- Information
- Symbol: OsNRAMP5
- MSU: LOC_Os07g15370
- RAPdb: Os07g0257200
- Publication
- Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport, 2012, Sci Rep.
- Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice, 2012, Proc Natl Acad Sci U S A.
- Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield., 2017, Sci Rep.
- Mutation at Different Sites of Metal Transporter Gene OsNramp5 Affects Cd Accumulation and Related Agronomic Traits in Rice Oryza sativa L.., 2019, Front Plant Sci.
- Altered root structure affects both expression and cellular localization of transporters for mineral element uptake in rice., 2019, Plant Cell Physiol.
- Effects of external Mn2+ activities on OsNRAMP5 expression level and Cd accumulation in indica rice., 2020, Environ Pollut.
- Overexpression of the manganese/cadmium transporter OsNRAMP5 reduces cadmium accumulation in rice grain, 2020, J Exp Bot.
- Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice, 2020, Int J Mol Sci.
- Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, 2020, PLoS One.
- Univariate and Multivariate QTL Analyses Reveal Covariance Among Mineral Elements in the Rice Ionome, 2021, Front Genet.
- Knockout of OsNRAMP5 enhances rice tolerance to cadmium toxicity in response to varying external cadmium concentrations via distinct mechanisms., 2022, Sci Total Environ.
- A weak allele of OsNRAMP5 confers moderate cadmium uptake while avoiding manganese deficiency in rice., 2022, J Exp Bot.
- OsNRAMP5 Is a Major Transporter for Lead Uptake in Rice., 2022, Environ Sci Technol.
- Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain., 2022, Nat Food.
- Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice., 2022, J Hazard Mater.
- Genbank accession number
- Key message
- OsNRAMP5 RNAi (OsNRAMP5i) plants accumulated less Mn in the roots, and less Mn and Fe in shoots, and xylem sap
- The suppression of OsNRAMP5 promoted Cd translocation to shoots, highlighting the importance of this gene for Cd phytoremediation
- Functional analysis revealed that the defective transporter protein encoded by the mutant osnramp5 greatly decreases Cd uptake by roots, resulting in decreased Cd in the straw and grain
- OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem
- OsNRAMP5 localized to the plasma membrane, and complemented the growth of yeast strains defective in Mn, Fe, and Cd transport
- These data reveal that OsNRAMP5 contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development
- Hydroponic culture showed that Cd concentrations in shoots and roots of osnramp5 mutants were dramatically decreased, resulting in rescue of impaired growth in high Cd condition
- Here, we report the development of new indica rice lines with low Cd accumulation and no transgenes by knocking out the metal transporter gene OsNramp5 using CRISPR/Cas9 system
- In particular, the plant yield was not significantly affected in osnramp5 mutants
- OsNramp5 is a key gene involved in the control of the uptake of Cd, Mn, and other metal ions by rice root cells
- The determination of metal content and the statistics of related agronomic traits revealed that the functionally deficient OsNramp5 not only significantly reduced the accumulation of Cd in the grains of the mutants but also affected rice yield and quality
- Additionally, we also found that the increase in the concentration of Mn in the soil restored the phenotype of the declined yield and quality due to the functional deficiency of OsNramp5
- Mutation at Different Sites of Metal Transporter Gene OsNramp5 Affects Cd Accumulation and Related Agronomic Traits in Rice (Oryza sativa L.).
