eIF4G

• Symbol: eIF4G
• MSU: LOC_Os07g36940
• RAPdb: Os07g0555200

• Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus., 2018, Plant Biotechnol J.
• Single nucleotide polymorphisms in a gene for translation initiation factor eIF4G of rice Oryza sativa associated with resistance to Rice tungro spherical virus., 2010, Mol Plant Microbe Interact.
• Genome Editing of Rice eIF4G Loci Confers Partial Resistance to Rice Black-Streaked Dwarf Virus, 2021, Viruses.

Genbank accession number

• Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus.
• To develop new sources of resistance to RTD, mutations in eIF4G were generated using the CRISPR/Cas9 system in the RTSV-susceptible variety IR64, widely grown across tropical Asia
• Among various mutated eIF4G alleles examined, only those resulting in in-frame mutations in SVLFPNLAGKS residues (mainly NL), adjacent to the YVV residues, conferred resistance
• Genome Editing of Rice eIF4G Loci Confers Partial Resistance to Rice Black-Streaked Dwarf Virus
• To develop novel resources for resistance to RBSDV through CRISPR/Cas9-mediated genome editing, a guide RNA sequence targeting exon 1 of eIF4G was designed and cloned into a binary vector, pHUE401
• These results indicate that site-specific mutation of rice eIF4G successfully conferred partial resistance specific to RBSDV associated with less transcription of eIF4G in mutants
• However, the eif4g mutant plants showed the same sensitivity to rice stripe virus (RSV) infection as wild-type plants