- Information
- Symbol: D35,OsKOS3,OsKO2
- MSU: LOC_Os06g37364
- RAPdb: Os06g0570100
- Publication
- A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, 2004, Plant Mol Biol.
- Functional Identification of a Riceent-Kaurene Oxidase, OsKO2, Using thePichia pastorisExpression System, 2014, Bioscience, Biotechnology and Biochemistry.
- Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation, 2011, Plant Cell.
- The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms, 2005, Plant Physiol.
- CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, 2012, Plant Physiol.
- Overexpression of rice LRK1 restricts internode elongation by down-regulating OsKO2, 2013, Biotechnol Lett.
- Genbank accession number
- Key message
- ) semi-dwarf cultivar, Tan-Ginbozu (d35Tan-Ginbozu), contributed to the increase in crop productivity in Japan in the 1950s
- Previous studies suggested that the semi-dwarf stature of d35Tan-Ginbozu is caused by a defective early step of gibberellin biosynthesis, which is catalyzed by ent-kaurene oxidase (KO)
- Homozygote with null alleles of D35 showed a severe dwarf phenotype; therefore, d35Tan-Ginbozu is a weak allele of D35
- Introduction of OsKOL4 into d35Tan-Ginbozu did not rescue its dwarf phenotype, indicating that OsKOL4 is not involved in GA biosynthesis
- A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase
- Comparing d35Tan-Ginbozu with other high yielding cultivars, we discuss strategies to produce culm architectures suitable for high crop yield by decreasing GA levels
- Rice (Oryza sativa) contains five CYP701A subfamily members in its genome, despite the fact that only one (OsKO2/CYP701A6) is required for GA biosynthesis
- In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
- Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis
- This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice
- Connection
- D35~OsKOS3~OsKO2, OsKOS1~OsKOL4~CYP701A8~qST-6.2, CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
- D35~OsKOS3~OsKO2, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 encoding a NAC transcription factor affects plant height through mediating the gibberellic acid pathway in rice., Dual-luciferase reporter analyses, yeast one-hybrid assays and ChIP-qPCR suggested that OsNAC2 directly interacted with the promoter of OsEATB and OsKO2
- D35~OsKOS3~OsKO2, OsbZIP48, OsbZIP48, a HY5 transcription factor ortholog, exerts pleiotropic effects in light-regulated development., Electrophoretic mobility shift assay showed that OsbZIP48 directly binds to the OsKO2 promoter
- D35~OsKOS3~OsKO2, OsbHLH073, OsbHLH073 Negatively Regulates Internode Elongation and Plant Height by Modulating GA Homeostasis in Rice., We report that OsbHLH073 participates in regulating plant height, internode elongation, and panicle exsertion by regulating GA biosynthesis associated with the OsKO1 and OsKO2 genes
- D35~OsKOS3~OsKO2, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling, We also found that OsNAC2 regulates downstream targets in a time-dependent manner by binding to the promoter of OsKO2 in the seedling period but not in the germination stage
- D3, D35~OsKOS3~OsKO2, The miR528-D3 Module Regulates Plant Height in Rice by Modulating the Gibberellin and Abscisic Acid Metabolisms., Moreover, the expression level of GA biosynthesis-related key genes, including OsCPS1, OsCPS2, OsKO2 and OsKAO was remarkably higher in OE-D3 plants, while the NECD2 expression, a key gene involved in ABA biosynthesis, was significantly higher in d3 mutants than in WT and OE-D3 plants
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