- Information
- Symbol: EPSPs,OsEPSPS
- MSU: LOC_Os06g04280
- RAPdb: Os06g0133900
- Publication
- Evidence for an evolutionary force that prevents epigenetic silencing between tail-to-tail rice genes with a short spacer, 2005, Gene.
- Gene replacements and insertions in rice by intron targeting using CRISPR-Cas9., 2016, Nat Plants.
- Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice., 2020, Plant Biotechnol J..
- CRISPR/Cas9-mediated homology donor repair base editing confers glyphosate resistance to rice Oryza sativa L.., 2023, Front Plant Sci.
- Genbank accession number
- Key message
- Here, we describe the development of glyphosate-resistant rice lines by site-specific amino acid substitutions (G172A, T173I, and P177S: GATIPS-mOsEPSPS) and modification of phosphoenolpyruvate-binding site in the native OsEPSPS gene employing fragment knockout and knock-in of homology donor repair (HDR) template harboring desired mutations through CRISPR-Cas9-based genome editing
- Together, results highlighted that the efficacy of GATIPS mutations in OsEPSPS has tremendously contributed in glyphosate resistance (foliar spray of 6 ml/L), enhanced aromatic amino acids, and improved grain yields in rice
- Connection
Prev Next