- Information
- Symbol: OsLHY
- MSU: LOC_Os06g51260
- RAPdb: Os06g0728700
- Publication
- The evolutionarily conserved OsPRR quintet: rice pseudo-response regulators implicated in circadian rhythm, 2003, Plant Cell Physiol.
- The role of casein kinase II in flowering time regulation has diversified during evolution, 2010, Plant Physiol.
- Nitrogen Mediates Flowering Time and Nitrogen Use Efficiency via Floral Regulators in Rice, 2020, Curr Biol.
- Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis, 2020, EMBO J.
- Transcriptional and post-transcriptional regulation of heading date in rice, 2020, New Phytol.
- The transcriptional repressor OsPRR73 links circadian clock and photoperiod pathway to control heading date in rice, 2020, Plant Cell Environ.
- Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice, 2021, Plant Biotechnol J.
- mediated pathways in rice Oryza sativa L.., 2021, Plant Sci.
- Crosstalk between the Circadian Clock and Histone Methylation., 2022, Int J Mol Sci.
- Bifunctional regulators of photoperiodic flowering in short day plant rice., 2022, Front Plant Sci.
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Genbank accession number
- Key message
- OsCCA1 also regulates IPA1 expression to mediate panicle and grain development
- Downregulating and overexpressing OsCCA1 increases and reduces tiller numbers, respectively, whereas manipulating PSEUDORESPONSE REGULATOR1 (OsPPR1) expression results in the opposite effects
- Genetic analyses using double mutants and overexpression in the mutants show that OsTB1, D14, and IPA1 act downstream of OsCCA1 Sugars repress OsCCA1 expression in roots and tiller buds to promote tiller-bud outgrowth
- Additionally, the dual function of OsLHY entirely relied on Hd1, as the double mutant oslhy hd1 showed the same heading date with hd1 under about 11
- Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice
- By MutMap technique, we identified the candidate gene OsLHY from a later flowering mutant lem1 and further confirmed it through genetic complementation, RNA interference knockdown, and CRISPR/Cas9-knockout
- Here we showed that CRISPR/Cas9-engineered mutations in OsLHY caused late flowering in rice only under natural long-day (nLD) and short-day (nSD) conditions, but not artificial SD (10 h light/14 h dark) conditions
- Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the oslhy mutant under LD conditions
- These results suggested that the OsLHY can promote rice flowering mainly through regulating Hd1 and Ehd1
- SDG724 encodes a histone H3K36 methyltransferase, and OsLHY is a vital circadian rhythm transcription factor
- Previously, we reported that SDG724 and OsLHY are two rice heading date regulators in rice
- Connection
- Hd6~CK2, OsLHY, The role of casein kinase II in flowering time regulation has diversified during evolution, This lack of control of the circadian clock by Hd6 CK2alpha might be due to the presence of glutamate in OsLHY (a CCA1 ortholog in rice) instead of the serine at the corresponding CK2 target site in CCA1
- Hd6~CK2, OsLHY, The role of casein kinase II in flowering time regulation has diversified during evolution, We also demonstrated that the other conserved CK2 target sites in OsLHY conferred robust rhythmic expression of OsLHY-LUC under diurnal conditions
- OsLHY, OsPRR1, The role of casein kinase II in flowering time regulation has diversified during evolution, However, this glutamate is critical for the control of the OsPRR1 gene (a rice ortholog of the Arabidopsis TOC1/PRR1 gene) by OsLHY for regulation of the circadian clock
- OsCCT11~OsPRR73, OsLHY, The transcriptional repressor OsPRR73 links circadian clock and photoperiod pathway to control heading date in rice, OsPRR73 bound to the promoters of the floral gene Ehd1 and the circadian gene OsLHY, and significantly suppressed their expression at dawn
- OsCCT11~OsPRR73, OsLHY, The transcriptional repressor OsPRR73 links circadian clock and photoperiod pathway to control heading date in rice, In LL and DD, the oscillatory patterns of the circadian genes OsLHY, OsTOC1, OsGI and OsELF3 were varied in OsPRR73OX and osprr73 mutants
