OsARF12

2015-01-20 | Categories genes  | Tags primary root  root  phosphate  auxin  iron  homeostasis  mitochondria  transporter  transcription factor  pi  resistance  flower opening  stigma size  grain  grain size  brassinosteroid  BR  Brassinosteroid  BR signaling  grain filling  grain weight  BR  young panicles 

• Information
  • Symbol: OsARF12
  • MSU: LOC_Os04g57610
  • RAPdb: Os04g0671900
• Publication
  • OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice Oryza sativa, 2012, New Phytol.
  • Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, 2014, New Phytol.
  • Osa-miR167d facilitates infection of Magnaporthe oryzae in rice., 2019, J Integr Plant Biol.
  • Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and IAA Metabolism, 2020, Plant Physiol.
  • Auxin response factors ARFs differentially regulate rice antiviral immune response against rice dwarf virus, 2021, PLoS Pathog.
  • The miR167-OsARF12 module regulates grain filling and grain size downstream of miR159., 2023, Plant Commun.
• Genbank accession number
  • AK071455
• Key message
  • Here, we found a limiting factor of RSA–OsARF12–an auxin response factor whose knockout led to decreased primary root length in rice (Oryza sativa)
  • • The root elongation zones of osarf12 and osarf12/25, which had lower auxin concentrations, were distinctly shorter than for the wild-type, possibly as a result of decreased expression of auxin synthesis genes OsYUCCAs and auxin efflux carriers OsPINs and OsPGPs
  • The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content
  • • The data provide evidence for the biological function of OsARF12, which is implicated in regulating root elongation
  • OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)
  • Transcript analysis revealed that Pi-responsive genes–Phosphate starvation (OsIPS)1 and OsIPS2, SYG1/Pho81/XPR1(OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3 MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1)–were more abundant in the osarf12 and osarf12/25 mutants under +Pi/-Pi conditions
  • Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa)
  • Here, we report that an auxin response factor, OsARF12, functions in Pi homeostasis
  • Results from -Pi/1-naphthylphthalamic acid (NPA) treatments, and auxin reporter DR5::GUS staining suggest that root system alteration and Pi-induced auxin response were at least partially controlled by OsARF12
  • • OsARF12 as a transcription activator can facilitate the expression of the auxin response element DR5::GFP, and OsARF12 was inhibited by osa-miRNA167d by transient expression in tobacco and rice callus
  • These findings enrich our understanding of the biological functions of OsARF12, which also acts in regulating Pi homeostasis
  • Measurement of element content, quantitative reverse transcription polymerase chain reaction analysis and acid phosphatases (APases) activity assay showed that the osarf12 mutant and osarf12/25 double mutant with P-intoxicated phenotypes had higher P concentrations, up-regulation of the Pi transporter encoding genes and increased APase activity under Pi-sufficient/-deficient (+Pi/-Pi, 0
  • Strikingly, our genetic data showed that loss-of-function mutants of osarf12 or osarf16 exhibit reduced resistance whereas osarf11 mutants display enhanced resistance to RDV
  • In turn, OsARF12 activates the down-stream OsWRKY13 expression through direct binding to its promoter, loss-of-function mutants of oswrky13 exhibit reduced resistance
  • miR167d-ARFs Module Regulates Flower Opening and Stigma Size in Rice
  • Further analysis indicates that OsARF12 is the major mediator of miR167 in regulating rice grain filling
  • Expectedly, over expressing OsARF12 could resemble the phenotype of STTM/MIM167 plants with respect to grain weight and grain filling rate
  • Flow cytometric analysis in young panicles of plants overexpressing OsARF12 and cell number examination of cdkf;2 mutants verify that OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;2
  • Additionally, plants overexpressing OsARF12 or cdkf;2 mutants present enhanced or reduced sensitivity to exogenous auxin and brassinosteroid (BR) treatments, confirming that OsCDKF;2 targeting by OsARF12 mediates auxin and BR signaling
• Connection
  • OsARF12, Osspr1, OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice Oryza sativa, The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content
