LTN1,OsPHO2,OsRLS1

2015-01-20 | Categories genes  | Tags root  growth  reproductive  transporter  seedling  shoot  architecture  adventitious root  pi  leaf  phosphorus  phosphate  root architecture  nitrogen  development  homeostasis  flowering time  Pi  Pi homeostasis  Pi signaling  xylem  seed  pi 

• Information
  • Symbol: LTN1,OsPHO2,OsRLS1
  • MSU: LOC_Os05g48390
  • RAPdb: Os05g0557700
• Publication
  • Fine characterization of OsPHO2 knockout mutants reveals its key role in Pi utilization in rice, 2014, J Plant Physiol.
  • Molecular mechanisms regulating Pi-signaling and Pi homeostasis under OsPHR2, a central Pi-signaling regulator, in rice, 2011, Frontiers in Biology.
  • OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, 2010, Plant J.
  • Involvement of OsSPX1 in phosphate homeostasis in rice, 2009, Plant J.
  • Regulation of OsSPX1 and OsSPX3 on expression of OsSPX domain genes and Pi-starvation signaling in rice, 2009, J Integr Plant Biol.
  • Down-regulation of OsSPX1 causes high sensitivity to cold and oxidative stresses in rice seedlings, 2013, PLoS One.
  • Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, 2014, New Phytol.
  • LEAF TIP NECROSIS1 plays a pivotal role in the regulation of multiple phosphate starvation responses in rice, 2011, Plant Physiol.
  • A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-replete rice, 2012, Plant Physiol.
  • Molecular interaction between PHO2 and GI in rice., 2017, Plant Cell Environ.
  • Knocking Out the Gene RLS1 Induces Hypersensitivity to Oxidative Stress and Premature Leaf Senescence in Rice., 2018, Int J Mol Sci.
  • Phosphate-Starvation-Inducible S-Like RNase Genes in Rice Are Involved in Phosphate Source Recycling by RNA Decay, 2020, Front Plant Sci.
  • PROTEIN PHOSPHATASE95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice[OPEN], 2021, Plant Cell.
  • Distribution of phenanthrene in the ospho2 reveals the involvement of phosphate on phenanthrene translocation and accumulation in rice., 2022, Ecotoxicol Environ Saf.
• Genbank accession number
  • AK241747
• Key message
  • The cells in the elongation zone of ospho2 seedling roots were much shorter than those of the wild type
  • The ospho2 mutants exhibited defects in growth and reproductive development in the whole growing period
  • Here we report that OsPHR2 positively regulates the low-affinity Pi transporter gene OsPT2 by physical interaction and upstream regulation of OsPHO2 in roots
  • Our data also show that OsSPX1 is a negative regulator of OsPHR2 and is involved in the feedback of Pi-signaling network in roots that is defined by OsPHR2 and OsPHO2
  • In association with enhanced Pi uptake and transport, some Pi transporters were up-regulated in the ltn1 mutant in the presence of sufficient Pi
  • This study thus provides evidence that OsPHO2, which functions at the downstream of OsPHF1, modulates Pi utilization by regulating the expression of Pht1 transporters in rice
  • Furthermore, the elongation of primary and adventitious roots was enhanced in the ltn1 mutant under Pi starvation, suggesting that LTN1 is involved in Pi-dependent root architecture alteration
  • These results suggest that ospho2 mutant phenotype results from a partial defect in Pi translocation and remobilization in the shoot of rice
  • OsPHR2 positively regulates the low-affinity Pi transporter OsPT2 through physical interaction and reciprocal regulation of OsPHO2 in roots
  • The ltn1 mutant exhibited increased Pi uptake and translocation, which led to Pi overaccumulation in shoots
  • Furthermore, OsPT1 expression was strongly enhanced by the mutation of Phosphate Overaccumulator2 (OsPHO2) but not by Phosphate Starvation Response2, indicating that OsPT1 is involved in the OsPHO2-regulated Pi pathway
