OsSPX-MFS1,OsPSS1

2015-01-20 | Categories genes  | Tags phosphate  shoot  homeostasis  transporter  pi  Pi 

• Information
  • Symbol: OsSPX-MFS1,OsPSS1
  • MSU: LOC_Os04g48390
  • RAPdb: Os04g0573000
• Publication
  • Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, 2014, New Phytol.
  • Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves, 2012, New Phytol.
  • Complex regulation of two target genes encoding SPX-MFS proteins by rice miR827 in response to phosphate starvation, 2010, Plant Cell Physiol.
  • Identification of plant vacuolar transporters mediating phosphate storage., 2016, Nat Commun.
• Genbank accession number
  • AK066068
• Key message
  • Here we report on the characterization of rice osa-miR827 and its two target genes, OsSPX-MFS1 and OsSPX-MFS2, which encode SPX-MFS proteins predicted to be implicated in phosphate (Pi) sensing or transport
  • OsSPX-MFS1 mRNA is mainly expressed in shoots under sufficient Pi supply while its expression is reduced on Pi starvation, revealing a direct relationship between induction of osa-miR827 and down-regulation of OsSPX-MFS1
  • Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves
  • The function of OsSPX-MFS1 in Pi homeostasis was analysed using an OsSPX-MFS1 mutant (mfs1) and osa-miR827 overexpression line (miR827-Oe)
  • Mutation in OsSPX-MFS1 (mfs1) and overexpression of the upstream miR827 (miR827-Oe) plants impaired Pi homeostasis in the leaves
  • • The results suggest that OsSPX-MFS1 is a key player in maintaining Pi homeostasis in the leaves, potentially acting as a Pi transporter
  • • Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced
  • Finally, heterologous complementation of a yeast mutant impaired in Pi transporter was used to assess the capacity of OsSPX-MFS1 to transport Pi
  • In addition, studies in yeast revealed that OsSPX-MFS1 may be involved in Pi transport
  • In parallel, we analyzed the responses of the two OsSPX-MFS1 and OsSPX-MFS2 gene targets to Pi starvation
  • This suggests that OsSPX-MFS1 and OsSPX-MFS2 are both negatively regulated by osa-miR827 abundance although they respond differently to external Pi conditions
  • Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles
• Connection
  • OsARF12, OsSPX-MFS1~OsPSS1, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • LTN1~OsPHO2~OsRLS1, OsSPX-MFS1~OsPSS1, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS2, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS3~OsPSS3, Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves, * Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS2, Complex regulation of two target genes encoding SPX-MFS proteins by rice miR827 in response to phosphate starvation, Here we report on the characterization of rice osa-miR827 and its two target genes, OsSPX-MFS1 and OsSPX-MFS2, which encode SPX-MFS proteins predicted to be implicated in phosphate (Pi) sensing or transport
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS2, Complex regulation of two target genes encoding SPX-MFS proteins by rice miR827 in response to phosphate starvation, In parallel, we analyzed the responses of the two OsSPX-MFS1 and OsSPX-MFS2 gene targets to Pi starvation
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS2, Complex regulation of two target genes encoding SPX-MFS proteins by rice miR827 in response to phosphate starvation, This suggests that OsSPX-MFS1 and OsSPX-MFS2 are both negatively regulated by osa-miR827 abundance although they respond differently to external Pi conditions
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS3~OsPSS3, OsSPX-MFS3, a vacuolar phosphate efflux transporter, is involved in maintaining Pi homeostasis in rice., OsSPX-MFS1, OsSPX-MFS2 and OsSPX-MFS3 all localized on the tonoplast of rice protoplasts, even in the absence of the SPX domain

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