- Ehd1, OsLHY, The transcriptional repressor OsPRR73 links circadian clock and photoperiod pathway to control heading date in rice, OsPRR73 bound to the promoters of the floral gene Ehd1 and the circadian gene OsLHY, and significantly suppressed their expression at dawn
- Hd17~Ef7~OsELF3-1~OsELF3.1~OsELF3, OsLHY, The transcriptional repressor OsPRR73 links circadian clock and photoperiod pathway to control heading date in rice, In LL and DD, the oscillatory patterns of the circadian genes OsLHY, OsTOC1, OsGI and OsELF3 were varied in OsPRR73OX and osprr73 mutants
- OsGI, OsLHY, The transcriptional repressor OsPRR73 links circadian clock and photoperiod pathway to control heading date in rice, In LL and DD, the oscillatory patterns of the circadian genes OsLHY, OsTOC1, OsGI and OsELF3 were varied in OsPRR73OX and osprr73 mutants
- Hd1, OsLHY, Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice, Additionally, the dual function of OsLHY entirely relied on Hd1, as the double mutant oslhy hd1 showed the same heading date with hd1 under about 11
- Hd1, OsLHY, Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice, Together, OsLHY could fine-tune the CDL by directly regulating OsGI, and Hd1 acts as the final effector of CDL downstream of OsLHY
- OsGI, OsLHY, Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice, Subsequent one-hybrid assays in yeast, DNA affinity purification qPCR, and electrophoretic mobility shift assays confirmed OsLHY could directly bind to the CBS element in OsGI promoter
- OsGI, OsLHY, Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice, Moreover, the critical day length (CDL) for function reversal of OsLHY in oslhy (11-12 h) was prolonged in the double mutant oslhy osgi (about 13
- OsGI, OsLHY, Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice, 5 h), indicating that the CDL set by OsLHY was OsGI dependent
- OsGI, OsLHY, Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice, Together, OsLHY could fine-tune the CDL by directly regulating OsGI, and Hd1 acts as the final effector of CDL downstream of OsLHY
- Ehd1, OsLHY, mediated pathways in rice Oryza sativa L.., Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the oslhy mutant under LD conditions
- Ehd1, OsLHY, mediated pathways in rice Oryza sativa L.., Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1
- Ehd1, OsLHY, mediated pathways in rice Oryza sativa L.., These results suggested that the OsLHY can promote rice flowering mainly through regulating Hd1 and Ehd1
- Ghd7, OsLHY, mediated pathways in rice Oryza sativa L.., Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the oslhy mutant under LD conditions
- Ghd7, OsLHY, mediated pathways in rice Oryza sativa L.., Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1
- Hd1, OsLHY, mediated pathways in rice Oryza sativa L.., Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the oslhy mutant under LD conditions
- Hd1, OsLHY, mediated pathways in rice Oryza sativa L.., Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1
- Hd1, OsLHY, mediated pathways in rice Oryza sativa L.., These results suggested that the OsLHY can promote rice flowering mainly through regulating Hd1 and Ehd1
- Hd17~Ef7~OsELF3-1~OsELF3.1~OsELF3, OsLHY, mediated pathways in rice Oryza sativa L.., Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1
- Hd17~Ef7~OsELF3-1~OsELF3.1~OsELF3, OsLHY, mediated pathways in rice Oryza sativa L.., Moreover, the yeast one hybrid assay and electrophoretic mobility shift assay confirmed that OsLHY directly repressed OsGI, RFT1 and OsELF3 by binding to their promoters, which is consistent with that in Arabidopsis
- Hd3a, OsLHY, mediated pathways in rice Oryza sativa L.., Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the oslhy mutant under LD conditions
- Hd3a, OsLHY, mediated pathways in rice Oryza sativa L.., Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1
- OsGI, OsLHY, mediated pathways in rice Oryza sativa L.., Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1