  • Furthermore, Pi levels in the ospho2 mutants were highest in the oldest leaf and lowest in the youngest leaf, whereas there was no significant difference in the corresponding leaves of wild-type plants
  • OsSPX1 suppresses the regulation on expression of OsPT2 by OsPHR2 and the accumulation of excess shoot Pi, but it does not suppress induction of OsPT2 or the accumulation of excessive shoot Pi in the Ospho2 mutant
  • The phosphorus concentration in the blades of ospho2 mutants was 5
  • Map-based cloning identified LTN1 as LOC_Os05g48390, the putative ortholog of Arabidopsis PHO2, which plays important roles in Pi starvation signaling
  • Under Pi-sufficient conditions, typical Pi starvation responses such as stimulation of phosphatase and RNase activities, lipid composition alteration, nitrogen assimilation repression, and increased metal uptake were also activated in ltn1
  • Our results strongly indicate that LTN1 is a crucial Pi starvation signaling component downstream of miR399 involved in the regulation of multiple Pi starvation responses in rice
  • In this work, a rice leaf tip necrosis1 (ltn1) mutant was identified and characterized
  • Fine characterization of OsPHO2 knockout mutants reveals its key role in Pi utilization in rice
  • Previous research using forward genetics approaches demonstrated that OsPHO2 regulates multiple phosphate-starvation responses in rice
  • In this work, we finely characterized two independent OsPHO2 knockout rice mutants under inorganic phosphate (Pi)-sufficient conditions
  • Reduced growth, leaf tip necrosis, delayed flowering and over-accumulation of Pi in leaves compared to wild-type were shared features of Osgi and Ospho2 plants
  • The interaction between OsPHO2 and OsGI links high-level regulators of Pi homeostasis and development in rice
  • A yeast-two-hybrid screen using Oryza sativa (rice) PHO2 as bait, revealed an interaction between OsPHO2 and OsGIGANTEA, a key regulator of flowering time, which was confirmed using bimolecular flourescenec complementation (BiFC)
  • Pi analysis of individual leaves demonstrated that Osgi, similar to Ospho2 mutants, were impaired in Pi remobilization from old to young leaves, albeit to a lesser extent
  • Finally, the dynamic transcriptional regulation of OsRNS genes by overexpression of OsPHR2, ospho2 mutant, and overexpression of OsPT1 lines involved in Pi signaling pathway suggests the molecular basis of OsRNS family in Pi recycling via RNA decay under Pi starvation
  • The sap experiment clearly showed the significant increases in levels of Phe in the xylem sap of ospho2 than the WT grown hydroponically with Phe and +Pi
  • However, the concentrations of Phe in the seeds were comparable in the WT and mutants, suggesting a pivotal of OsPHO2 in attenuating Phe toxicity in the seed
  • Distribution of phenanthrene in the ospho2 reveals the involvement of phosphate on phenanthrene translocation and accumulation in rice.
  • In the present study, the role of OsPHO2 in regulating the translocation and accumulation of phenanthrene (Phe) and the involvement of Pi in this process were investigated
  • In +Phe WT, the relative expression level of OsPHO2 in the shoots was significantly lower, while those of Pi transporters (PTs) OsPT4 and OsPT8 were significantly higher in the roots compared with -Phe
• Connection
  • LTN1~OsPHO2~OsRLS1, OsPht1;2~OsPT2, Molecular mechanisms regulating Pi-signaling and Pi homeostasis under OsPHR2, a central Pi-signaling regulator, in rice, OsPHR2 positively regulates the low-affinity Pi transporter OsPT2 through physical interaction and reciprocal regulation of OsPHO2 in roots
