- Information
- Symbol: HSP101,OsClpB-cyt,HSP100
- MSU: LOC_Os05g44340
- RAPdb: Os05g0519700
- Publication
- Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types, 2003, Plant Mol Biol.
- OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB Hsp100 gene in rice Oryza sativa L., 2012, Cell Stress Chaperones.
- Genome-wide analysis of rice ClpB/HSP100, ClpC and ClpD genes, 2010, BMC Genomics.
- A positive feedback loop between HEAT SHOCK PROTEIN101 and HEAT STRESS-ASSOCIATED 32-KD PROTEIN modulates long-term acquired thermotolerance illustrating diverse heat stress responses in rice varieties, 2014, Plant Physiol.
- Complexity of rice Hsp100 gene family: lessons from rice genome sequence data, 2007, J Biosci.
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Genbank accession number
- Key message
- Our results showed that a positive feedback loop formed by two heat-inducible genes, HEAT SHOCK PROTEIN101 (HSP101) and HEAT STRESS-ASSOCIATED 32-KD PROTEIN (HSA32), at the posttranscriptional level prolongs the effect of heat acclimation in rice seedlings
- Rice hsp101 cDNA expression in hsp104 deficient yeast also caused recovery in tolerance against arsenite
- The interplay between HSP101 and HSA32 also affects basal thermotolerance of rice seeds
- In the temperature regime tested, 45 degrees C treatment to intact rice seedlings for 2 h showed maximal levels of hsp101 mRNA
- Transgenic rice plants produced with 2 kb OsClpB-cyt promoter driving Gus reporter gene showed heat- and metal-regulated Gus expression in vegetative tissues and constitutive Gus expression in calli, flowering tissues, and embryonal half of seeds
- OsClpB-cyt transcript is shown to be enriched at milk and dough stages of seed development
- Rice seedlings regenerated with OsClpB-cyt promoter fragment with deletion of its canonical heat shock element sequence (HSE(-273 to -280)) showed not only heat shock inducibility of Gus transcript/protein but also constitutive expression of Gus in vegetative tissues
- While Os05g44340 encodes cytoplasmic Hsp100 protein, those encoded by the other four genes are predicted to have chloroplast transit peptides
- Connection
- HSP101~OsClpB-cyt~HSP100, OsHSF6~OsHsfA2c~OsHsfA6a, OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB Hsp100 gene in rice Oryza sativa L., Yeast one-hybrid assays suggested that OsHsfA2c specifically interacts with OsClpB-cyt promoter
- HSP101~OsClpB-cyt~HSP100, OsHSF6~OsHsfA2c~OsHsfA6a, OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB Hsp100 gene in rice Oryza sativa L., OsHsfA2c also showed binding with OsClpB-cyt and OsHsfB4b showed binding with OsClpB-cyt; notably, interaction of OsHsfB4b was seen for all three OsClpB/Hsp100 protein isoforms (i
- HSP101~OsClpB-cyt~HSP100, OsHSF6~OsHsfA2c~OsHsfA6a, OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB Hsp100 gene in rice Oryza sativa L., OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)
- HSP101~OsClpB-cyt~HSP100, OsHsfB4b, OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB Hsp100 gene in rice Oryza sativa L., OsHsfA2c also showed binding with OsClpB-cyt and OsHsfB4b showed binding with OsClpB-cyt; notably, interaction of OsHsfB4b was seen for all three OsClpB/Hsp100 protein isoforms (i
- HSP101~OsClpB-cyt~HSP100, OsHsfB4b, OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB Hsp100 gene in rice Oryza sativa L., OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)
- HSA32, HSP101~OsClpB-cyt~HSP100, A positive feedback loop between HEAT SHOCK PROTEIN101 and HEAT STRESS-ASSOCIATED 32-KD PROTEIN modulates long-term acquired thermotolerance illustrating diverse heat stress responses in rice varieties, Our results showed that a positive feedback loop formed by two heat-inducible genes, HEAT SHOCK PROTEIN101 (HSP101) and HEAT STRESS-ASSOCIATED 32-KD PROTEIN (HSA32), at the posttranscriptional level prolongs the effect of heat acclimation in rice seedlings
- HSA32, HSP101~OsClpB-cyt~HSP100, A positive feedback loop between HEAT SHOCK PROTEIN101 and HEAT STRESS-ASSOCIATED 32-KD PROTEIN modulates long-term acquired thermotolerance illustrating diverse heat stress responses in rice varieties, The interplay between HSP101 and HSA32 also affects basal thermotolerance of rice seeds
- HSA32, HSP101~OsClpB-cyt~HSP100, A positive feedback loop between HEAT SHOCK PROTEIN101 and HEAT STRESS-ASSOCIATED 32-KD PROTEIN modulates long-term acquired thermotolerance illustrating diverse heat stress responses in rice varieties, The HSP101 and HSA32 protein levels were substantially higher in cv Nipponbare than in cv N22 after a long recovery following heat acclimation treatment, at least partly explaining the difference in the LAT phenotype
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