- Information
- Symbol: RISBZ1,OsbZIP58,OsSMF1
- MSU: LOC_Os07g08420
- RAPdb: Os07g0182000
- Publication
- Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, 2010, Plant Cell Physiol.
- OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm, 2013, J Exp Bot.
- A rice functional transcriptional activator, RISBZ1, responsible for endosperm-specific expression of storage protein genes through GCN4 motif, 2001, J Biol Chem.
- Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, 2009, Plant J.
- Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice, 2008, Plant Physiol.
- Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, 2006, Plant Physiol.
- OsLOL1, a C2C2-type zinc finger protein, interacts with OsbZIP58 to promote seed germination through the modulation of gibberellin biosynthesis in Oryza sativa, 2014, Plant J.
- Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice., 2017, Rice (N Y).
- High temperature inhibits the accumulation of storage materials by inducing alternative splicing of OsbZIP58 during filling stage in rice., 2020, Plant Cell Environ.
- Rice protein-binding microarrays: a tool to detect cis-acting elements near promoter regions in rice, 2021, Planta.
- Genbank accession number
- Key message
- These phenotypes suggest that combinatorial interactions between RISBZ1 and RPBF play an essential role during grain filling
- Compensation and interaction between RISBZ1 and RPBF during grain filling in rice
- Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- ) basic leucine Zipper factor RISBZ1 and rice prolamin box binding factor (RPBF) are transcriptional activators of rice seed storage protein (SSP) genes in vivo
- To ascertain the functions of these trans-activators in seed development, knock-down (KD) transgenic rice plants were generated in which the accumulation of RISBZ1 and RPBF was reduced in an endosperm-specific manner by co-suppression (KD-RISBZ1 and KD-RPBF)
- Storage lipids were accumulated at reduced levels in KD-RISBZ1 and KD-RISBZ1/KD-RPBF seeds
- KD-RPBF and KD-RISBZ1/KD-RPBF seeds exhibited multi-layered aleurone cells
- The functional redundancy and compensation between RISBZ1 and RPBF possibly account for weak effects on the SSP levels in single KD mutants, and help maintain various processes during seed development in rice
- This study showed that OsbZIP58, a bZIP transcription factor, is a key transcriptional regulator controlling starch synthesis in rice endosperm
- OsbZIP58 was expressed mainly in endosperm during active starch synthesis
- OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm
- The RPBF gene is predominantly expressed in maturing endosperm and coordinately expressed with seed storage protein genes, and is involved in the quantitative regulation of genes expressed in the endosperm in cooperation with RISBZ1
- Five different basic leucine zipper cDNA clones, designated RISBZ1-5, have been isolated from a rice seed cDNA library
- RISBZ1 gene expression is restricted to the seed, where it precedes the expression of storage protein genes
- osbzip58 null mutants displayed abnormal seed morphology with altered starch accumulation in the white belly region and decreased amounts of total starch and amylose
- These findings indicate that OsbZIP58 functions as a key regulator of starch synthesis in rice seeds and provide new insights into seed quality control
- Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- Overexpression of both transcription factors gave rise to much higher levels of expression than the sum of individual activities elicited by either RPBF or RISBZ1 alone
- Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression
- When the RISBZ1 promoter was transcriptionally fused to the beta-glucuronidase reporter gene and the chimeric gene was introduced into rice, the beta-glucuronidase gene is specifically expressed in aleurone and subaleurone layer of the developing endosperm
- These findings suggest that the specific expression of transcriptional activator RISBZ1 gene may determine the endosperm specificity of the storage protein genes
- A rice functional transcriptional activator, RISBZ1, responsible for endosperm-specific expression of storage protein genes through GCN4 motif
- Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes
- OsLOL1, a C2C2-type zinc finger protein, interacts with OsbZIP58 to promote seed germination through the modulation of gibberellin biosynthesis in Oryza sativa
- Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice.