  • LTN1~OsPHO2~OsRLS1, OsPHR2, Molecular mechanisms regulating Pi-signaling and Pi homeostasis under OsPHR2, a central Pi-signaling regulator, in rice, OsPHR2 positively regulates the low-affinity Pi transporter OsPT2 through physical interaction and reciprocal regulation of OsPHO2 in roots
  • LTN1~OsPHO2~OsRLS1, OsPHR2, OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, Here we report that OsPHR2 positively regulates the low-affinity Pi transporter gene OsPT2 by physical interaction and upstream regulation of OsPHO2 in roots
  • LTN1~OsPHO2~OsRLS1, OsPHR2, OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, OsSPX1 suppresses the regulation on expression of OsPT2 by OsPHR2 and the accumulation of excess shoot Pi, but it does not suppress induction of OsPT2 or the accumulation of excessive shoot Pi in the Ospho2 mutant
  • LTN1~OsPHO2~OsRLS1, OsPHR2, OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, Our data also show that OsSPX1 is a negative regulator of OsPHR2 and is involved in the feedback of Pi-signaling network in roots that is defined by OsPHR2 and OsPHO2
  • LTN1~OsPHO2~OsRLS1, OsSPX1, OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, OsSPX1 suppresses the regulation on expression of OsPT2 by OsPHR2 and the accumulation of excess shoot Pi, but it does not suppress induction of OsPT2 or the accumulation of excessive shoot Pi in the Ospho2 mutant
  • LTN1~OsPHO2~OsRLS1, OsSPX1, OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, Our data also show that OsSPX1 is a negative regulator of OsPHR2 and is involved in the feedback of Pi-signaling network in roots that is defined by OsPHR2 and OsPHO2
  • LTN1~OsPHO2~OsRLS1, OsPht1;2~OsPT2, OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, Here we report that OsPHR2 positively regulates the low-affinity Pi transporter gene OsPT2 by physical interaction and upstream regulation of OsPHO2 in roots
  • LTN1~OsPHO2~OsRLS1, OsPht1;2~OsPT2, OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice, OsSPX1 suppresses the regulation on expression of OsPT2 by OsPHR2 and the accumulation of excess shoot Pi, but it does not suppress induction of OsPT2 or the accumulation of excessive shoot Pi in the Ospho2 mutant
  • LTN1~OsPHO2~OsRLS1, OsPHR2, Involvement of OsSPX1 in phosphate homeostasis in rice, Suppression of OsSPX1 by RNA interference resulted in severe signs of toxicity caused by the over-accumulation of Pi, similar to that found in OsPHR2 (phosphate starvation response transcription factor 2) overexpressors and pho2 (phosphate-responsive mutant 2)
  • LTN1~OsPHO2~OsRLS1, OsPHR2, Involvement of OsSPX1 in phosphate homeostasis in rice, Quantitative RT-PCR showed that expression of OsSPX1 was strongly induced in OsPHR2 overexpression and pho2 mutant plants, indicating that OsSPX1 occurs downstream of OsPHR2 and PHO2
  • LTN1~OsPHO2~OsRLS1, OsSPX1, Involvement of OsSPX1 in phosphate homeostasis in rice, Suppression of OsSPX1 by RNA interference resulted in severe signs of toxicity caused by the over-accumulation of Pi, similar to that found in OsPHR2 (phosphate starvation response transcription factor 2) overexpressors and pho2 (phosphate-responsive mutant 2)
  • LTN1~OsPHO2~OsRLS1, OsSPX1, Involvement of OsSPX1 in phosphate homeostasis in rice, Quantitative RT-PCR showed that expression of OsSPX1 was strongly induced in OsPHR2 overexpression and pho2 mutant plants, indicating that OsSPX1 occurs downstream of OsPHR2 and PHO2
  • LTN1~OsPHO2~OsRLS1, OsSPX3, Regulation of OsSPX1 and OsSPX3 on expression of OsSPX domain genes and Pi-starvation signaling in rice, OsSPX3 negatively regulates the PSI (Pi-starvation induced) gene, OsIPS1 and is involved in the responses of miR399 and OsPHO2 to Pi-starvation
