OsOTS1

• Symbol: OsOTS1
• MSU: LOC_Os06g29310
• RAPdb: Os06g0487900

• SUMO is a critical regulator of salt stress responses in rice., 2016, Plant Physiol.
• Rice OVERLY TOLERANT TO SALT 1OTS1 SUMO protease is a positive regulator of seed germination and root development., 2016, Plant Signal Behav.
• Rice SUMO protease Overly Tolerant to Salt 1 targets the transcription factor, OsbZIP23 to promote drought tolerance in rice., 2017, Plant J.

Genbank accession number

• We demonstrate that OsOTS1 confers salt tolerance in rice by increasing root biomass
• High salinity triggers OsOTS1 degradation indicating that increased SUMO conjugation in rice plants during salt stress is in part achieved by down-regulation of OTS1/2 activity
• In this report, we reveal the Ubiquitin-like Protease (ULP) class of SUMO protease gene family in rice and demonstrate a critical role for OsOTS1 SUMO protease in salt stress
• Transgenic rice plants overexpressing OsOTS1 have increased salt tolerance and a concomitant reduction in the levels of SUMOylated proteins
• OsOTS1 is nuclear localised indicating a direct requirement of OsOTS1 dependent deSUMOylation activity in rice nuclei for salt tolerance
• Knockdown of OsOTS1 gene expression affects root growth by primarily reducing cell size rather than cell division
• We recently identified the SUMO protease gene family in rice and demonstrated a role for OsOTS1 SUMO proteases in salt stress
• We reveal that OsOTS1 SUMO protease directly targets the ABA and drought responsive transcription factor OsbZIP23 for de-SUMOylation affecting its stability
• In this study, we reveal the role of the SUMO protease, OsOTS1 in mediating tolerance to drought in rice
• OsOTS1 depleted transgenic plants accumulate more ABA and exhibit more productive agronomic traits during drought whilst OsOTS1 overexpressing lines are drought sensitive but ABA insensitive
• Drought and ABA treatment stimulates the degradation of OsOTS1 protein indicating that SUMO conjugation is an important response to drought stress in rice achieved through down-regulation of OTS1/2 activity
• OsOTS-RNAi lines show increased abundance of OsbZIP23 and increased drought responsive gene expression while OsOTS1 overexpressing lines show reduced levels of OsbZIP23 leading to suppressed drought responsive gene expression

• OsbZIP23, OsOTS1, Rice SUMO protease Overly Tolerant to Salt 1 targets the transcription factor, OsbZIP23 to promote drought tolerance in rice., We reveal that OsOTS1 SUMO protease directly targets the ABA and drought responsive transcription factor OsbZIP23 for de-SUMOylation affecting its stability
• OsbZIP23, OsOTS1, Rice SUMO protease Overly Tolerant to Salt 1 targets the transcription factor, OsbZIP23 to promote drought tolerance in rice., OsOTS-RNAi lines show increased abundance of OsbZIP23 and increased drought responsive gene expression while OsOTS1 overexpressing lines show reduced levels of OsbZIP23 leading to suppressed drought responsive gene expression