D6,OSH15,Oskn3,RLB

2015-01-20 | Categories genes  | Tags vegetative  shoot  meristem  dwarf  panicle  shoot apical meristem  growth  branching  architecture  flower  spikelet  grain  development  seed  lignin  shattering  seed shattering  lignin biosynthesis  spikelet development  map-based cloning  cytokinin  callus 

• Information
  • Symbol: D6,OSH15,Oskn3,RLB
  • MSU: LOC_Os07g03770
  • RAPdb: Os07g0129700
• Publication
  • Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants, 1999, EMBO J.
  • Overexpression of rice OSH genes induces ectopic shoots on leaf sheaths of transgenic rice plants, 2000, Dev Biol.
  • Disruption of KNOX gene suppression in leaf by introducing its cDNA in rice, 2008, Plant Science.
  • SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice, 2013, Planta.
  • Isolation and characterization of a rice homebox gene, OSH15, 1998, Plant Mol Biol.
  • Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, 1999, Plant Mol Biol.
  • Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice, 2002, Plant Mol Biol.
  • KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice, 2001, Genesis.
  • Regional expression of the rice KN1-type homeobox gene family during embryo, shoot, and flower development, 1999, Plant Cell.
  • Positive autoregulation of a KNOX gene is essential for shoot apical meristem maintenance in rice, 2011, Plant Cell.
  • Functional analysis of the conserved domains of a rice KNOX homeodomain protein, OSH15, 2001, Plant Cell.
  • KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes., 2017, Plant Physiol.
  • Characterization of dwarf and narrow leaf dnl-4 mutant in rice, 2020, Plant Signal Behav.
  • The OsmiRNA166b-OsHox32 pair regulates mechanical strength of rice plants by modulating cell wall biosynthesis, 2021, Plant Biotechnol J.
  • RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., 2021, Plant Physiol.
• Genbank accession number
  • AB262805
  • AF050181
  • AK111877
  • AB262805
  • AF050181
  • AK111877
  • AB262805
  • AF050181
  • AK111877
• Key message
  • Whereas Oskn3 transformants showed the most pronounced phenotypic effects during vegetative development, Oskn2 transformants showed relatively mild alterations in the vegetative phase but a more severely affected flower morphology
  • Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired
  • We produced transgenic rice calli, which constitutively express each of four KNOX family class 1 homeobox genes of rice, OSH1, OSH16, OSH15, and OSH71, and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli, which showed continuous growth around their shoot-regenerating stages
  • Whereas the expression of OSH1 continued within the shoot apical meristem, OSH15 expression within the shoot apical meristem ceased but became observable in a ring shaped pattern at the boundaries of some embryonic organs
  • RNA in situ localization data suggest that OSH15 may play roles in the shoot organization during early embryogenesis and thereafter, OSH15 may be involved in morphogenetic events around the shoot apical meristem
  • Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants
  • Ectopic expression of Oskn2 or Oskn3 induced similar defects in panicle branching
  • In transgenic plants carrying the OSH15 promoter-GUS reporter construct, the reporter gene was preferentially expressed in the AZ during young spikelet development
  • The RNA in situ hybridization experiment also showed that OSH15 mRNAs were abundant in the AZ during spikelet development
  • KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes.
  • Knockdown mutations of OSH15 showed reduced seed shattering phenotypes
  • Analyses of osh15 SH5-D double mutants showed that SH5 could not increase the degree of seed shattering when OSH15 was absent, indicating that SH5 functions together with OSH15
  • In addition to the seed shattering phenotype, osh15 mutants displayed dwarfism and accumulated a higher amount of lignin in internodes due to increased expression of the genes involved in lignin biosynthesis
  • We conclude that OSH15 and SH5 form a dimer that enhances seed shattering by directly inhibiting lignin biosynthesis genes
  • Here, we report the map-based cloning of RLB (RICE LATERAL BRANCH) encoding a nuclear-localized, KNOX-type homeobox protein from a rice cytokinin-deficient mutant showing more tillers, sparser panicles, defected floret morphology as well as attenuated shoot regeneration from callus
  • RLB directly binds to the promoter and represses the transcription of OsCKX4, a cytokinin oxidase gene with high abundance in panicle branch meristem
  • We proposed that RLB recruits PRC2 to the OsCKX4 promoter to epigenetically repress its transcription, which suppresses the catabolism of cytokinin, thereby promoting rice lateral branching
• Connection
  • D6~OSH15~Oskn3~RLB, SUI2~ES5, SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice, Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired
  • D6~OSH15~Oskn3~RLB, OsSUI1~OsPSS, SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice, Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, Isolation and characterization of a rice homebox gene, OSH15, We analyzed the in situ mRNA localization of OSH15 through the whole plant life cycle, comparing the expression pattern with that of another rice homeobox gene, OSH1