  • LTN1~OsPHO2~OsRLS1, OsSPX3, Regulation of OsSPX1 and OsSPX3 on expression of OsSPX domain genes and Pi-starvation signaling in rice, OsSPX3 plays a role in OsIPS1/miR399 mediated long distance regulation on OsPHO2
  • LTN1~OsPHO2~OsRLS1, OsSPX1, Down-regulation of OsSPX1 causes high sensitivity to cold and oxidative stresses in rice seedlings, OsPHO2) were significantly down-regulated by the antisense of OsSPX1
  • LTN1~OsPHO2~OsRLS1, OsARF12, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • LTN1~OsPHO2~OsRLS1, OsSPX-MFS1~OsPSS1, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • LTN1~OsPHO2~OsRLS1, OsSPX-MFS2, Auxin response factor OsARF12, a novel regulator for phosphate homeostasis in rice Oryza sativa, Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPX-MFS2
  • LTN1~OsPHO2~OsRLS1, OsPht1;1~OsPT1, A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-replete rice, Furthermore, OsPT1 expression was strongly enhanced by the mutation of Phosphate Overaccumulator2 (OsPHO2) but not by Phosphate Starvation Response2, indicating that OsPT1 is involved in the OsPHO2-regulated Pi pathway
  • LTN1~OsPHO2~OsRLS1, OsLPR3, Identification and expression analysis of OsLPR family revealed the potential roles of OsLPR3 and 5 in maintaining phosphate homeostasis in rice., Further, the expression levels of OsLPR3 and 5 were either attenuated in ossiz1 and ospho2 or augmented in rice overexpressing OsSPX1
  • LTN1~OsPHO2~OsRLS1, OsTrxh4, Two h-type thioredoxins interact with the PHO2ubiquitin-conjugating E2 enzyme to fine-tune phosphate homeostasis., A yeast-two-hybrid (Y2H) screen in Oryza sativa (Os, rice) using OsPHO2 as bait, revealed an interaction between OsPHO2 and two h-type thioredoxins, OsTrxh1 and OsTrxh4
  • LTN1~OsPHO2~OsRLS1, OsTrx23~OsTRXh1, Two h-Type Thioredoxins Interact with the E2 Ubiquitin Conjugase PHO2 to Fine-Tune Phosphate Homeostasis in Rice., A yeast two-hybrid (Y2H) screen in rice (Oryza sativa; Os) using OsPHO2 as bait revealed an interaction between OsPHO2 and two h-type thioredoxins, OsTrxh1 and OsTrxh4
  • LTN1~OsPHO2~OsRLS1, OsTrx23~OsTRXh1, Two h-Type Thioredoxins Interact with the E2 Ubiquitin Conjugase PHO2 to Fine-Tune Phosphate Homeostasis in Rice., Characterization of rice pho2 complemented lines, transformed with an endogenous genomic OsPHO2 or OsPHO2(C445S) (a constitutively reduced form) fragment, indicated that OsPHO2(C445S) restored Pi concentration in rice to statistically significant lower levels compared to native OsPHO2 Moreover, the suppression of OsTrxh1 (knockdown and knockout) resulted in slightly higher Pi concentration than that of wild-type Nipponbare in leaves
  • LTN1~OsPHO2~OsRLS1, OsTrxh4, Two h-Type Thioredoxins Interact with the E2 Ubiquitin Conjugase PHO2 to Fine-Tune Phosphate Homeostasis in Rice., A yeast two-hybrid (Y2H) screen in rice (Oryza sativa; Os) using OsPHO2 as bait revealed an interaction between OsPHO2 and two h-type thioredoxins, OsTrxh1 and OsTrxh4
  • LTN1~OsPHO2~OsRLS1, OsNLA1, OsNLA1, a RING-type ubiquitin ligase, maintains phosphate homeostasis in Oryza sativa via degradation of phosphate transporters., Moreover, there was no interaction of OsNLA1 and OsPHO2, an E2 ubiquitin-conjugase, suggesting that OsPHO2 was not the partner of OsNLA1 involved in ubiquitin-mediated PT degradation
  • LTN1~OsPHO2~OsRLS1, OsGI, Molecular interaction between PHO2 and GI in rice., Characterization of rice Osgi and Ospho2 mutants revealed that they displayed several similar phenotypic features supporting a physiological role for this interaction
