OsCW-ZF7,WG7

• Symbol: OsCW-ZF7,WG7
• MSU: LOC_Os07g47360
• RAPdb: Os07g0669800,Os07g0669900

• Functional characterization of rice CW-domain containing zinc finger proteins involved in histone recognition, 2017, Plant Science.
• Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., 2019, Plant J.
• Bin-based genome-wide association studies reveal superior alleles for improvement of appearance quality using a 4-way MAGIC population in rice, 2020, J Adv Res.

Genbank accession number

• Knockout of OsCW-ZF7 caused defective development of awns
• Here, we identified three cysteine-tryptophan (CW)-domain containing zinc finger (ZF) proteins involved in histone recognition, namely OsCW-ZF3, OsCW-ZF5 and OsCW-ZF7
• The grain size of WG7 knockout mutants and WG7 overexpression lines indicated that WG7 is a positive regulator of grain size
• EMSAs and ChIP-qPCR assay confirmed that WG7 directly bound to the promoter of OsMADS1, a grain size gene, and thereby significantly activated its expression
• These findings demonstrated that WG7 upregulated OsMADS1 expression by directly binding to its promoter, enhanced histone H3K4me3<U+00A0>enrichment in the promoter and ultimately increased grain width
• WG7 underwent directional selection due to the poor fertility of the nonfunctional mutant
• WG7 encodes a cysteine-tryptophan (CW) domain-containing transcriptional activator

• OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., EMSAs and ChIP-qPCR assay confirmed that WG7 directly bound to the promoter of OsMADS1, a grain size gene, and thereby significantly activated its expression
• OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., Compared with the wild-type, deletion mutants of the cis-element motif exhibited lower expression of OsMADS1 and produced narrower grains, implicating the requirement of this motif for WG7 function
• OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., ChIP-qPCR assays showed that WG7 enhanced histone H3K4me3 enrichment in the promoter of OsMADS1
• OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., These findings demonstrated that WG7 upregulated OsMADS1 expression by directly binding to its promoter, enhanced histone H3K4me3<U+00A0>enrichment in the promoter and ultimately increased grain width
• OsCW-ZF5, OsCW-ZF7~WG7, Functional characterization of rice CW-domain containing zinc finger proteins involved in histone recognition., Here, we identified three cysteine-tryptophan (CW)-domain containing zinc finger (ZF) proteins involved in histone recognition, namely OsCW-ZF3, OsCW-ZF5 and OsCW-ZF7
• OsCW-ZF5, OsCW-ZF7~WG7, Functional characterization of rice CW-domain containing zinc finger proteins involved in histone recognition., In contrast to OsCW-ZF3 and OsCW-ZF5 where the CW domains bind histone H3 lysine 4 with different methylated forms (H3K4me), the CW domain from OsCW-ZF7 recognizes only trimethylated histone H3 lysine 4 (H3K4me3)