| Categories genes  | Tags spikelet  map-based cloning  ethylene  floral  floral meristem  nucleus  organ identity  ethylene response  development  R protein  meristem  spikelet meristem  palea  floral organ 
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  • Key message
    • The mof1 mutant has a delayed transition from the spikelet to the floral meristem, inducing the formation of extra lemma-like and palea-like organs
    • Taken together, our results reveal that MOF1 plays an important role in the regulation of organ identity and spikelet determinacy in rice
    • We used map-based cloning to identify the MOF1 locus and confirmed our identification by complementation and by generating new mof1 alleles using CRISPR-Cas9 gene editing
    • MOF1 encodes a MYB domain protein with the typical ethylene response factor-associated amphiphilic repression (EAR) motifs, is expressed in all organs and tissues, and has a strong repression effect
    • MOF1 localizes to the nucleus and interacts with TOPLESS-RELATED PROTEINs (TPRs) to possibly repress the expression of downstream target genes
    • Due to the increase in the number of floral organs and development of extra transformed palea/marginal region of the palea (mrp)-like organs, some mfs2 spikelets had a tendency to produce two florets
    • These defects implied that the mfs2 mutation caused abnormal specification of palea identity and partial loss of spikelet determination
    • The results indicate that MFS2 acts as a repressor that regulates floral organ identities and spikelet meristem determinacy in rice by forming a repression complex with rice TPL/TPR proteins
    • In the mfs2 mutant, specification of palea identity was severely disturbed and showed degradation or transformation into a lemma-like organ, and the number of all floral organs was increased to varying degrees
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