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, Isolation and characterization of a rice homebox gene, OSH15, Whereas the expression of OSH1 continued within the shoot apical meristem, OSH15 expression within the shoot apical meristem ceased but became observable in a ring shaped pattern at the boundaries of some embryonic organs
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, Two of these, Oskn2 and Oskn3, encode newly described KNOX genes, whereas the third (Oskn1) corresponds to the previously described OSH1 gene
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, Whereas OSH1 was previously proposed to function also in SAM maintenance, Oskn3 may be involved in patterning organ positions, as its expression was found to mark the boundaries of different embryonic organs following SAM formation
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, Transgenic expression of Oskn2 and Oskn3 in tobacco further supported their involvement in cell fate determination, like previously reported for Knotted1 and OSH1 ectopic expression
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, Two of these, Oskn2 and Oskn3, encode newly described KNOX genes, whereas the third (Oskn1) corresponds to the previously described OSH1 gene
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, Transgenic expression of Oskn2 and Oskn3 in tobacco further supported their involvement in cell fate determination, like previously reported for Knotted1 and OSH1 ectopic expression
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, Whereas Oskn3 transformants showed the most pronounced phenotypic effects during vegetative development, Oskn2 transformants showed relatively mild alterations in the vegetative phase but a more severely affected flower morphology
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis, Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice, Here, the transcriptional control of two rice knox genes, Oskn2 and Oskn3, was addressed, showing that the promoter sequences of both genes mediate the initial down-regulation during lateral organ formation, but are insufficient to keep expression in lateral organs stably off
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice, Ectopic expression of Oskn2 or Oskn3 induced similar defects in panicle branching
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice, This was supported by the observation that Oskn3 protein but not Oskn2 could interact with two reported recognition sequences of a KNOX protein from barley
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice, Finally, protein-protein interactions may contribute to the functioning of KNOX proteins, as the ability of Oskn3 and Oskn2 to form heterodimers could be demonstrated
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice, Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice, We produced transgenic rice calli, which constitutively express each of four KNOX family class 1 homeobox genes of rice, OSH1, OSH16, OSH15, and OSH71, and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli, which showed continuous growth around their shoot-regenerating stages
  • D6~OSH15~Oskn3~RLB, OSH71~Oskn2, KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice, We produced transgenic rice calli, which constitutively express each of four KNOX family class 1 homeobox genes of rice, OSH1, OSH16, OSH15, and OSH71, and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli, which showed continuous growth around their shoot-regenerating stages
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, Positive autoregulation of a KNOX gene is essential for shoot apical meristem maintenance in rice, Double mutants of osh1 and d6, a loss-of-function mutant of OSH15, fail to establish the SAM both in embryogenesis and regeneration
  • D6~OSH15~Oskn3~RLB, OSH1~Oskn1, Positive autoregulation of a KNOX gene is essential for shoot apical meristem maintenance in rice, We demonstrate that OSH1 directly binds to five KNOX loci, including OSH1 and OSH15, through evolutionarily conserved cis-elements and that the positive autoregulation of OSH1 is indispensable for its own expression and SAM maintenance
  • D6~OSH15~Oskn3~RLB, qSH1~RIL1, KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes., Co-immunoprecipitation experiments revealed that OSH15 interacts with SH5 and qSH1, two proteins in the BELL homeobox family
  • D6~OSH15~Oskn3~RLB, SH5~RI, KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes., Co-immunoprecipitation experiments revealed that OSH15 interacts with SH5 and qSH1, two proteins in the BELL homeobox family
  • D6~OSH15~Oskn3~RLB, SH5~RI, KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes., Analyses of osh15 SH5-D double mutants showed that SH5 could not increase the degree of seed shattering when OSH15 was absent, indicating that SH5 functions together with OSH15
  • D6~OSH15~Oskn3~RLB, SH5~RI, KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes., Chromatin immunoprecipitation assays demonstrated that both OSH15 and SH5 directly interact with CAD2 chromatin
  • D6~OSH15~Oskn3~RLB, SH5~RI, KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes., We conclude that OSH15 and SH5 form a dimer that enhances seed shattering by directly inhibiting lignin biosynthesis genes
  • D6~OSH15~Oskn3~RLB, OsSRO1a~TWI1, TWI1 regulates cell-to-cell movement of OSH15 to control leaf cell fate., TWI1 regulates cell-to-cell movement of OSH15 to control leaf cell fate.
