OsPLC1

• Symbol: OsPLC1
• MSU: LOC_Os07g49330
• RAPdb: Os07g0694000

• A phosphoinositide-specific phospholipase C pathway elicits stress-induced Ca2+ signals and confers salt tolerance to rice., 2017, New Phytol.
• The phosphoinositide-specific phospholipase C1 modulates flowering time and grain size in rice., 2022, Planta.

Genbank accession number

• Salt stress induced the recruitment of OsPLC1 from cytoplasm to plasma membrane, where it hydrolyzed PtdIns4P
• In addition, the enlargement of grain size was observed in OE plants, however, the reduction of grain size was noticed in osplc1 mutants
• The increase in the grain size and the grain yield of OE lines were associated with the improvement of cell length and expression levels of a set of genes related to cell expansion, contrarily, the decrease in osplc1 mutant grain size and yield were linked to declined cell length and expression levels of related genes
• No significant differences, in terms of the grain quality of mature seeds, were found in OE and osplc1 mutants, with compared to those in Nipponbare (Nip)
• In summary, our study suggests that OsPLC1 could modulate rice flowering time and grain size
• Preferential expression of OsPLC1 is detected at the heading stage of rice, OsPLC1 overexpression results in early flowering, increased-grain size and yield; however, opposing phenotypes produced in the osplc1 mutants
• The expression levels of subset genes, which are involved in the control of flowering time in rice, were altered in the plants of OE and osplc1
• OsPLC1 overexpression (OE) produced rice plants with early flowering, whereas OsPLC1 loss-of-function led to delay in flowering

• GF14b~OsGF14b, OsPLC1, A 14-3-3 protein positively regulates rice salt tolerance by stabilizing phospholipase C1., Here, we identified a 14-3-3 protein, OsGF14b, as an interaction partner of OsPLC1
• GF14b~OsGF14b, OsPLC1, A 14-3-3 protein positively regulates rice salt tolerance by stabilizing phospholipase C1., Similar to OsPLC1, OsGF14b also positively regulates rice salt tolerance, and their interaction can be promoted by NaCl stress
• GF14b~OsGF14b, OsPLC1, A 14-3-3 protein positively regulates rice salt tolerance by stabilizing phospholipase C1., OsGF14b also positively regulated the hydrolysis activity of OsPLC1, and is essential to NaCl-induced activation of rice PI-PLCs
• GF14b~OsGF14b, OsPLC1, A 14-3-3 protein positively regulates rice salt tolerance by stabilizing phospholipase C1., We further discovered that OsPLC1 was degraded via ubiquitin-proteasome pathway, and OsGF14b could inhibit the ubiquitination of OsPLC1 to protect OsPLC1 from degradation
• GF14b~OsGF14b, OsPLC1, A 14-3-3 protein positively regulates rice salt tolerance by stabilizing phospholipase C1., Under salt stress, the OsPLC1 protein level in osgf14b was lower than the corresponding value of WT, whereas overexpression of OsGF14b results in a significant increase of OsPLC1 stability
• GF14b~OsGF14b, OsPLC1, A 14-3-3 protein positively regulates rice salt tolerance by stabilizing phospholipase C1., Taken together, we propose that OsGF14b can interact with OsPLC1 and promote its activity and stability, thereby improving rice salt tolerance