| Categories genes  | Tags chloroplast  development  map-based cloning  chloroplast development 
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  • Key message
    • By transient expression in Nicotiana benthamiana, we found that RA1 protein was localized in the chloroplast
    • RNA analysis revealed biogenesis of chloroplast rRNAs was abnormal in ra1
    • These results suggest that RA1 is involved in early chloroplast development and establishment of the plastidic ribosome system in rice
    • Expression levels of chlorophyll biosynthesis and plastid development related genes were disordered in the ra1 mutant
    • Map-based cloning revealed that RA1 gene encodes a glycyl-tRNA synthetase (GlyRS), which was confirmed by genetic complementation and knockout by Crispr/Cas9 technology
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