  • OsARF12, OsIRT1, OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice Oryza sativa, The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content
  • LTN1~OsPHO2~OsRLS1, OsARF12, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • OsARF12, OsSPX-MFS1~OsPSS1, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • OsARF12, OsSPX-MFS2, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • OsARF12, OsSPX1, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Transcript analysis revealed that Pi-responsive genes–Phosphate starvation (OsIPS)1 and OsIPS2, SYG1/Pho81/XPR1(OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3 MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1)–were more abundant in the osarf12 and osarf12/25 mutants under +Pi/-Pi conditions
  • OsARF12, OsTIR1, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Transcript analysis revealed that Pi-responsive genes–Phosphate starvation (OsIPS)1 and OsIPS2, SYG1/Pho81/XPR1(OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3 MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1)–were more abundant in the osarf12 and osarf12/25 mutants under +Pi/-Pi conditions
  • OsARF12, OsmiR167a, OsmiR167a-targeted auxin response factors modulate tiller angle via fine-tuning auxin distribution in rice., In the overexpression of OsMIR167a plants, the expression of OsARF12, OsARF17 and OsARF25 were severely repressed, and displayed larger tiller angle as well as the osarf12/osarf17 and osarf12/ osarf25 plants
  • OsARF12, OsmiR167a, OsmiR167a-targeted auxin response factors modulate tiller angle via fine-tuning auxin distribution in rice., Our results reveal that OsmiR167a represses its targets, OsARF12, OsARF17 and OsARF25, to control rice tiller angle by fine-tuning auxin asymmetric distribution in shoots
  • OsARF12, OsGRF7, Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and IAA Metabolism , Further analysis indicated that OsGRF7 binds the ACRGDA motif in the promoters of Cytochrome P450 gene (OsCYP714B1) and AUXIN RESPONSE FACTOR 12 (OsARF12), which are involved in the gibberellin synthesis and auxin signaling pathways, respectively
  • OsARF12, OsGRF7, Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and IAA Metabolism, Further analysis indicated that OsGRF7 binds the ACRGDA motif in the promoters of Cytochrome P450 gene (OsCYP714B1) and AUXIN RESPONSE FACTOR 12 (OsARF12), which are involved in the gibberellin synthesis and auxin signaling pathways, respectively
  • OsARF11, OsARF12, Auxin response factors ARFs differentially regulate rice antiviral immune response against rice dwarf virus, Strikingly, our genetic data showed that loss-of-function mutants of osarf12 or osarf16 exhibit reduced resistance whereas osarf11 mutants display enhanced resistance to RDV
  • OsARF12, OsARF16, Auxin response factors ARFs differentially regulate rice antiviral immune response against rice dwarf virus, Strikingly, our genetic data showed that loss-of-function mutants of osarf12 or osarf16 exhibit reduced resistance whereas osarf11 mutants display enhanced resistance to RDV
  • OsARF12, OsWRKY13~WRKY13, Auxin response factors ARFs differentially regulate rice antiviral immune response against rice dwarf virus, In turn, OsARF12 activates the down-stream OsWRKY13 expression through direct binding to its promoter, loss-of-function mutants of oswrky13 exhibit reduced resistance
  • OsARF12, OsCDKF;2, The miR167-OsARF12 module regulates grain filling and grain size downstream of miR159., Upon in-depth analysis, we found that OsARF12 activates OsCDKF;2 expressions by directly binding to the TGTCGG motif in the promoter region
  • OsARF12, OsCDKF;2, The miR167-OsARF12 module regulates grain filling and grain size downstream of miR159., Flow cytometric analysis in young panicles of plants overexpressing OsARF12 and cell number examination of cdkf;2 mutants verify that OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;2
  • OsARF12, OsCDKF;2, The miR167-OsARF12 module regulates grain filling and grain size downstream of miR159., Additionally, plants overexpressing OsARF12 or cdkf;2 mutants present enhanced or reduced sensitivity to exogenous auxin and brassinosteroid (BR) treatments, confirming that OsCDKF;2 targeting by OsARF12 mediates auxin and BR signaling

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