OsMKK1,OsMEK2

• Symbol: OsMKK1,OsMEK2
• MSU: LOC_Os06g05520
• RAPdb: Os06g0147800

• The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice., 2014, Plant Sci.
• Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses, 2021, Plant Cell.
• Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions, 2021, Front Plant Sci.
• Rice iron storage protein ferritin 2 OsFER2 positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae., 2022, Front Plant Sci.

Genbank accession number

• By comparing the transcription factors levels between WT and osmkk1 mutant, OsMKK1 was necessary for salt-induced increase in OsDREB2B and OsMYBS3
• OsMKK1 activity in the wild-type seedlings and protoplasts was increased by salt stress
• Taken together, the data suggest that OsMKK1 and OsMPK4 constitute a signaling pathway that regulates salt stress tolerance in rice
• Yeast two-hybrid and in vitro and in vivo kinase assays revealed that OsMKK1 targeted OsMPK4
• Further analyses revealed that OsDMI3-mediated phosphorylation of OsMKK1 positively regulates ABA responses in seed germination, root growth, and tolerance to both water stress and oxidative stress
• The activation of OsMKK1 and its downstream kinase OsMPK1 is dependent on Thr-25 phosphorylation of OsMKK1 in ABA signaling
• Our results indicate that OsMKK1 is a direct target of OsDMI3, and OsDMI3-mediated phosphorylation of OsMKK1 plays an important role in activating the MAPK cascade and ABA signaling
• These combined results suggest that OsMEK2 and OsMPK1 expression positively regulates iron- and ROS-dependent ferroptotic cell death, and blast disease (susceptibility)-related cell death was ROS-dependent but iron-independent in rice-M
• Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions
• OsFER2 knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe(3+)) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes OsPAL1, OsPR1-b, OsRbohB, OsNADP-ME2-3, OsMEK2 and OsMPK1, and vacuolar membrane transporter OsVIT2 expression

• MYBS3~OsMYBS3, OsMKK1~OsMEK2, The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice., By comparing the transcription factors levels between WT and osmkk1 mutant, OsMKK1 was necessary for salt-induced increase in OsDREB2B and OsMYBS3
• OsDREB2B, OsMKK1~OsMEK2, The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice., By comparing the transcription factors levels between WT and osmkk1 mutant, OsMKK1 was necessary for salt-induced increase in OsDREB2B and OsMYBS3
• OsMKK1~OsMEK2, OsMPK6~OsMPK4, The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice., Yeast two-hybrid and in vitro and in vivo kinase assays revealed that OsMKK1 targeted OsMPK4
• OsMKK1~OsMEK2, OsMPK6~OsMPK4, The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice., OsMPK4 activity was increased by salt, which was impaired in osmkk1 plants
• OsMKK1~OsMEK2, OsMPK6~OsMPK4, The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice., In contrast, overexpression of OsMKK1 increased OsMPK4 activity in protoplasts
• OsMKK1~OsMEK2, OsMPK6~OsMPK4, The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice., Taken together, the data suggest that OsMKK1 and OsMPK4 constitute a signaling pathway that regulates salt stress tolerance in rice
• OsMAPKKK63, OsMKK1~OsMEK2, OsMAPKKK63 is involved in salt stress response and seed dormancy control., Our data indicate that OsMAPKKK63 possesses in vitro kinase activity and that it interacts with rice MAP kinase kinase OsMKK1 and OsMKK6
• OsCCaMK~OsDMI3, OsMKK1~OsMEK2, Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses, Here, we show that OsDMI3, a rice (Oryza sativa) CCaMK, directly interacts with and phosphorylates OsMKK1, a MAPK kinase (MKK) in rice, in vitro and in vivo
• OsCCaMK~OsDMI3, OsMKK1~OsMEK2, Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses, OsDMI3 was found to directly phosphorylate Thr-25 in the N-terminus of OsMKK1, and this Thr-25 phosphorylation is OsDMI3-specific in ABA signaling
• OsCCaMK~OsDMI3, OsMKK1~OsMEK2, Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses, Our results indicate that OsMKK1 is a direct target of OsDMI3, and OsDMI3-mediated phosphorylation of OsMKK1 plays an important role in activating the MAPK cascade and ABA signaling
• OsMKK1~OsMEK2, OsMPK1~OsMAPK6~OsSIPK, Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses, The activation of OsMKK1 and its downstream kinase OsMPK1 is dependent on Thr-25 phosphorylation of OsMKK1 in ABA signaling
• OsMKK1~OsMEK2, OsMPK1~OsMAPK6~OsSIPK, Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions, Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions
• OsMKK1~OsMEK2, OsMPK1~OsMAPK6~OsSIPK, Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions, Here, we show that rice MAP kinase (OsMEK2 and OsMPK1) signaling cascades are involved in iron- and ROS-dependent ferroptotic cell death responses of rice to M
• OsMKK1~OsMEK2, OsMPK1~OsMAPK6~OsSIPK, Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions, oryzae infection using OsMEK2 knock-out mutant and OsMEK2 and OsMPK1 overexpression rice plants
• OsMKK1~OsMEK2, OsMPK1~OsMAPK6~OsSIPK, Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions, These combined results suggest that OsMEK2 and OsMPK1 expression positively regulates iron- and ROS-dependent ferroptotic cell death, and blast disease (susceptibility)-related cell death was ROS-dependent but iron-independent in rice-M
• OsFER2, OsMKK1~OsMEK2, Rice iron storage protein ferritin 2 OsFER2 positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae., OsFER2 knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe(3+)) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes OsPAL1, OsPR1-b, OsRbohB, OsNADP-ME2-3, OsMEK2 and OsMPK1, and vacuolar membrane transporter OsVIT2 expression
• OsMKK1~OsMEK2, OsrbohB~Osrboh7, Rice iron storage protein ferritin 2 OsFER2 positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae., OsFER2 knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe(3+)) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes OsPAL1, OsPR1-b, OsRbohB, OsNADP-ME2-3, OsMEK2 and OsMPK1, and vacuolar membrane transporter OsVIT2 expression
• OsMKK1~OsMEK2, OsMPK1~OsMAPK6~OsSIPK, Rice iron storage protein ferritin 2 OsFER2 positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae., OsFER2 knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe(3+)) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes OsPAL1, OsPR1-b, OsRbohB, OsNADP-ME2-3, OsMEK2 and OsMPK1, and vacuolar membrane transporter OsVIT2 expression
• OsMKK1~OsMEK2, OsVIT2, Rice iron storage protein ferritin 2 OsFER2 positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae., OsFER2 knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe(3+)) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes OsPAL1, OsPR1-b, OsRbohB, OsNADP-ME2-3, OsMEK2 and OsMPK1, and vacuolar membrane transporter OsVIT2 expression
• OsMKK1~OsMEK2, PAL~OsPAL4~OsPAL1, Rice iron storage protein ferritin 2 OsFER2 positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae., OsFER2 knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe(3+)) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes OsPAL1, OsPR1-b, OsRbohB, OsNADP-ME2-3, OsMEK2 and OsMPK1, and vacuolar membrane transporter OsVIT2 expression