OsLEA5

2015-01-20 | Categories genes  | Tags panicle  root  salinity  abiotic stress  transcription factor  seed  seed germination  ABA  zinc  ABA  seedlings  defense  drought  salt  tolerance  salt stress  stress  stress response  quality  endosperm  seed weight 

• Information
  • Symbol: OsLEA5
  • MSU: LOC_Os05g50710
  • RAPdb: Os05g0584200
• Publication
  • Molecular characterization and functional analysis by heterologous expression in E. coli under diverse abiotic stresses for OsLEA5, the atypical hydrophobic LEA protein from Oryza sativa L, 2012, Mol Genet Genomics.
  • The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., 2017, Biochem Biophys Res Commun.
  • An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., 2018, Plant Cell Physiol.
  • Overexpression of rice OsLEA5 relieves the deterioration in seed quality caused by high-temperature stress., 2020, Plant Biotechnol (Tokyo).
• Genbank accession number
  • JF776156
• Key message
  • The expression levels of OsLEA5 in the roots and panicles of full ripe stage were dramatically increased
  • coli cells producing OsLEA5 fusion protein exhibited improved resistance against diverse abiotic stresses: high salinity, osmotic, freezing, heat, and UV radiation
  • The OsLEA5 protein confers stabilization of the LDH under different abiotic stresses, such as heating, freeze-thawing, and drying in vitro
  • The combined results indicated that OsLEA5 protein was a hydrophobic atypical LEA and closely associated with resistance to multiple abiotic stresses
  • Molecular characterization and functional analysis by heterologous expression in E. coli under diverse abiotic stresses for OsLEA5, the atypical hydrophobic LEA protein from Oryza sativa L
  • The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice.
  • The seed germination is highly inhibited in ZFP36 overexpression plants under ABA treatment, while an RNA interference (RNAi) mutant of OsLEA5 rice seeds were less sensitive to ABA, and exogenous ASC (ascorbate acid) could alleviate the inhibition induced by ABA
  • Thus, our conclusion is that OsAPX1 is a direct target of ZFP36 and OsLEA5 could interact with ZFP36 to co-regulate ABA-inhibited seed germination by controlling the expression of OsAPX1
  • Hence, our conclusion is that OsLEA5 participates in the ABA-mediated antioxidant defense to function in drought and salt stress response in rice, and 5C subgroup of LEAs contribute to play co-regulators of the transcription factor ZFP36
  • OsLEA5 acts as a co-regulator of a transcriptional factor ZFP36 to enhance the expression and the activity of ascorbate peroxidase OsAPX1 to regulate seed germination in rice, but it it unknown whether OsLEA5 is also crucial in plant seedlings under stress conditions
  • An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L.
  • Further investigation found that various stimulus and ABA could induce OsLEA5 expression, and OsLEA5 played downstream of ZFP36 to be involved in ABA-induced generation of hydrogen peroxide (H2O2), and the regulation of the expression and the activities of antioxidant defense enzymes in plants leaves, and OsLEA5 contributed to stabilize ZFP36
  • We found that overexpression of OsLEA5 in rice plants enhanced the tolerance to drought and salt stress; in contrast, an RNA interference (RNAi) mutant of OsLEA5 rice plants were more sensitive to drought and salinity
  • Additionally, OsLEA5 participates in the accumulation of ABA by upregulating ABA-biosynthesis genes and downregulating ABA-metabolism genes
  • Moreover, we found two homologue of OsLEA5 (5C700, short for Os05g0526700 and 5C300, short for Os05g0584300) which were induced by ABA also interacted with ZFP36 separately; interestingly, the nucleus-located 5C700 could also act as a co-activator of ZFP36 to modulate OsAPX1 while 5C300 which was ABA-induced downexpressed played as an ABA-induced inhibitor of ZFP36 to regulate OsAPX1
  • Overexpression of rice OsLEA5 relieves the deterioration in seed quality caused by high-temperature stress.
  • RNAi knockdown line of OsLEA5 showed decreased seed weight
  • Transformant lines overexpressing OsLEA5 exhibited improved quality and seed weight of mature seeds when they were developed under high-temperature conditions, while seed quality strongly declined in wild-type plants exposed to high-temperature stress
