ABC1,OsFd-GOGAT,SPL32,ES7

• Symbol: ABC1,OsFd-GOGAT,SPL32,ES7
• MSU: LOC_Os07g46460
• RAPdb: Os07g0658400

• Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies, 2016, Molecular plant.
• The ferredoxin-dependent glutamate synthase OsFd-GOGAT participates in leaf senescence and the nitrogen remobilization in rice., 2016, Mol Genet Genomics.
• Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice., 2017, Sci Rep.
• ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice, 2017, Plant Science.
• Nitrogen Mediates Flowering Time and Nitrogen Use Efficiency via Floral Regulators in Rice, 2020, Curr Biol.

Genbank accession number

• Metabolomics analysis revealed the accumulation of excessive amount of amino acids with high N/C ratio (Gln and Asn) and several intermediates in the tricarboxylic acid cycle in abc1-1, suggesting that ABC1 plays a critical role in regulating nitrogen assimilation and carbon-nitrogen balance
• ), the contribution of OsFd-GOGAT to rice foliar nitrogen metabolism remains little up-to-date
• The results in this study suggested that OsFd-GOGAT might participate in nitrogen remobilization during leaf senescence, which provides a potential way to improve nitrogen use efficiency in rice
• The spl32 plants displayed early leaf senescence, identified by disintegration of chloroplasts as cellular evidence, dramatically decreased contents of chlorophyll, up-regulation of superoxide dismutase enzyme activity and malondialdehyde, as physiological characteristic, and both up-regulation of senescence-induced STAY GREEN gene and senescence-associated transcription factors, and down-regulation of photosynthesis-associated genes, as molecular indicators
• qRT-PCR analysis indicated that several genes related to nitrogen metabolism were differentially expressed in es7
• In addition, when seedlings are grown under increasing nitrogen concentrations (NH4NO3) for 15 days, the contents of chlorophyll a, chlorophyll b and total chlorophyll were significantly lower in es7
• Our results demonstrated that ES7 is involved in nitrogen metabolism, effects chlorophyll synthesis, and may also associated with photorespiration, impacting leaf senescence in rice
• ES7 was expressed constitutively, and the ES7 protein was localized in chloroplast
• When es7 plants are grown under photorespiration-suppressed conditions (high CO2), the senescence phenotype and chlorophyll content are rescued
• The leaves of the es7 mutant begin to senesce at the tillering stage about 60 day after sowing, and become increasingly senescent as the plants develop at the heading stage

• ABC1~OsFd-GOGAT~SPL32~ES7, ARE2, Regulation of nitrogen starvation responses by the alarmone pppGpp in rice., Here, we show that ARE2 (for ABC1 REPRESSOR2) is a key regulator of nitrogen starvation responses in rice
• ABC1~OsFd-GOGAT~SPL32~ES7, ARE2, Regulation of nitrogen starvation responses by the alarmone pppGpp in rice., The are2 mutations partially rescue the nitrogen-deficient phenotype of abc1 and the are2 mutants show enhanced tolerance to nitrogen deficiency, suggesting that ARE2 genetically interacts with ABC1/Fd-GOGAT