TOP6A2,OsTOP6A2,OsSPO11-2

2015-01-20 | Categories genes  | Tags cytokinin  auxin  flower  pollen  panicle  meiosis  sterile 

• Information
  • Symbol: TOP6A2,OsTOP6A2,OsSPO11-2
  • MSU: LOC_Os08g06050
  • RAPdb: Os08g0156900
• Publication
  • The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay, 2012, BMC Mol Biol.
  • OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB, 2011, PLoS One.
  • Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, 2006, FEBS J.
  • Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, 2020, J Exp Bot.
• Genbank accession number
  • AJ605583
• Key message
  • The expression of OsTOP6A2, OsTOP6A3 and OsTOP6B was differentially regulated by the plant hormones, auxin, cytokinin, and abscisic acid
  • The transcript abundance of OsTOP6A2, OsTOP6A3 and OsTOP6B was also higher in prepollinated flowers and callus
  • Similar to Osspo11-1, the pollen mother cells of Osspo11-2 T1 progeny plants, showed an absence of bivalent formation at metaphase I, aberrant segregation of homologous chromosomes and formation of nonviable tetrads
  • Similar to Osspo11-1, biallelic mutations in OsSPO11-2 first exon led to complete panicle sterility, and consequently, none of these primary transformants produced seeds
  • Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis
  • Altogether, this study demonstrates that whereas OsSPO11-1 and OsSPO11-2 are the likely orthologs of Arabidopsis AtSPO11-1 and AtSPO11-2, the additional OsSPO11-4 gene has no major role in wild-type rice meiosis
  • As expected, we observed very few DMC1 foci (19 on average) in the sterile Osspso11-1 mutant but surprisingly 85 foci in the sterile Osspo11-2 mutant, indicative of a divergent function between these two SPO11 genes in rice
• Connection
  • OsTOP6B, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, The transcript abundance of OsTOP6A2, OsTOP6A3 and OsTOP6B was also higher in prepollinated flowers and callus
  • OsTOP6B, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, The expression of OsTOP6A2, OsTOP6A3 and OsTOP6B was differentially regulated by the plant hormones, auxin, cytokinin, and abscisic acid
  • OsTOP6B, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, Yeast two-hybrid analysis revealed that the full-length OsTOP6B protein interacts with both OsTOP6A2 and OsTOP6A3, but not with OsTOP6A1
  • OsTOP6A3, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, The transcript abundance of OsTOP6A2, OsTOP6A3 and OsTOP6B was also higher in prepollinated flowers and callus
  • OsTOP6A3, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, The expression of OsTOP6A2, OsTOP6A3 and OsTOP6B was differentially regulated by the plant hormones, auxin, cytokinin, and abscisic acid
  • OsTOP6A3, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, Yeast two-hybrid analysis revealed that the full-length OsTOP6B protein interacts with both OsTOP6A2 and OsTOP6A3, but not with OsTOP6A1
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants, Yeast two-hybrid analysis revealed that the full-length OsTOP6B protein interacts with both OsTOP6A2 and OsTOP6A3, but not with OsTOP6A1
  • DMC1A~DMC1~OsDMC1A, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, As expected, we observed very few DMC1 foci (19 on average) in the sterile Osspso11-1 mutant but surprisingly 85 foci in the sterile Osspo11-2 mutant, indicative of a divergent function between these two SPO11 genes in rice
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, To clarify the roles of these SPO11 paralogs, we used CRISPR/Cas9 mutagenesis to induce null mutants in OsSPO11-1, OsSPO11-2 and OsSPO11-4
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Similar to Osspo11-1, biallelic mutations in OsSPO11-2 first exon led to complete panicle sterility, and consequently, none of these primary transformants produced seeds
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Similar to Osspo11-1, the pollen mother cells of Osspo11-2 T1 progeny plants, showed an absence of bivalent formation at metaphase I, aberrant segregation of homologous chromosomes and formation of nonviable tetrads
  • OsSPO11-1~OsTOP6A1, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Altogether, this study demonstrates that whereas OsSPO11-1 and OsSPO11-2 are the likely orthologs of Arabidopsis AtSPO11-1 and AtSPO11-2, the additional OsSPO11-4 gene has no major role in wild-type rice meiosis
  • OsSpo11-4~OsSPO11D, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis
  • OsSpo11-4~OsSPO11D, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, To clarify the roles of these SPO11 paralogs, we used CRISPR/Cas9 mutagenesis to induce null mutants in OsSPO11-1, OsSPO11-2 and OsSPO11-4
  • OsSpo11-4~OsSPO11D, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Altogether, this study demonstrates that whereas OsSPO11-1 and OsSPO11-2 are the likely orthologs of Arabidopsis AtSPO11-1 and AtSPO11-2, the additional OsSPO11-4 gene has no major role in wild-type rice meiosis

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