- The results of this study suggest that OsSMF1 is one of the key transcription factors that functions in a wide range of seed developmental processes with different specific binding affinities for the three DNA-binding motifs
- OsSMF1 (previously called RISBZ1 or OsbZIP58) is known to interact with GCN4 motifs (TGA(G/C)TCA) to regulate seed storage protein synthesis, and it functions as a key regulator of starch synthesis
- OsSMF1 was shown to bind directly to the promoters of Os03g0168500 (GCN4 motif), patatin-like gene (GCN4 motif), α-globulin (ACGT motif), rice prolamin box-binding factor (RPBF) (ATGA motif), and ONAC024 (GCN4 and ACGT motifs) and to regulate their expression
- Here, we report that Opaque2 like transcription factor OsbZIP58 is a key factor regulating storage material accumulation under HT
- High temperature inhibits the accumulation of storage materials by inducing alternative splicing of OsbZIP58 during filling stage in rice.
- Therefore, OsbZIP58 is a crucial gene in regulating storage material accumulation under HT and lower alternative splicing of OsbZIP58 may contribute to heat tolerance during grain filling
- The OsbZIP58 gene promotes expression of many seed storage protein genes and starch synthesis genes while inhibits expression of some starch hydrolyzing
-amylase genes under HT
- Connection
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Mutation analyses in planta, trans-activation reporter assays in vivo and electrophorestic mobility shift assays in vitro showed that the RPBF-recognizing prolamin box (AAAG) and the RISBZ1-recognizing GCN4 motif (TGAG/CTCA) act as important cis-elements for proper expression of OsLKR/SDH like SSP genes
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Mutation analyses in planta, trans-activation reporter assays in vivo and electrophorestic mobility shift assays in vitro showed that the RPBF-recognizing prolamin box (AAAG) and the RISBZ1-recognizing GCN4 motif (TGAG/CTCA) act as important cis-elements for proper expression of OsLKR/SDH like SSP genes
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- ALK~SSIIa, RISBZ1~OsbZIP58~OsSMF1, OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm, Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression
- OsBEIIb~OsSBEIIb, RISBZ1~OsbZIP58~OsSMF1, OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm, Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression
- BEI~SBE1, RISBZ1~OsbZIP58~OsSMF1, OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm, Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression
- RISBZ1~OsbZIP58~OsSMF1, Wx~qFC6, OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm, Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression
- OsISA2, RISBZ1~OsbZIP58~OsSMF1, OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm, Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression
- ADL1~OsDEK1, RISBZ1~OsbZIP58~OsSMF1, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- OsSAL1~RHL, RISBZ1~OsbZIP58~OsSMF1, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, ) basic leucine Zipper factor RISBZ1 and rice prolamin box binding factor (RPBF) are transcriptional activators of rice seed storage protein (SSP) genes in vivo
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, To ascertain the functions of these trans-activators in seed development, knock-down (KD) transgenic rice plants were generated in which the accumulation of RISBZ1 and RPBF was reduced in an endosperm-specific manner by co-suppression (KD-RISBZ1 and KD-RPBF)
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, The accumulation of most SSPs changed little between individual KD mutants and wild-type plants, whereas a double KD mutant (KD-RISBZ1/KD-RPBF) resulted in a significant reduction of most SSP gene expression and accumulation
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Storage lipids were accumulated at reduced levels in KD-RISBZ1 and KD-RISBZ1/KD-RPBF seeds
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, KD-RPBF and KD-RISBZ1/KD-RPBF seeds exhibited multi-layered aleurone cells
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, These phenotypes suggest that combinatorial interactions between RISBZ1 and RPBF play an essential role during grain filling
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, The functional redundancy and compensation between RISBZ1 and RPBF possibly account for weak effects on the SSP levels in single KD mutants, and help maintain various processes during seed development in rice
