EUI1,OsCYP714D1,LG5

2015-01-20 | Categories genes  | Tags growth  gibberellin  cell elongation  breeding  panicle  homeostasis  ga  dwarf  height  plant height  GA  shoot  resistance  salt  tolerance  salt tolerance  salt stress  stress  plant growth  ga  biomass  Gibberellin  biomass production  GA biosynthesis  leaf  grain  grain size  cell proliferation  leaf angle  cell expansion 

• Information
  • Symbol: EUI1,OsCYP714D1,LG5
  • MSU: LOC_Os05g40384
  • RAPdb: Os05g0482400
• Publication
  • Identification of a 98-kb DNA segment containing the rice Eui gene controlling uppermost internode elongation, and construction of a TAC transgene sublibrary, 2004, Mol Genet Genomics.
  • Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice, 2008, Cell Res.
  • Altered disease development in the eui mutants and Eui overexpressors indicates that gibberellins negatively regulate rice basal disease resistance, 2008, Mol Plant.
  • The APETALA-2-Like Transcription Factor OsAP2-39 Controls Key Interactions between Abscisic Acid and Gibberellin in Rice, 2010, PLoS Genet.
  • ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice, 2006, Plant Cell.
  • EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice, 2006, Plant Cell Physiol.
  • Fine mapping and in silico isolation of the EUI1 gene controlling upper internode elongation in rice, 2006, Plant Mol Biol.
  • The Intronic cis-Element SE1 Recruits trans-acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice., 2018, Mol Plant.
  • OsCYP714D1 improves plant growth and salt tolerance through regulating gibberellin and ion homeostasis in transgenic poplar., 2021, Plant Physiol Biochem.
  • LG5, a Novel Allele of EUI1, Regulates Grain Size and Flag Leaf Angle in Rice., 2023, Plants (Basel).
• Genbank accession number
  • AK109526
• Key message
  • It was found that the level of active gibberellin was elevated in the mutant, whereas its growth in response to gibberellin is similar to that of the wild type, suggesting that the higher level accumulation of gibberellin in the eui1 mutant causes the abnormal elongation of the uppermost internode
  • Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes, which could be partially reversed by supplementation with gibberellin
  • Furthermore, apart from the alteration of expression levels of the gibberellin biosynthesis genes, accumulation of SLR1 protein was found in the overexpressing transgenic plants, indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis
  • Therefore, we proposed that EUI1 plays a negative role in gibberellin-mediated regulation of cell elongation in the uppermost internode of rice
  • EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice
  • Here, we report a detailed characterization of an elongated uppermost internode1 (eui1) mutant, which has been used in hybrid rice breeding
  • We cloned the EUI1 gene, which encodes a putative cytochrome P450 monooxygenase, by map-based cloning and found that EUI1 was weakly expressed in most tissues, but preferentially in young panicles
  • The expression of Eui1 is finely tuned, thereby maintaining homeostasis of endogenous bioactive GA and producing plants of normal plant height
  • This complex generates closed chromatin at Eui1, suppressing Eui1 expression and modulating GA homeostasis
  • Here, we identified a dominant dwarf mutant, dEui1, caused by the deletion of an RY motif-containing cis silencing element (SE1) in the intron of Eui1
  • OsCYP714D1 improves plant growth and salt tolerance through regulating gibberellin and ion homeostasis in transgenic poplar.
  • Previously, we reported that expression of the rice cytochrome P450 monooxygenase gene OsCYP714D1 increased gibberellic acid (GA) accumulation and shoot growth in transgenic poplar
  • Our findings imply that OsCYP714D1 participated in the regulation of both shoot growth and salt resistance through regulating gibberellin and ion homeostasis in transgenic poplar, and it can be used as a candidate gene for the engineering of new tree varieties with improved biomass production and salt stress resistance
