OsPP2C53,OsABIL2

2015-01-20 | Categories genes | Tags ABA stomatal ABA stomata water loss root development drought root development stress drought stress nucleus root architecture plant development Kinase root hair protein kinase

Information
- Symbol: OsPP2C53,OsABIL2
- MSU: LOC_Os05g51510
- RAPdb: Os05g0592800
Publication
Genbank accession number
Key message
- OsPP2C50 and OsPP2C53 is major negative regulators of ABA signaling regarding stomata closing in rice
- We identified that only OsPP2C50 and OsPP2C53 among 9 OsPP2CAs might be related with stomatal closure/opening signaling based on guard cell specific expression and subcellular localization
- Transgenic rice overexpressing OsPP2C50 and OsPP2C53 showed significantly higher water loss than control
- Overexpression of OsABIL2 not only led to the ABA insensitivity, but also significantly altered plant developmental phenotypes, including stomata density and root architecture, which likely caused the hypersensitivity to drought stress
- Taken together, this study provides significant insights into rice ABA signaling and indicates the important role of OsABIL2 in regulating root development
- Furthermore, OsABIL2 interacts with and co-localized with OsPYL1 mainly in the cytosol, and ABA treatment regulates the nucleus-cytosol distribution of OsABIL2, suggesting a different mechanism for the activation of ABA signaling
- However, unlike many other solely nucleus-localized clade A PP2Cs, the OsABIL2 is localized in both nucleus and cytosol
- Transgenic rice overexpressing SAPK10 (Stress/ABA-activated protein kinase 10) had longer root hairs; rice plants overexpressing OsABIL2 (OsABI-Like 2) had attenuated ABA signaling and shorter root hairs, suggesting that the effect of ABA on root hair elongation depends on the conserved PYR/PP2C/SnRK2 ABA signaling module
- A yeast two-hybrid assay revealed that the interaction between OsABIL2 and a putative rice ABA receptor, OsPYL1, was ABA-dependent, and the interaction was lost with amino acid substitution from glycine to aspartic acid at the 183rd amino acid of the OsABIL2 protein, corresponding to abi1-1 mutation
- The constitutive expression of OsABIL2 or OsABIL2G183D in Arabidopsis or rice decreased ABA sensitivity to differing degrees
- These results indicated that the introduction of abi1-1 type dominant mutation was also effective in OsABIL2 at decreasing ABA sensitivity in plants, and the attenuation of ABA sensitivity could be an alternative parameter to improve rice performance under low temperatures
Connection
- OsABIL3~OsPP2C50, OsPP2C53~OsABIL2, Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice., We identified that only OsPP2C50 and OsPP2C53 among 9 OsPP2CAs might be related with stomatal closure/opening signaling based on guard cell specific expression and subcellular localization
- OsABIL3~OsPP2C50, OsPP2C53~OsABIL2, Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice., Transgenic rice overexpressing OsPP2C50 and OsPP2C53 showed significantly higher water loss than control
- OsABIL3~OsPP2C50, OsPP2C53~OsABIL2, Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice., We also characterized the interaction networks between OsPP2C50 and OsPP2C53, SAPK10 and OsSLAC1 and found two interaction pathways among those signaling components: a hierarchical interaction pathway that consisted of OsPP2C50 and OsPP2C53, SAPK10 and OsSLAC1; and a branched interaction pathway wherein OsPP2C50 and OsPP2C53 interacted directly with OsSLAC1
- OsABIL3~OsPP2C50, OsPP2C53~OsABIL2, Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice., OsPP2C50 and OsPP2C53 is major negative regulators of ABA signaling regarding stomata closing in rice
- OsBIABP1~SLAC1~OsSLAC1, OsPP2C53~OsABIL2, Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice., We also characterized the interaction networks between OsPP2C50 and OsPP2C53, SAPK10 and OsSLAC1 and found two interaction pathways among those signaling components: a hierarchical interaction pathway that consisted of OsPP2C50 and OsPP2C53, SAPK10 and OsSLAC1; and a branched interaction pathway wherein OsPP2C50 and OsPP2C53 interacted directly with OsSLAC1
- OsPP2C53~OsABIL2, SAPK10, Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice., We also characterized the interaction networks between OsPP2C50 and OsPP2C53, SAPK10 and OsSLAC1 and found two interaction pathways among those signaling components: a hierarchical interaction pathway that consisted of OsPP2C50 and OsPP2C53, SAPK10 and OsSLAC1; and a branched interaction pathway wherein OsPP2C50 and OsPP2C53 interacted directly with OsSLAC1
- OsPP2C53~OsABIL2, OsSAPK8~SAPK8, Abscisic acid regulates subcellular redistribution of OsABI-LIKE2, a negative regulator in ABA signaling, to control root architecture and drought resistance in Oryza Sativa., OsABIL2 interacts with OsPYL1, SAPK8 and SAPK10 both in vitro and in vivo, and the phosphatase activity of OsABIL2 was repressed by ABA-bound OsPYL1
- OsPP2C53~OsABIL2, SAPK10, Abscisic acid regulates subcellular redistribution of OsABI-LIKE2, a negative regulator in ABA signaling, to control root architecture and drought resistance in Oryza Sativa., OsABIL2 interacts with OsPYL1, SAPK8 and SAPK10 both in vitro and in vivo, and the phosphatase activity of OsABIL2 was repressed by ABA-bound OsPYL1
- OsPP2C53~OsABIL2, OsPYL1, Abscisic acid regulates subcellular redistribution of OsABI-LIKE2, a negative regulator in ABA signaling, to control root architecture and drought resistance in Oryza Sativa, OsABIL2 interacts with OsPYL1, SAPK8 and SAPK10 both in vitro and in vivo, and the phosphatase activity of OsABIL2 was repressed by ABA-bound OsPYL1
- OsPP2C53~OsABIL2, SAPK10, Abscisic Acid Regulates Auxin Homeostasis in Rice Root Tips to Promote Root Hair Elongation., Transgenic rice overexpressing SAPK10 (Stress/ABA-activated protein kinase 10) had longer root hairs; rice plants overexpressing OsABIL2 (OsABI-Like 2) had attenuated ABA signaling and shorter root hairs, suggesting that the effect of ABA on root hair elongation depends on the conserved PYR/PP2C/SnRK2 ABA signaling module
- OsPP2C53~OsABIL2, OsPYL1, Ectopic expression of mutated type 2C protein phosphatase OsABI-LIKE2 decreases abscisic acid sensitivity in Arabidopsis and rice., A yeast two-hybrid assay revealed that the interaction between OsABIL2 and a putative rice ABA receptor, OsPYL1, was ABA-dependent, and the interaction was lost with amino acid substitution from glycine to aspartic acid at the 183rd amino acid of the OsABIL2 protein, corresponding to abi1-1 mutation
- HDA704, OsPP2C53~OsABIL2, Rice histone deacetylase HDA704 positively regulates drought and salt tolerance by controlling stomatal aperture and density, Our results showed that HDA704 directly binds to DST and ABIL2, repressing their expression via histone deacetylation modification.
- GORI~OsABT, OsPP2C53~OsABIL2, OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage., The yeast two-hybrid and bimolecular fluorescence complementation analyses showed that OsABT interacted with the ABA receptor proteins OsPYL4, OsPYL10, and PP2C phosphatase OsABIL2
- OsPP2C53~OsABIL2, OsPYL10, OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage., The yeast two-hybrid and bimolecular fluorescence complementation analyses showed that OsABT interacted with the ABA receptor proteins OsPYL4, OsPYL10, and PP2C phosphatase OsABIL2

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