OsRLCK176,BML

• Symbol: OsRLCK176,BML
• MSU: LOC_Os05g02020
• RAPdb: Os05g0110900

• OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity, 2014, Plant J.
• Four receptor-like cytoplasmic kinases regulate development and immunity in rice., 2015, Plant Cell Environ.
• The Brown Midrib Leaf bml Mutation in Rice Oryza sativa L. Causes Premature Leaf Senescence and the Induction of Defense Responses., 2018, Genes (Basel).

Genbank accession number

• Here, we demonstrate that the rice LysM receptor-like kinase OsCERK1, a key component of the chitin elicitor signaling pathway, also plays an important role in PGN-triggered immunity in rice.
• BML is a candidate gene associated with leaf senescence, ROS regulation, and disease response, also involved in hormone signaling in rice
• The bml mutant showed excessive accumulation of reactive oxygen species (ROS), increased activities of superoxide dismutase, catalase, and malondialdehyde, upregulation of senescence-induced STAY-GREEN genes and senescence-related transcription factors, and down regulation of photosynthesis-related genes
• The bml mutants had brown midribs in their leaves and initiated senescence prematurely, at the onset of heading
• In pathogen response, bml demonstrated higher resistance against Xanthomonas oryzae pv
• A map-based cloning using insertion/deletion markers confirmed that BML was located in the 57
• The levels of abscisic acid (ABA) and jasmonic acid (JA) were increased in bml with the upregulation of some ABA and JA biosynthetic genes

• OsCERK1, OsRLCK176~BML, OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity, Furthermore, the receptor-like cytoplasmic kinase OsRLCK176 functions downstream of OsCERK1 in the PGN and chitin signaling pathways, suggesting that these MAMPs share overlapping intracellular signaling components.
• OsRLCK107, OsRLCK176~BML, Four receptor-like cytoplasmic kinases regulate development and immunity in rice., Here, we report the functional characterization of four rice RLCKs-OsRLCK57, OsRLCK107, OsRLCK118, and OsRLCK176 from subfamily VII
• OsRLCK118, OsRLCK176~BML, Four receptor-like cytoplasmic kinases regulate development and immunity in rice., Here, we report the functional characterization of four rice RLCKs-OsRLCK57, OsRLCK107, OsRLCK118, and OsRLCK176 from subfamily VII
• OsRLCK176~BML, OsRLCK57, OsRLCK 57, OsRLCK107 and OsRLCK118 Positively Regulate Chitin- and PGN-Induced Immunity in Rice., OsRLCK57, OsRLCK107 and OsRLCK118, positively regulate chitin- and PGN- induced responses in rice, similar to that observed in OsRLCK176
• OsCPK4, OsRLCK176~BML, The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity., OsCPK4 was further identified to interact with a receptor-like cytoplasmic kinase OsRLCK176
• OsCPK4, OsRLCK176~BML, The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity., OsRLCK176 accumulation is negatively regulated by OsCPK4
• OsCPK4, OsRLCK176~BML, The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity., Interestingly, the kinase-dead OsCPK4 promotes OsRLCK176 degradation more strongly than the wild-type protein
• OsCPK4, OsRLCK176~BML, The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity., OsCPK4 and OsRLCK176 mutually phosphorylate each other and form a feedback loop
• OsCPK4, OsRLCK176~BML, The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity., Moreover, the kinase activity and phosphorylation of OsCPK4 and OsRLCK176 contribute to the stability of OsRLCK176
• OsCPK4, OsRLCK176~BML, The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity., These findings indicate that the kinase-inactive OsCPK4 promotes OsRLCK176 degradation and restricts plant defenses; whereas the activation of OsCPK4-OsRLCK176 phosphorylation circuit invalidates the OsRLCK176 degradation machinery and thus enhancing plant immunity