- Information
- Symbol: RSR1,qHD5
- MSU: LOC_Os05g03040
- RAPdb: Os05g0121600
- Publication
- Genbank accession number
- Key message
- Seeds of the knockout mutant rsr1 consistently show the increased amylose content and altered fine structure of amylopectin and consequently form the round and loosely packed starch granules, resulting in decreased gelatinization temperature
- In contrast, RSR1 overexpression suppresses the expression of starch synthesis genes, resulting in altered amylopectin structure and increased gelatinization temperature
- Interestingly, a decreased proportion of A chains in rsr1 results in abnormal starch granules but reduced gelatinization temperature, whereas an increased proportion of A chains in RSR1-overexpressing plants leads to higher gelatinization temperatures, which is novel and different from previous reports, further indicating the complicated regulation of starch synthesis and determination of the physicochemical properties of starch
- These results demonstrate the potential of coexpression analysis for studying rice starch biosynthesis and the regulation of a complex metabolic pathway and provide informative clues, including the characterization of RSR1, to facilitate the improvement of rice quality and nutrition
- In addition, rsr1 mutants have a larger seed size and increased seed mass and yield
- Among these genes, Rice Starch Regulator1 (RSR1), an APETALA2/ethylene-responsive element binding protein family transcription factor, was found to negatively regulate the expression of type I starch synthesis genes, and RSR1 deficiency results in the enhanced expression of starch synthesis genes in seeds
- We fine-mapped qHD5 and identified LOC_Os05g03040, a gene that encodes an AP2 transcription factor, as the candidate gene of qHD5 in our previous study
- qHD5 may have been selected during indica rice domestication
- Transient expression showed that the qHD5 protein localizes to the nucleus
- All of these results show that qHD5 represses the heading date in rice
- qRT-PCR analysis revealed that qHD5 represses flowering by down-regulating Ehd2
- Connection
- OsSRT1, RSR1~qHD5, OsSRT1 is involved in rice seed development through regulation of starch metabolism gene expression, Down-regulation of OsSRT1 induced higher expression of Rice Starch Regulator1 (RSR1) and amylases genes in developing seeds, which resulted in a decrease of starch synthesis and an increase of starch degradation, leading to abnormal seed development
- Ehd2~RID1, RSR1~qHD5, qHD5 encodes an AP2 factor that suppresses rice heading by down-regulating Ehd2 expression., qHD5 encodes an AP2 factor that suppresses rice heading by down-regulating Ehd2 expression.
- Ehd2~RID1, RSR1~qHD5, qHD5 encodes an AP2 factor that suppresses rice heading by down-regulating Ehd2 expression., qRT-PCR analysis revealed that qHD5 represses flowering by down-regulating Ehd2
- OsLMP1~OsUBP2~LMM22, RSR1~qHD5, Ubiquitin-Specific Protease 2 OsUBP2 Negatively Regulates Cell Death and Disease Resistance in Rice., Map-based cloning showed that the mutated gene in rsr1 encodes a Ubiquitin-Specific Protease 2 (OsUBP2)
- OsLMP1~OsUBP2~LMM22, RSR1~qHD5, Ubiquitin-Specific Protease 2 OsUBP2 Negatively Regulates Cell Death and Disease Resistance in Rice., The ubiquitinated proteomics data of rsr1 and WT provide some possible target protein candidates for OsUBP2
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