  • In this work, we demonstrate that expression of OsCYP714D1 improved the salt tolerance of transgenic poplar plants
  • Transcriptomic analyses revealed that OsCYP714D1 expression up-regulated the expressions of GA biosynthesis, signaling and stress responsive genes in transgenic plants under both normal and high salt stress conditions
  • Functional complementation and overexpression tests showed that LG5 can rescue the bigger grain size and larger flag leaf angle in the Xiushui11 (XS) background
  • Knockdown of the LG5 transcription level by RNA interference resulted in elevated grain size and flag leaf angle in the Nipponbare (NIP) background
  • Morphological and cellular analyses suggested that LG5 regulated grain size and flag leaf angle by promoting cell expansion and cell proliferation
• Connection
  • EUI1~OsCYP714D1~LG5, SLR1~OsGAI~OsSLR1, EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice, Furthermore, apart from the alteration of expression levels of the gibberellin biosynthesis genes, accumulation of SLR1 protein was found in the overexpressing transgenic plants, indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis
  • EUI1~OsCYP714D1~LG5, HOX12~Oshox12, Rice HOX12 Regulates Panicle Exsertion by Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1., In this study, we identified a dominant panicle enclosure mutant regulator of eui1 (ree1-D), which is caused by the activation of a homeodomain-leucine zipper transcription factor HOX12
  • EUI1~OsCYP714D1~LG5, HOX12~Oshox12, Rice HOX12 Regulates Panicle Exsertion by Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1., Diminished HOX12 expression by RNA interference enhances the panicle exsertion, mimicking the eui1 phenotype
  • EUI1~OsCYP714D1~LG5, HOX12~Oshox12, Rice HOX12 Regulates Panicle Exsertion by Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1., The expression of EUI1 is elevated in the ree1-D mutant but lowered in HOX12 knockdown plants
  • EUI1~OsCYP714D1~LG5, HOX12~Oshox12, Rice HOX12 Regulates Panicle Exsertion by Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1., Interestingly, both HOX12 and EUI1 genes are predominantly expressed in panicles, where GA4 is highly accumulated
  • EUI1~OsCYP714D1~LG5, HOX12~Oshox12, Rice HOX12 Regulates Panicle Exsertion by Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1., Yeast one-hybrid, electrophoretic mobility shift assay, and chromatin immunoprecipitation analyses showed that HOX12 physically interacts with EUI1 promoter both in vitro and in vivo
  • EUI1~OsCYP714D1~LG5, HOX12~Oshox12, Rice HOX12 Regulates Panicle Exsertion by Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1., Furthermore, overexpression of HOX12 in eui1 mutant background remains the elongated uppermost internode phenotype
  • EUI1~OsCYP714D1~LG5, HOX12~Oshox12, Rice HOX12 Regulates Panicle Exsertion by Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1., These results reveal a novel regulatory module that HOX12 acts directly through EUI1 to regulate panicle exsertion in rice
  • EUI1~OsCYP714D1~LG5, OsVAL2, The Intronic cis-Element SE1 Recruits trans-acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice., Detailed genetic and molecular analysis of SE1 revealed that this intronic cis-element recruits at least a trans-acting repressor complex, containing the B3 repressors OsVAL2 and OsGD1, the SAP18 co-repressor, and a histone deacetylase OsHDA710, to negatively regulate the expression of Eui1
  • EUI1~OsCYP714D1~LG5, OsMADS57, Rice transcription factor OsMADS57 regulates plant height by modulating gibberellin catabolism., The level of EUI1 transcripts involved in GA deactivation was also increased in OsMADS57 knockdown plants
  • EUI1~OsCYP714D1~LG5, OsMADS57, Rice transcription factor OsMADS57 regulates plant height by modulating gibberellin catabolism., More importantly, dual-luciferase reporter assay and electrophoretic mobility shift assay showed that OsMADS57 directly regulates the transcription of OsGA2ox3 as well as EUI1 through binding to the CArG-box motifs in their promoter regions

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