- OsGI, OsLHY, mediated pathways in rice Oryza sativa L.., Moreover, the yeast one hybrid assay and electrophoretic mobility shift assay confirmed that OsLHY directly repressed OsGI, RFT1 and OsELF3 by binding to their promoters, which is consistent with that in Arabidopsis
- OsLHY, RFT1, mediated pathways in rice Oryza sativa L.., Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the oslhy mutant under LD conditions
- OsLHY, RFT1, mediated pathways in rice Oryza sativa L.., Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1
- OsLHY, RFT1, mediated pathways in rice Oryza sativa L.., Moreover, the yeast one hybrid assay and electrophoretic mobility shift assay confirmed that OsLHY directly repressed OsGI, RFT1 and OsELF3 by binding to their promoters, which is consistent with that in Arabidopsis
- OsLHY, SDG724~lvp1, Crosstalk between the Circadian Clock and Histone Methylation., Previously, we reported that SDG724 and OsLHY are two rice heading date regulators in rice
- OsLHY, SDG724~lvp1, Crosstalk between the Circadian Clock and Histone Methylation., SDG724 encodes a histone H3K36 methyltransferase, and OsLHY is a vital circadian rhythm transcription factor
- OsLHY, SDG724~lvp1, Crosstalk between the Circadian Clock and Histone Methylation., To explore the crosstalk between the circadian clock and histone methylation in rice, we studied the relationship between OsLHY and SDG724 via the transcriptome analysis of their single and double mutants, oslhy, sdg724, and oslhysdg724
- OsLHY, SDG724~lvp1, Crosstalk between the Circadian Clock and Histone Methylation., Screening of overlapped DEGs and KEGG pathways between OsLHY and SDG724 revealed that they could control many overlapped pathways indirectly
- OsLHY, SDG724~lvp1, Crosstalk between the Circadian Clock and Histone Methylation., Furthermore, we identified three candidate targets (OsGI, OsCCT38, and OsPRR95) of OsLHY and one candidate target (OsCRY1a) of SDG724 in the clock pathway
- OsLHY, SDG724~lvp1, Crosstalk between the Circadian Clock and Histone Methylation., Our results showed a regulatory relationship between OsLHY and SDG724, which paved the way for revealing the interaction between the circadian clock and histone H3K36 methylation
- OsCCT38~OsPRR59, OsLHY, Crosstalk between the Circadian Clock and Histone Methylation., Furthermore, we identified three candidate targets (OsGI, OsCCT38, and OsPRR95) of OsLHY and one candidate target (OsCRY1a) of SDG724 in the clock pathway
- OsCRY1a, OsLHY, Crosstalk between the Circadian Clock and Histone Methylation., Furthermore, we identified three candidate targets (OsGI, OsCCT38, and OsPRR95) of OsLHY and one candidate target (OsCRY1a) of SDG724 in the clock pathway
- OsGI, OsLHY, Crosstalk between the Circadian Clock and Histone Methylation., Furthermore, we identified three candidate targets (OsGI, OsCCT38, and OsPRR95) of OsLHY and one candidate target (OsCRY1a) of SDG724 in the clock pathway
- OsLHY, OsPRR95, Crosstalk between the Circadian Clock and Histone Methylation., Furthermore, we identified three candidate targets (OsGI, OsCCT38, and OsPRR95) of OsLHY and one candidate target (OsCRY1a) of SDG724 in the clock pathway
- Ghd7, OsLHY, Bifunctional regulators of photoperiodic flowering in short day plant rice., Subsequently, numerous dual function regulators, such as phytochromes, Ghd7, DHT8, OsPRR37, OsGI, OsLHY, and OsELF3, were gradually identified
- Ghd7.1~Hd2~OsPRR37~DTH7, OsLHY, Bifunctional regulators of photoperiodic flowering in short day plant rice., Subsequently, numerous dual function regulators, such as phytochromes, Ghd7, DHT8, OsPRR37, OsGI, OsLHY, and OsELF3, were gradually identified
- Hd17~Ef7~OsELF3-1~OsELF3.1~OsELF3, OsLHY, Bifunctional regulators of photoperiodic flowering in short day plant rice., Subsequently, numerous dual function regulators, such as phytochromes, Ghd7, DHT8, OsPRR37, OsGI, OsLHY, and OsELF3, were gradually identified
- OsGI, OsLHY, Bifunctional regulators of photoperiodic flowering in short day plant rice., Subsequently, numerous dual function regulators, such as phytochromes, Ghd7, DHT8, OsPRR37, OsGI, OsLHY, and OsELF3, were gradually identified
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