Os4BGlu12

2015-01-20 | Categories genes  | Tags cell wall  salinity  shoot  growth  leaf  seedling  stem  sheath  ethylene  salinity stress  defense 

• Information
  • Symbol: Os4BGlu12
  • MSU: LOC_Os04g39880
  • RAPdb: Os04g0474800
• Publication
  • The crystal structure of rice Oryza sativa L. Os4BGlu12, an oligosaccharide and tuberonic acid glucoside-hydrolyzing beta-glucosidase with significant thioglucohydrolase activity, 2011, Arch Biochem Biophys.
  • Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell wall-β-glucan-derived oligosaccharides and glycosides, 2010, Plant Science.
  • Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase, 2006, BMC Plant Biol.
  • Expression, purification, crystallization and preliminary X-ray analysis of rice Oryza sativa L. Os4BGlu12 beta-glucosidase, 2010, Acta Crystallogr Sect F Struct Biol Cryst Commun.
• Genbank accession number
  • AK062776
• Key message
  • Os4BGlu12 expressed in recombinant Escherichia coli hydrolyzed β-(1,3;1,4)-glucooligosaccharides generated by the wounding-induced rice endo-(1,3;1,4)-β-glucanase OsEGL1, suggesting that both enzymes may act in concert in remodeling of damaged cell wall
  • The amino acid residues predicted to line the active site of Os4BGlu12 are more similar to those of cyanogenic and flavonoid β-glucosidases than oligosaccharide hydrolases, and it may function in defense, as well as in cell wall-derived oligosaccharide break-down
  • Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell wall-β-glucan-derived oligosaccharides and glycosides
  • Rice Os4BGlu12 β-glucosidase is a family 1 glycoside hydrolase, the transcript levels of which have previously been found to be induced in response to herbivore attack and salinity stress
  • Here, high levels of Os4bglu12 transcripts were also detected in the shoot during germination, in the leaf sheath and stem of mature rice plants under normal growth conditions
  • The cDNA of the Os4bglu12 gene, which encodes a protein identical at 40 of 44 amino acid residues with the N-terminal sequence of a cell wall-bound enzyme previously purified from germinating rice, was isolated by RT-PCR from rice seedlings
  • To begin this analysis, Os4bglu12 beta-glucosidase was characterized and found to have high exoglucanase activity, consistent with a role in cell wall metabolism
  • Native Os4BGlu12 and its complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside (DNP2FG) were crystallized using 19% polyethylene glycol (3350 or 2000, respectively) in 0
• Connection

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