  • LTN1~OsPHO2~OsRLS1, OsGI, Molecular interaction between PHO2 and GI in rice., Reduced growth, leaf tip necrosis, delayed flowering and over-accumulation of Pi in leaves compared to wild-type were shared features of Osgi and Ospho2 plants
  • LTN1~OsPHO2~OsRLS1, OsGI, Molecular interaction between PHO2 and GI in rice., Pi analysis of individual leaves demonstrated that Osgi, similar to Ospho2 mutants, were impaired in Pi remobilization from old to young leaves, albeit to a lesser extent
  • LTN1~OsPHO2~OsRLS1, OsGI, Molecular interaction between PHO2 and GI in rice., Transcriptome analyses revealed more than 55% of the genes differentially expressed in Osgi plants overlapped with the set of differentially expressed genes in Ospho2 plants
  • LTN1~OsPHO2~OsRLS1, OsGI, Molecular interaction between PHO2 and GI in rice., The interaction between OsPHO2 and OsGI links high-level regulators of Pi homeostasis and development in rice
  • LTN1~OsPHO2~OsRLS1, Pho2, Molecular interaction between PHO2 and GI in rice., A yeast-two-hybrid screen using Oryza sativa (rice) PHO2 as bait, revealed an interaction between OsPHO2 and OsGIGANTEA, a key regulator of flowering time, which was confirmed using bimolecular flourescenec complementation (BiFC)
  • LTN1~OsPHO2~OsRLS1, OsNLA1, Altered Expression of OsNLA1 Modulates Pi Accumulation in Rice Oryza sativa L. Plants., Moreover, OsNLA1 was also found to interact with OsPHO2, a known regulator of Pi homeostasis, in a Yeast Two-Hybrid (Y2H) assay
  • LTN1~OsPHO2~OsRLS1, OsPHR2, Phosphate-Starvation-Inducible S-Like RNase Genes in Rice Are Involved in Phosphate Source Recycling by RNA Decay, Finally, the dynamic transcriptional regulation of OsRNS genes by overexpression of OsPHR2, ospho2 mutant, and overexpression of OsPT1 lines involved in Pi signaling pathway suggests the molecular basis of OsRNS family in Pi recycling via RNA decay under Pi starvation
  • LTN1~OsPHO2~OsRLS1, OsPht1;1~OsPT1, Phosphate-Starvation-Inducible S-Like RNase Genes in Rice Are Involved in Phosphate Source Recycling by RNA Decay, Finally, the dynamic transcriptional regulation of OsRNS genes by overexpression of OsPHR2, ospho2 mutant, and overexpression of OsPT1 lines involved in Pi signaling pathway suggests the molecular basis of OsRNS family in Pi recycling via RNA decay under Pi starvation
  • LTN1~OsPHO2~OsRLS1, OsPP95, PROTEIN PHOSPHATASE95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice[OPEN], We show that OsPHO2 interacts with and induces the degradation of OsPP95
  • LTN1~OsPHO2~OsRLS1, OsPP95, PROTEIN PHOSPHATASE95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice[OPEN], We conclude that OsPP95, a protein phosphatase negatively regulated by OsPHO2, positively regulates Pi homeostasis and remobilization by dephosphorylating PTs and affecting their trafficking to the PM, a reversible process required for adaptation to variable Pi conditions
  • LTN1~OsPHO2~OsRLS1, OsPht1;4~OsPT4, Distribution of phenanthrene in the ospho2 reveals the involvement of phosphate on phenanthrene translocation and accumulation in rice., In +Phe WT, the relative expression level of OsPHO2 in the shoots was significantly lower, while those of Pi transporters (PTs) OsPT4 and OsPT8 were significantly higher in the roots compared with -Phe
  • LTN1~OsPHO2~OsRLS1, OsPht1;8~OsPT8, Distribution of phenanthrene in the ospho2 reveals the involvement of phosphate on phenanthrene translocation and accumulation in rice., In +Phe WT, the relative expression level of OsPHO2 in the shoots was significantly lower, while those of Pi transporters (PTs) OsPT4 and OsPT8 were significantly higher in the roots compared with -Phe

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