  • These findings indicate that OsLEA5 contributes to suppressing the deterioration of seed quality when developed under high-temperature conditions
  • OsLEA5 (Lea14A) interacts with a regulator of the endosperm storage production, FLO2, suggesting that OsLEA5 may be involved in endosperm quality control
• Connection
  • BSR-D1~ZFP36, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice.
  • BSR-D1~ZFP36, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Moreover, ZFP36 could interact with a late embryogenesis abundant protein OsLEA5 to co-regulate the promoter activity of OsAPX1
  • BSR-D1~ZFP36, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., The seed germination is highly inhibited in ZFP36 overexpression plants under ABA treatment, while an RNA interference (RNAi) mutant of OsLEA5 rice seeds were less sensitive to ABA, and exogenous ASC (ascorbate acid) could alleviate the inhibition induced by ABA
  • BSR-D1~ZFP36, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Thus, our conclusion is that OsAPX1 is a direct target of ZFP36 and OsLEA5 could interact with ZFP36 to co-regulate ABA-inhibited seed germination by controlling the expression of OsAPX1
  • OsAPX1~APXa, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice.
  • OsAPX1~APXa, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Moreover, ZFP36 could interact with a late embryogenesis abundant protein OsLEA5 to co-regulate the promoter activity of OsAPX1
  • OsAPX1~APXa, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Thus, our conclusion is that OsAPX1 is a direct target of ZFP36 and OsLEA5 could interact with ZFP36 to co-regulate ABA-inhibited seed germination by controlling the expression of OsAPX1
  • BSR-D1~ZFP36, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., OsLEA5 acts as a co-regulator of a transcriptional factor ZFP36 to enhance the expression and the activity of ascorbate peroxidase OsAPX1 to regulate seed germination in rice, but it it unknown whether OsLEA5 is also crucial in plant seedlings under stress conditions
  • BSR-D1~ZFP36, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., Further investigation found that various stimulus and ABA could induce OsLEA5 expression, and OsLEA5 played downstream of ZFP36 to be involved in ABA-induced generation of hydrogen peroxide (H2O2), and the regulation of the expression and the activities of antioxidant defense enzymes in plants leaves, and OsLEA5 contributed to stabilize ZFP36
  • BSR-D1~ZFP36, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., Moreover, we found two homologue of OsLEA5 (5C700, short for Os05g0526700 and 5C300, short for Os05g0584300) which were induced by ABA also interacted with ZFP36 separately; interestingly, the nucleus-located 5C700 could also act as a co-activator of ZFP36 to modulate OsAPX1 while 5C300 which was ABA-induced downexpressed played as an ABA-induced inhibitor of ZFP36 to regulate OsAPX1
  • BSR-D1~ZFP36, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., Hence, our conclusion is that OsLEA5 participates in the ABA-mediated antioxidant defense to function in drought and salt stress response in rice, and 5C subgroup of LEAs contribute to play co-regulators of the transcription factor ZFP36
  • OsAPX1~APXa, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., OsLEA5 acts as a co-regulator of a transcriptional factor ZFP36 to enhance the expression and the activity of ascorbate peroxidase OsAPX1 to regulate seed germination in rice, but it it unknown whether OsLEA5 is also crucial in plant seedlings under stress conditions
  • OsAPX1~APXa, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., Moreover, we found two homologue of OsLEA5 (5C700, short for Os05g0526700 and 5C300, short for Os05g0584300) which were induced by ABA also interacted with ZFP36 separately; interestingly, the nucleus-located 5C700 could also act as a co-activator of ZFP36 to modulate OsAPX1 while 5C300 which was ABA-induced downexpressed played as an ABA-induced inhibitor of ZFP36 to regulate OsAPX1
  • FLO2, OsLEA5, Overexpression of rice OsLEA5 relieves the deterioration in seed quality caused by high-temperature stress., OsLEA5 (Lea14A) interacts with a regulator of the endosperm storage production, FLO2, suggesting that OsLEA5 may be involved in endosperm quality control

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