- Information
- Symbol: OsKOS1,OsKOL4,CYP701A8,qST-6.2
- MSU: LOC_Os06g37300
- RAPdb: Os06g0569500
- Publication
- A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, 2004, Plant Mol Biol.
- The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms, 2005, Plant Physiol.
- CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, 2012, Plant Physiol.
- Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis., 2015, Appl Microbiol Biotechnol.
- Assessment of efficacy of mutagenesis of gamma-irradiation in plant height and days to maturity through expression analysis in rice, 2021, PLoS One.
- Uncovering the Novel QTLs and Candidate Genes of Salt Tolerance in Rice with Linkage Mapping, RTM-GWAS, and RNA-seq., 2021, Rice (N Y).
- Genbank accession number
- Key message
- Introduction of OsKOL4 into d35Tan-Ginbozu did not rescue its dwarf phenotype, indicating that OsKOL4 is not involved in GA biosynthesis
- In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
- Thus, it appears that OsKOL4 plays a role in the more specialized diterpenoid metabolism of rice, and our results provide evidence for divergence of a KO/CYP701 family member from GA biosynthesis
- Gibberellin responsiveness study and quantitative real-time PCR showed a faulty gibberellin pathway and epistatic control between the genes such as OsKOL4 and OsBRD2 causing semi-dwarfism in a mutant
- Connection
- OsKOS1~OsKOL4~CYP701A8~qST-6.2, OsKOS2~OsKOL5, A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, Expression analysis revealed that OsKOL2 and OsKOL4 were actively transcribed in various organs, while OsKOL1 and OsKOL5 were expressed only at low levels; OsKOL3 may be a pseudogene
- OsKOS1~OsKOL4~CYP701A8~qST-6.2, OsKOS2~OsKOL5, A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, OsKOL4 and OsKOL5 are likely to take part in phytoalexin biosynthesis, because their expression was promoted by UV irradiation and/or elicitor treatment
- D35~OsKOS3~OsKO2, OsKOS1~OsKOL4~CYP701A8~qST-6.2, CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
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