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Physical interaction between RISBZ1 and RPBF may ensure that these processes are carried out properly
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice
- OsCR4~MIS2, RISBZ1~OsbZIP58~OsSMF1, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Synergism between RPBF and RISBZ1 recognizing the GCN4 motif [TGA(G/C)TCA] was observed in the expression of many storage protein genes
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Overexpression of both transcription factors gave rise to much higher levels of expression than the sum of individual activities elicited by either RPBF or RISBZ1 alone
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Furthermore, mutation of recognition sites suppressed reciprocal trans-activation ability, indicating that there are mutual interactions between RISBZ1 and RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, The RPBF gene is predominantly expressed in maturing endosperm and coordinately expressed with seed storage protein genes, and is involved in the quantitative regulation of genes expressed in the endosperm in cooperation with RISBZ1
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes
- OsLOL1, RISBZ1~OsbZIP58~OsSMF1, OsLOL1, a C2C2-type zinc finger protein, interacts with OsbZIP58 to promote seed germination through the modulation of gibberellin biosynthesis in Oryza sativa, OsLOL1, a C2C2-type zinc finger protein, interacts with OsbZIP58 to promote seed germination through the modulation of gibberellin biosynthesis in Oryza sativa
- OsPht1;8~OsPT8, RISBZ1~OsbZIP58~OsSMF1, Phosphate transporter OsPht1;8 in rice plays an important role in phosphorus redistribution from source to sink organs and allocation between embryo and endosperm of seeds., In this study, we knocked down expression of a phosphate transporter gene OsPht1;8 (OsPT8) selectively in shoot and/or in seed endosperm by RNA-interference using RISBZ1 and GluB-1 promoter (designate these transgenic lines as SSRi and EnSRi), respectively, to characterize the role of OsPT8 in P redistribution of rice
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice., OsSMF1 was shown to bind directly to the promoters of Os03g0168500 (GCN4 motif), patatin-like gene (GCN4 motif), α-globulin (ACGT motif), rice prolamin box-binding factor (RPBF) (ATGA motif), and ONAC024 (GCN4 and ACGT motifs) and to regulate their expression
- OsWOX13, RISBZ1~OsbZIP58~OsSMF1, Rice protein-binding microarrays: a tool to detect cis-acting elements near promoter regions in rice, To confirm the efficiency of RPBM technology, we selected two previously studied TFs, OsWOX13 and OsSMF1, and an uncharacterized TF, OsWRKY34
- OsWOX13, RISBZ1~OsbZIP58~OsSMF1, Rice protein-binding microarrays: a tool to detect cis-acting elements near promoter regions in rice, We identified the ATTGATTG and CCACGTCA DNA-binding sequences of OsWOX13 and OsSMF1, respectively
- OsWOX13, RISBZ1~OsbZIP58~OsSMF1, Rice protein-binding microarrays: a tool to detect cis-acting elements near promoter regions in rice, In total, 635 and 932 putative feature genes were identified for OsWOX13 and OsSMF1, respectively
- OsDML4, RISBZ1~OsbZIP58~OsSMF1, A new demethylase gene, OsDML4, is involved in high temperature-increased grain chalkiness in rice., The expression of two transcription factor genes RISBZ1 and RPBF was significantly decreased in the osdml4 mutants, which caused adverse effects on the formation of protein bodies (PBs) with greatly decreased PB-II number, and incomplete and abnormally shaped PB-IIs
- OsbZIP14, RISBZ1~OsbZIP58~OsSMF1, Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal OsbZIP14 Function in Rice in Response to Heat Stress., Under high-temperature treatment, it was also demonstrated that in the OsbZIP14 mutant, the expression of the OsbZIP58 gene, a key regulator of rice seed storage protein (SSP) accumulation, was upregulated
- OsbZIP14, RISBZ1~OsbZIP58~OsSMF1, Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal OsbZIP14 Function in Rice in Response to Heat Stress., Furthermore, bimolecular fluorescence complementation (BiFC) experiments uncovered a direct interaction between OsbZIP14 and OsbZIP58
- OsbZIP14, RISBZ1~OsbZIP58~OsSMF1, Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal OsbZIP14 Function in Rice in Response to Heat Stress., Our results suggested that OsbZIP14 acts as a key TF gene through the concerted action of OsbZIP58 and OsbZIP14 during rice filling under heat stress
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