OsRad21-4,OsREC8

2015-01-20 | Categories genes  | Tags meiosis  meiotic 

• Information
  • Symbol: OsRad21-4,OsREC8
  • MSU: LOC_Os05g50410
  • RAPdb: Os05g0580500
• Publication
  • OsAM1 is required for leptotene-zygotene transition in rice, 2011, Cell Res.
  • MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, 2009, J Cell Sci.
  • OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, 2011, Plant J.
  • The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, 2012, Plant J.
  • OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, 2011, Plant Physiol.
  • OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice, 2010, Chromosoma.
  • The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis, 2006, Plant Mol Biol.
  • Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells, 2020, Front Plant Sci ..
• Genbank accession number
  • AY371049
• Key message
  • These observations suggest that OsRad21-4 is essential for efficient meiosis
  • The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis
  • Furthermore, fluorescent in situ hybridization experiments on Osrec8 meiocytes demonstrated that the mutation eliminated meiotic centromeric cohesion completely during prophase I and also led to the bipolar orientation of the kinetochores during the first meiotic division and accordingly resulted in premature separation of sister chromatid during meiosis I
  • OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis
  • We showed that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I
  • These results suggest that OsREC8 has several essential roles in the meiotic processes
  • Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • Very faint OsREC8 foci persisted in the Osam1 mutant, indicating that OsAM1 is not required for the initial meiotic recruitment of OsREC8
  • Meiotic chromosome behaviors were monitored from male meiocytes of OsRAD21-4-deficient lines mediated by RNAi
  • Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
• Connection
  • OsRad21-4~OsREC8, PAIR2, OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice, Although the two axial-associated proteins, REC8 and PAIR2, are loaded onto the chromosomes, the depletion of PAIR2 from the chromosomes is much later than in wild type
  • OsAM1, OsRad21-4~OsREC8, OsAM1 is required for leptotene-zygotene transition in rice, Very faint OsREC8 foci persisted in the Osam1 mutant, indicating that OsAM1 is not required for the initial meiotic recruitment of OsREC8
  • OsRad21-4~OsREC8, PAIR2, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, In addition, MER3 does not colocalize with PAIR2 at the beginning of prophase I, but locates on one end of PAIR2 fragments at later stages, whereas MER3 foci merely locate on one end of REC8 fragments when signals start to be seen in early prophase I
  • OsRad21-4~OsREC8, PAIR2, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, The normal loading of PAIR2 and REC8 in mer3 implies that their loading is independent of MER3
  • MER3~RCK, OsRad21-4~OsREC8, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, In addition, MER3 does not colocalize with PAIR2 at the beginning of prophase I, but locates on one end of PAIR2 fragments at later stages, whereas MER3 foci merely locate on one end of REC8 fragments when signals start to be seen in early prophase I
  • MER3~RCK, OsRad21-4~OsREC8, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, The normal loading of PAIR2 and REC8 in mer3 implies that their loading is independent of MER3
  • MER3~RCK, OsRad21-4~OsREC8, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsAM1, OsRad21-4~OsREC8, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsRad21-4~OsREC8, ZEP1~OsZEP, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsRad21-4~OsREC8, OsSGO1, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsRad21-4~OsREC8, PAIR2, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsCOM1, OsRad21-4~OsREC8, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • OsCOM1, OsRad21-4~OsREC8, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsRad21-4~OsREC8, PAIR3, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • OsRad21-4~OsREC8, OsSPO11-1~OsTOP6A1, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • MER3~RCK, OsRad21-4~OsREC8, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • MER3~RCK, OsRad21-4~OsREC8, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsRad21-4~OsREC8, ZEP1~OsZEP, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • OsRad21-4~OsREC8, ZEP1~OsZEP, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsRad21-4~OsREC8, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • OsRad21-4~OsREC8, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • MER3~RCK, OsRad21-4~OsREC8, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8
  • MER3~RCK, OsRad21-4~OsREC8, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins
  • OsRad21-4~OsREC8, PAIR2, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8
  • OsRad21-4~OsREC8, PAIR2, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins
  • OsRad21-4~OsREC8, ZEP1~OsZEP, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8
  • OsRad21-4~OsREC8, ZEP1~OsZEP, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins
  • OsMTOPVIB, OsRad21-4~OsREC8, OsMTOPVIB is required for meiotic bipolar spindle assembly., We further revealed that the mono-orientation of sister kinetochores was prevalent in OsmtopVIB, whereas biorientation of sister kinetochores was frequently observed in Osspo11-1, pair2, and crc1 In addition, mutations of the cohesion subunit OsREC8 resulted in biorientation of sister kinetochores as well as bipolar spindles even in the background of OsmtopVIB Therefore, we propose that biorientation of the kinetochore is required for bipolar spindle assembly in the absence of homologous recombination

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