CRL and CDK production to regulate cell division during root development
- Additionally, OsNAC2 can directly bind to the promoters of IAA inactivation-related genes (GH3
- Overexpressing a microRNA (miR164b)-resistant OsNAC2 mutant gene, which generates transcripts that cannot be targeted by miR164b, improves rice plant architecture and yield; however, the performance of these mOsNAC2-overexpressing lines, named ZUOErN3 and ZUOErN4, under abiotic stress conditions such as drought have not yet been fully characterized
- Taken together, our results show that OsNAC2 plays a positive regulatory role in drought and salt tolerance in rice through ABA-mediated pathways
- Moreover, OsNAC2 delays the germination of seeds and coleoptile growth through the ABA pathway instead of the ethylene and GA pathway, by targeting the promoters of OsNCED3, OsZEP1, and OsABA8ox1
- OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling
- Further study proved that OsNAC2 directly activates the expressions of OsACO and OsACO3, enhancing ethylene synthesis, and then retards seedling establishment
- We also found that OsNAC2 regulates downstream targets in a time-dependent manner by binding to the promoter of OsKO2 in the seedling period but not in the germination stage
- Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice.
- Based on these observations, we propose that OsSPL10 confers drought tolerance by regulating OsNAC2 expression and that OsSPL10 (Hap1) could be a valuable haplotype for genetic improvement of drought tolerance in rice
- Connection
- D35~OsKOS3~OsKO2, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 encoding a NAC transcription factor affects plant height through mediating the gibberellic acid pathway in rice., Dual-luciferase reporter analyses, yeast one-hybrid assays and ChIP-qPCR suggested that OsNAC2 directly interacted with the promoter of OsEATB and OsKO2
- OsEATB, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 encoding a NAC transcription factor affects plant height through mediating the gibberellic acid pathway in rice., Dual-luciferase reporter analyses, yeast one-hybrid assays and ChIP-qPCR suggested that OsNAC2 directly interacted with the promoter of OsEATB and OsKO2
- OsLEA3~OsLEA3-1, OsNAC2~OsTIL1~OMTN2~OsORE1, The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice., We further confirmed that OsNAC2 directly binds the promoters of LATE EMBRYOGENESIS ABUNDANT 3 (OsLEA3) and Stress-Activated Protein Kinases 1 (OsSAPK1), two marker genes in the abiotic stress and ABA response pathways, respectively
- OsABA1~OSZEP1, OsNAC2~OsTIL1~OMTN2~OsORE1, A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis., Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly upregulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as downregulating the ABA catabolic gene (OsABA8ox1)
- OsABA8ox1, OsNAC2~OsTIL1~OMTN2~OsORE1, A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis., Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly upregulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as downregulating the ABA catabolic gene (OsABA8ox1)
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsNCED3, A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis., Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly upregulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as downregulating the ABA catabolic gene (OsABA8ox1)
- OsAP37, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 positively affects salt-induced cell death and binds to the OsAP37 and OsCOX11 promoters., OsNAC2 positively affects salt-induced cell death and binds to the OsAP37 and OsCOX11 promoters.
- OsAP37, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 positively affects salt-induced cell death and binds to the OsAP37 and OsCOX11 promoters., Moreover, the results of quantitative real-time PCR, chromatin immunoprecipitation, dual-luciferase, and yeast one-hybrid assays indicated that OsNAC2 targeted genes encoding an ROS scavenger (OsCOX11) and a caspase-like protease (OsAP37)
- OsGORK~OsK5.2, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 positively affects salt-induced cell death and binds to the OsAP37 and OsCOX11 promoters., Furthermore, K+-efflux channels (OsGORK and OsSKOR) were obviously activated by OsNAC2
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsSKOR, OsNAC2 positively affects salt-induced cell death and binds to the OsAP37 and OsCOX11 promoters., Furthermore, K+-efflux channels (OsGORK and OsSKOR) were obviously activated by OsNAC2
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsWRKY5, OsWRKY5 Promotes Rice Leaf Senescence via Senescence-Associated NAC and Abscisic Acid Biosynthesis Pathway., Furthermore, RT-qPCR and yeast one-hybrid analysis demonstrated that OsWRKY5 indirectly upregulates the expression of senescence-associated NAM/ATAF1/2/CUC2 (NAC) genes including OsNAP and OsNAC2
- OsLEA3~OsLEA3-1, OsNAC2~OsTIL1~OMTN2~OsORE1, Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways., Moreover, OsNAC2 directly interacted with the promoters of OsLEA3 and OsNCED3 in yeast one-hybrid assays
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsNCED3, Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways., Moreover, OsNAC2 directly interacted with the promoters of OsLEA3 and OsNCED3 in yeast one-hybrid assays
- D35~OsKOS3~OsKO2, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, We also found that OsNAC2 regulates downstream targets in a time-dependent manner by binding to the promoter of OsKO2 in the seedling period but not in the germination stage
- OsABA1~OSZEP1, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, Moreover, OsNAC2 delays the germination of seeds and coleoptile growth through the ABA pathway instead of the ethylene and GA pathway, by targeting the promoters of OsNCED3, OsZEP1, and OsABA8ox1
- OsABA8ox1, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, Moreover, OsNAC2 delays the germination of seeds and coleoptile growth through the ABA pathway instead of the ethylene and GA pathway, by targeting the promoters of OsNCED3, OsZEP1, and OsABA8ox1
- OsACO3, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, Further study proved that OsNAC2 directly activates the expressions of OsACO and OsACO3, enhancing ethylene synthesis, and then retards seedling establishment
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsNCED3, OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, Moreover, OsNAC2 delays the germination of seeds and coleoptile growth through the ABA pathway instead of the ethylene and GA pathway, by targeting the promoters of OsNCED3, OsZEP1, and OsABA8ox1
- COX11~OsCOX11, OsNAC2~OsTIL1~OMTN2~OsORE1, Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice., Importantly, we demonstrated that the varieties with the OsSPL10 (Hap1) allele show low expression levels of OsSPL10 and its downstream gene, OsNAC2, which decreases the expression of OsAP37 and increases the expression of OsCOX11, thus preventing ROS accumulation and PCD
- OsAP37, OsNAC2~OsTIL1~OMTN2~OsORE1, Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice., Importantly, we demonstrated that the varieties with the OsSPL10 (Hap1) allele show low expression levels of OsSPL10 and its downstream gene, OsNAC2, which decreases the expression of OsAP37 and increases the expression of OsCOX11, thus preventing ROS accumulation and PCD
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsSPL10, Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice., Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice.
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsSPL10, Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice., Importantly, we demonstrated that the varieties with the OsSPL10 (Hap1) allele show low expression levels of OsSPL10 and its downstream gene, OsNAC2, which decreases the expression of OsAP37 and increases the expression of OsCOX11, thus preventing ROS accumulation and PCD
- OsNAC2~OsTIL1~OMTN2~OsORE1, OsSPL10, Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice., Based on these observations, we propose that OsSPL10 confers drought tolerance by regulating OsNAC2 expression and that OsSPL10 (Hap1) could be a valuable haplotype for genetic improvement of drought tolerance in rice
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