- In the present study, three Huanghuazhan-based OsNramp5 mutants [LCH1 (Low Cadmium Huanghuazhan 1), LCH2 (Low Cadmium Huanghuazhan 2), and LCH3 (Low Cadmium Huanghuazhan 3)] were obtained using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology
- However, with the decrease of OsNramp5 mutation degree, its effects on chlorenchyma Mn accumulation, yield, and quality were also diminished
- The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNramp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type rice
- Besides, OsNRAMP5 expression level in roots seemed more related to root Mn accumulation
- At last, multi-level regulating and processing of the process from gene expression to protein translation might account for the inconsistent relationship between root OsNRAMP5 expression level and Cd accumulation in roots
- Manganese (Mn) transporter OsNRAMP5 was widely reported to regulate cadmium (Cd) uptake in rice
- The fact that function of OsNRAMP5 mainly focuses on Mn uptake, together with the fact that many transporter genes involved in Cd uptake might result in the insignificant correlation between OsNRAMP5 expression level and Cd accumulation in roots
- Overexpression of OsNRAMP5 decreased Cd translocation from roots to shoots by disrupting radial transport of Cd into the stele for xylem loading, demonstrating the effect of transporter localization and polarity on ion homeostasis
- Overexpressed OsNRAMP5 protein was localized to the plasma membrane of all cell types in the root tips and lateral root primordia without polarity
- Overexpression of the manganese/cadmium transporter OsNRAMP5 reduces cadmium accumulation in rice grain
- When grown in three Cd-contaminated paddy soils, OsNRAMP5 overexpression decreased grain Cd concentration by 49-94% compared with wild type
- The effect of OsNRAMP5 knockout on reducing root Cd uptake weakened, however its effect on improving root-to-shoot Cd translocation was constant with increasing environmental Cd concentrations
- Knockout of OsNRAMP5 enhances rice tolerance to cadmium toxicity in response to varying external cadmium concentrations via distinct mechanisms.
- It is vital to evaluate the effects of knocking out OsNRAMP5 on Cd and Mn accumulation, as well as Cd tolerance of rice plants in response to varying environmental Cd concentrations, and to uncover the underlying mechanism, which until now, has remained largely unexplored
- OsNRAMP5 is a transporter responsible for cadmium (Cd) and manganese (Mn) uptake and root-to-shoot translocation of Mn in rice plants
- Among several genes involved in rice Cd accumulation, the loss of function of OsNRAMP5 is known to be effective in reducing grain concentration by inhibiting root uptake
- With the aim of improving Mn uptake in OsNRAMP5 mutants while still restricting the grain Cd concentration below the upper limit of international standards, we identified a novel OsNRAMP5 allele encoding a protein in which glutamine (Q) at position 337 was replaced by lysine (K)
- However, disruption of this gene simultaneously decreases manganese (Mn) uptake because OsNRAMP5 is a major Mn transporter
- A weak allele of OsNRAMP5 confers moderate cadmium uptake while avoiding manganese deficiency in rice.
- Knockout of OsNRAMP5 in rice by CRISPR/Cas9 gene editing resulted in significant decreases in root uptake of Pb and accumulation in rice shoots
- When grown in Pb-contaminated paddy soil, OsNRAMP5 knockout mutants accumulated approximately 50 and 70% lower Pb concentrations in the grain and straw, respectively, than the wild type
- OsNRAMP5 Is a Major Transporter for Lead Uptake in Rice.