  • D6~OSH15~Oskn3~RLB, OsSRO1a~TWI1, TWI1 regulates cell-to-cell movement of OSH15 to control leaf cell fate., TWI1 encodes a transcription factor binding protein that interacts with OSH15, a class I KNOTTED1-like homeobox (KNOX) transcription factor
  • D6~OSH15~Oskn3~RLB, OsSRO1a~TWI1, TWI1 regulates cell-to-cell movement of OSH15 to control leaf cell fate., The cell-to-cell trafficking of OSH15 is restricted through its interaction with TWI1
  • D6~OSH15~Oskn3~RLB, OsSRO1a~TWI1, TWI1 regulates cell-to-cell movement of OSH15 to control leaf cell fate., Knockout or knockdown of OSH15 in twi1 rescues the twisted leaf phenotype
  • D6~OSH15~Oskn3~RLB, OFP3, GSK2 Stabilizes OFP3 to Suppress Brassinosteroid Responses in Rice., Furthermore, OFP3 exhibited transcription repressor activity and was able to interact with itself as well as additional BR-related components, including OFP1, OSH1, OSH15, OsBZR1, and GF14c
  • D6~OSH15~Oskn3~RLB, OsOFP6, Rice OVATE family protein 6 regulates leaf angle by modulating secondary cell wall biosynthesis , Protein-protein interaction analysis revealed that OsOFP6 interacts with Oryza sativa homeobox 15 (OSH15), a class I KNOX protein
  • D6~OSH15~Oskn3~RLB, OsOFP6, Rice OVATE family protein 6 regulates leaf angle by modulating secondary cell wall biosynthesis , The interaction of OsOFP6 and OSH15 enhanced the transcriptional activity of OSH15 which binds to the promoter of OsIRX9 (Oryza sativa IRREGULAR XYLEM 9)
  • D6~OSH15~Oskn3~RLB, OSHB4~OsHox32, The OsmiRNA166b-OsHox32 pair regulates mechanical strength of rice plants by modulating cell wall biosynthesis, The suppression of OsCAD2 is synergistic when OsHox32 is co-expressed with OSH15 (Oryza sativa homeobox 15)
  • D6~OSH15~Oskn3~RLB, OSHB4~OsHox32, The OsmiRNA166b-OsHox32 pair regulates mechanical strength of rice plants by modulating cell wall biosynthesis, OsHox32 interacts with OSH15, and the START domain of OsHox32, harbouring the miR166b cleavage site, is required for the interaction of these two proteins
  • D6~OSH15~Oskn3~RLB, GH2~OsCAD2, The OsmiRNA166b-OsHox32 pair regulates mechanical strength of rice plants by modulating cell wall biosynthesis, The suppression of OsCAD2 is synergistic when OsHox32 is co-expressed with OSH15 (Oryza sativa homeobox 15)
  • D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., RLB (RICE LATERAL BRANCH) recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching.
  • D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., RLB directly binds to the promoter and represses the transcription of OsCKX4, a cytokinin oxidase gene with high abundance in panicle branch meristem
  • D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., OsCKX4 over-expression lines phenocopied rlb which showed up-regulated OsCKX4 levels
  • D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., Meanwhile, RLB physically binds to Polycomb repressive complex 2 (PRC2) component OsEMF2b and co-localized with H3K27me3, a suppressing histone modification mediated by PRC2, in the OsCKX4 promoter
  • D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., We proposed that RLB recruits PRC2 to the OsCKX4 promoter to epigenetically repress its transcription, which suppresses the catabolism of cytokinin, thereby promoting rice lateral branching
  • D6~OSH15~Oskn3~RLB, OsEMF2b, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., Meanwhile, RLB physically binds to Polycomb repressive complex 2 (PRC2) component OsEMF2b and co-localized with H3K27me3, a suppressing histone modification mediated by PRC2, in the OsCKX4 promoter
  • D6~OSH15~Oskn3~RLB, DEAP1, DEAP1 encodes a fasciclin-like arabinogalactan protein required for male fertility in rice., Moreover, DEAP1 may interact with two rice D6 PROTEIN KINASE-LIKE3s (OsD6PKL3s), homologs of a known Arabidopsis aperture protein, to affect rice pollen aperture development
  • D6~OSH15~Oskn3~RLB, DLT~OsGRAS-32~D62~GS6~SMOS2, Rice DWARF AND LOW-TILLERING and the homeodomain protein OSH15 interact to regulate internode elongation via orchestrating brassinosteroid signaling and metabolism., DLT physically interacts with OSH15 to co-regulate many genes in seedlings and internodes
  • D6~OSH15~Oskn3~RLB, DLT~OsGRAS-32~D62~GS6~SMOS2, Rice DWARF AND LOW-TILLERING and the homeodomain protein OSH15 interact to regulate internode elongation via orchestrating brassinosteroid signaling and metabolism., OSH15 targets and promotes the expression of the BR receptor gene BR INSENSITIVE1 (OsBRI1), and DLT facilitates this regulation in a dosage-dependent manner
  • D6~OSH15~Oskn3~RLB, DLT~OsGRAS-32~D62~GS6~SMOS2, Rice DWARF AND LOW-TILLERING and the homeodomain protein OSH15 interact to regulate internode elongation via orchestrating brassinosteroid signaling and metabolism., In osh15, dlt, and osh15 dlt, BR levels are higher in seedlings and panicles, but unexpectedly lower in internodes compared with the wild-type
  • D6~OSH15~Oskn3~RLB, DLT~OsGRAS-32~D62~GS6~SMOS2, Rice DWARF AND LOW-TILLERING and the homeodomain protein OSH15 interact to regulate internode elongation via orchestrating brassinosteroid signaling and metabolism., Taken together, our results suggest that DLT interacts with OSH15, which functions in the lower internodes, to modulate rice internode elongation via orchestrating BR signaling and metabolism

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