| Categories genes  | Tags transcription factor  temperature  endosperm  prolamin  demethylation 
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  • Key message
    • The expression of two transcription factor genes RISBZ1 and RPBF was significantly decreased in the osdml4 mutants, which caused adverse effects on the formation of protein bodies (PBs) with greatly decreased PB-II number, and incomplete and abnormally shaped PB-IIs
    • No significant difference was detected between the wild type and osdml4 mutants under normal temperature
    • Here, we identified a new demethylase gene OsDML4 and discovered its function in cytosine demethylation to affect endosperm formation
    • Loss of function of OsDML4 induced chalky endosperm only under HT and dramatically reduced the transcription and accumulation of glutelins and 16 kDa prolamin
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