- Information
- Symbol: OsFAD8
- MSU: LOC_Os07g49310
- RAPdb: Os07g0693800
- Publication
- Characterization of a rice Oryza sativa L. gene encoding a temperature-dependent chloroplast omega-3 fatty acid desaturase, 2006, Biochem Biophys Res Commun.
- Effects of low temperature stress on rice Oryza sativa L. plastid omega-3 desaturase gene, OsFAD8 and its functional analysis using T-DNA mutants, 2009, Plant Cell, Tissue and Organ Culture (PCTOC).
- Identification and evaluation of omega-3 fatty acid desaturase genes for hyperfortifying alpha-linolenic acid in transgenic rice seed, 2012, J Exp Bot.
- Rice mutants deficient in omega-3 fatty acid desaturase FAD8 fail to acclimate to cold temperatures, 2016, Plant Physiology and Biochemistry.
- Genbank accession number
- Key message
- RNA in situ hybridization showed that the accumulation of Osfad8 mRNA was abundant in leaves but hardly detectable in roots
- Fatty acid analysis of homozygous OsFAD8 T-DNA knockout mutant and wild type plants have exhibited changes in fatty acid composition after cold stress, thus confirming that OsFAD8 gene codes for omega-3 fatty acid desaturase activity at low temperature
- Photosynthetic efficiency and recovery of OsFAD8 knockout mutants are significantly reduced after cold stress as compared to those of wild type plants
- The Osfad8 transcript level in leaves was much higher at 15 degrees C than at normal temperature (25 degrees C)
- These data suggest that Osfad8 encodes a temperature-dependent chloroplast omega-3 fatty acid desaturase
- Expression of two rice plastidial omega-3 desaturase genes, OsFAD7 and OsFAD8 under different temperatures has been investigated
- Transcript levels of OsFAD7 increased at high temperature; while those of OsFAD8 increased at low temperatures
- Characterization of OsFAD8 suggests that it has a functional role in maintaining levels of trienoic fatty acids and stress tolerance at low temperatures
- Effects of low temperature stress on rice (Oryza sativa L.) plastid omega-3 desaturase gene, OsFAD8 and its functional analysis using T-DNA mutants
- A cDNA, designated Osfad8, encoding a chloroplast omega-3 fatty acid desaturase responsible for trienoic fatty acid formation, was isolated from the leaves of Oryza sativa L
- At room temperature (28 C), osfad8 plants exhibited significant alterations in fatty acid (FA) unsaturation for all four investigated plastidic lipid classes
- Taken together, our findings suggest that FA unsaturation by OsFAD8 is crucial for the acclimation of higher plants to low-temperature stress
- Connection
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