OsCASP1

• Symbol: OsCASP1
• MSU: LOC_Os04g58760
• RAPdb: Os04g0684300

• OsCASP1 is required for Casparian strip formation at endodermal cells of rice roots for selective uptake of mineral elements., 2019, Plant Cell.
• Overexpression of OsCASP1 Improves Calcium Tolerance in Rice, 2021, Int J Mol Sci.
• Radial transport difference mediated by root endodermal barriers contributes to differential cadmium accumulation between japonica and indica subspecies of rice Oryza sativa L.., 2021, J Hazard Mater.
• Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis., 2022, Front Plant Sci.

Genbank accession number

• OsCASP1 was mainly expressed in the root elongation zone, and the protein encoded was first localized to all side of the plasma membrane of endodermal cells without CS, followed by to the middle of anticlinal side of endodermal cells with CS
• These results indicated that OsCASP1 is required for CS formation at the endodermis and that the CS in rice plays an important role in root selective uptake of mineral elements especially Ca and Si
• Knockout of OsCASP1 resulted in defect of CS formation at the endodermis and decreased growth under both soil and hydroponic conditions
• Overexpression of OsCASP1 Improves Calcium Tolerance in Rice
• Here, we demonstrated that OsCASP1 overexpression enhanced Ca tolerance in rice
• Our findings suggest that OsCASP1 could play an important role in nutrient homeostasis and adaptation to the growth environment
• Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis.
• Here, we observed the structure of rice primary and small lateral roots (SLRs), particularly the deposition patterns of lignin and suberin in wild type and Oscasp1 mutants
• The loss of OsCASP1 function alters the expression of the genes involved in suberin biosynthesis and the deposition of suberin in the endodermis and sclerenchyma and leads to delayed CS formation and uneven lignin deposition in SLRs

• OsCASP1, ZN, OsCASP1 is required for Casparian strip formation at endodermal cells of rice roots for selective uptake of mineral elements., Mineral analysis showed that the oscasp1 mutants accumulated higher Ca, but less Mn, Zn, Fe, Cd and As in the shoots compared with the wild type
• OsCASP1, OsCASP2, OsCASP1 forms complexes with itself and OsCASP2 in rice., OsCASP1 forms complexes with itself and OsCASP2 in rice.
• OsCASP1, OsCASP2, OsCASP1 forms complexes with itself and OsCASP2 in rice., Expression analysis shows that OsCASP1, OsCASP2, OsCASP3, and OsCASP5 were expressed in roots apart from OsCASP4
• OsCASP1, OsCASP2, OsCASP1 forms complexes with itself and OsCASP2 in rice., A yeast two-hybrid (Y2H) assay revealed that OsCASP1 can interact with itself and OsCASP2, but not with OsCASP3 and OsCASP5
• OsCASP1, OsCASP2, OsCASP1 forms complexes with itself and OsCASP2 in rice., These interactions of OsCASP1 with itself and OsCASP2 at the plasma membrane were confirmed using bimolecular fluorescence complementation (BiFC) in rice protoplasts
• OsCASP1, OsCASP2, OsCASP1 forms complexes with itself and OsCASP2 in rice., These results indicated that OsCASP1 can form complexes with itself and OsCASP2 in rice roots
• OsCASP1, OsPAL, Radial transport difference mediated by root endodermal barriers contributes to differential cadmium accumulation between japonica and indica subspecies of rice Oryza sativa L.., The lignin/suberin content in japonica subspecies was 47%/42% greater than that in indica subspecies because of the higher expression of lignin/suberin biosynthesis-related genes (OsCASP1, OsPAL, OsCYP86A1 and OsKCS20)
• OsCASP1, OsMYB36c, Three OsMYB36 members redundantly regulate Casparian strip formation at the root endodermis, Three OsMYB36s regulate OsCASP1 and OsESB1 expression by directly binding to MYB-binding motifs in their promoters.
• OsCASP1, OsMYB36a, Three OsMYB36 members redundantly regulate Casparian strip formation at the root endodermis, Three OsMYB36s regulate OsCASP1 and OsESB1 expression by directly binding to MYB-binding motifs in their promoters.
• OsCASP1, OsMYB36b, Three OsMYB36 members redundantly regulate Casparian strip formation at the root endodermis, Three OsMYB36s regulate OsCASP1 and OsESB1 expression by directly binding to MYB-binding motifs in their promoters.