- These results indicate that OsNRAMP5 is a major transporter for Pb uptake in rice, in addition to its role in the uptake of manganese and cadmium
- Higher expression of OsNramp5 increased uptake of Cd and Mn into the root cells but decreased Cd release to the xylem
- Connection
- OsHMA2~OsHMA2v, OsNRAMP5, Silicon reduces cadmium accumulation by suppressing expression of transporter genes involved in cadmium uptake and translocation in rice., The expression level of OsNramp5 and OsHMA2 was down-regulated by Si in the WTs, but not in the mutants
- Lsi1~OsNIP2;1~OsLsi1, OsNRAMP5, Altered root structure affects both expression and cellular localization of transporters for mineral element uptake in rice., The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNramp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type rice
- Lsi2~OsLsi2, OsNRAMP5, Altered root structure affects both expression and cellular localization of transporters for mineral element uptake in rice., The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNramp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type rice
- OsNRAMP1, OsNRAMP5, OsNRAMP1 contributes to cadmium and manganese uptake in rice , The knockout of OsNRAMP1 had smaller effects on Cd and Mn uptake than knockout of OsNRAMP5, while knockout of both genes resulted in large decreases in the uptake of the two metals
- OsNRAMP1, OsNRAMP5, OsNRAMP1 contributes to cadmium and manganese uptake in rice , Taken together, OsNRAMP1 contributes significantly to the uptake of Mn and Cd in rice and the functions of OsNRAMP1 and OsNRAMP5 are similar but not redundant
- OsNRAMP1, OsNRAMP5, Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice, Our results showed that the supplement of 5 mM Ca significantly reduced Cd uptake by rice roots, because of their competition for Ca-permeable channels as an absorption site and Ca-induced downregulation of OsNRAMP1 and OsNRAMP5
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Similarly, expression level of CAL1, OsNRAMP5, and OsNRAMP1 significantly increased in roots in high Cd and Mn deficient treatments
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsIRT1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsIRT1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsIRT1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsNAAT1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsNAAT1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsNAAT1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsNRAMP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsNRAMP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsNRAMP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Similarly, expression level of CAL1, OsNRAMP5, and OsNRAMP1 significantly increased in roots in high Cd and Mn deficient treatments
- OsNRAMP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsNRAMP5, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsNRAMP5, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsNRAMP5, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsHMA4, OsNRAMP5, Univariate and Multivariate QTL Analyses Reveal Covariance Among Mineral Elements in the Rice Ionome, The candidate genes underlying the QTL clusters with elemental QTLs, PC-QTLs and aPC-QTLs co-localized were identified, including OsHMA4 and OsNRAMP5
- OsNRAMP5, OsYSL2~CF1, CF1 reduces grain-cadmium levels in rice Oryza sativa., Further analysis showed that high expression levels of CF1 improve Fe nutrition in the shoots, subsequently inhibiting Cd uptake by systemically inhibiting expression of the main Cd uptake gene OsNramp5 in the roots
- OsLCD, OsNRAMP5, Generation of low-cadmium rice germplasms via knockout of OsLCD using CRISPR/Cas9., We also generated osnramp5 single mutants and oslcd osnramp5 double mutants in the indica background
- OsLCD, OsNRAMP5, Generation of low-cadmium rice germplasms via knockout of OsLCD using CRISPR/Cas9., In the presence of Cd, Cd concentrations in the brown rice and shoots of oslcd osnramp5 double mutants were significantly decreased compared with WTs as well as osnramp single mutants
- OsLCD, OsNRAMP5, Generation of low-cadmium rice germplasms via knockout of OsLCD using CRISPR/Cas9., Our results suggested that OsLCD knockout may reduce Cd accumulation alone or in combination with other knockout mutations in a variety of rice genotypes; unlike OsNramp5 mutations, OsLCD knockout did not reduce essential element contents
- OsNRAMP1, OsNRAMP5, Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice., Furthermore, MT applied exogenously down-regulated the transcription level of Natural Resistance-Associated Macrophage Protein 1 (OsNRAMP1), OsNRAMP5, a major facilitator superfamily gene (OsCd1), and IRON-REGULATED TRANSPORTER 1 (OsIRT1), all of which were responsible for Cd intake, thus less Cd was entered into roots
- OsCD1~OsCSLD4~NRL1~ND1~sle1~DNL1, OsNRAMP5, Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice., Furthermore, MT applied exogenously down-regulated the transcription level of Natural Resistance-Associated Macrophage Protein 1 (OsNRAMP1), OsNRAMP5, a major facilitator superfamily gene (OsCd1), and IRON-REGULATED TRANSPORTER 1 (OsIRT1), all of which were responsible for Cd intake, thus less Cd was entered into roots
- OsIRT1, OsNRAMP5, Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice., Furthermore, MT applied exogenously down-regulated the transcription level of Natural Resistance-Associated Macrophage Protein 1 (OsNRAMP1), OsNRAMP5, a major facilitator superfamily gene (OsCd1), and IRON-REGULATED TRANSPORTER 1 (OsIRT1), all of which were responsible for Cd intake, thus less Cd was entered into roots
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