OsCCA1,Nhd1

• Symbol: OsCCA1,Nhd1
• MSU: LOC_Os08g06110
• RAPdb: Os08g0157600

• Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis, 2020, EMBO J ..
• The rice transcription factor Nhd1 regulates root growth and nitrogen uptake by activating nitrogen transporters., 2022, Plant Physiol.
• Rice CIRCADIAN CLOCK ASSOCIATED1 transcriptionally regulates ABA signaling to confer multiple abiotic stress tolerance., 2022, Plant Physiol.
• Natural alleles of CIRCADIAN CLOCK ASSOCIATED1 contribute to rice cultivation by fine-tuning flowering time., 2022, Plant Physiol.
• Rice circadian clock regulator Nhd1 controls the expression of the sucrose transporter gene OsSUT1 and impacts carbon-nitrogen balance., 2022, J Exp Bot.
• Multi-omics analyses reveal the crosstalk between the circadian clock and the response to herbicide application in Oryza sativa., 2023, Front Plant Sci.

Genbank accession number

• The rice transcription factor Nhd1 regulates root growth and nitrogen uptake by activating nitrogen transporters.
• Compared to the wild-type (WT), nhd1 and osamt1;3 mutants showed a similar decrease in root growth and N uptake under low NH4+ supply, while nhd1 and osnrt2
• The defects of nhd1 mutants in NH4+ uptake and root growth response to various N supplies were restored by overexpression of OsAMT1;3 or OsNRT2
• However, when grown in a paddy field with low N availability, nhd1 mutants accumulated more N and achieved a higher N uptake efficiency (NUpE) due to the delayed flowering time and prolonged growth period
• Here, we show that Nhd1 can directly activate the transcription of the high-affinity ammonium (NH4+) transporter 1;3 (OsAMT1;3) and the dual affinity nitrate (NO3-) transporter 2
• Together, our findings illustrate that OsCCA1 confers multiple abiotic stress tolerance likely by orchestrating ABA signaling, which links the circadian clock with ABA signaling in rice
• Consistently, oscca1 null mutants generated via genome editing displayed enhanced sensitivities to ABA signaling
• Taken together, our results demonstrate that OsCCA1 is a likely domestication locus that has contributed to the geographic adaptation and expansion of cultivated rice
• In the japonica cultivar 'Dongjin', a T-DNA insertion in OsCCA1a resulted in late flowering under long-day and short-day conditions, indicating that OsCCA1 is a floral inducer
• Leaf RNA-seq analysis indicated that mutation of Nhd1 dramatically altered expression of the genes linked to starch and sucrose metabolism, circadian rhythm, and amino acid metabolic pathways
• Knockout of Nhd1 suppressed OsSUT1 expression, and both nhd1 and ossut1 mutants showed similar shorter height, and lower shoot biomass and sucrose concentration in comparison with the wild type, while overexpression of OsSUT1 can restore the defective sucrose transport and partially ameliorate the reduced growth of nhd1 mutants
• Here, we report that knockout of Nhd1 in rice reduced the rate of photosynthesis and the sucrose ratio of sheaths to blades, but increased the total C to N ratio and free amino acids
• Rice circadian clock regulator Nhd1 controls the expression of the sucrose transporter gene OsSUT1 and impacts carbon-nitrogen balance.
• We identified that Nhd1 can directly activate the transcriptional expression of sucrose transporter-1 (OsSUT1)
• Interestingly, we identified significant OsCCA1 binding peaks in the promoter regions of four herbicide resistance genes, including OsCYP81A12, OsCYP81E22, OsCYP76C2, and OsCYP76C4

• OsCCA1~Nhd1, OsHUB2~FRRP1, OsHUB2 inhibits function of OsTrx1 in heading date in rice., The expression of Hd3a, RFT1, and Ehd1 was induced and the transcript levels of Hd1, Ghd7, OsCCA1, OsGI, OsFKF1, and OsTOC1 were reduced under long-day conditions, whereas RFT1 and Ehd1 expression was induced in oshub2 mutants under short-day conditions
• OsAMT1;3~OsAMT1.3, OsCCA1~Nhd1, The rice transcription factor Nhd1 regulates root growth and nitrogen uptake by activating nitrogen transporters., Here, we show that Nhd1 can directly activate the transcription of the high-affinity ammonium (NH4+) transporter 1;3 (OsAMT1;3) and the dual affinity nitrate (NO3-) transporter 2
• OsAMT1;3~OsAMT1.3, OsCCA1~Nhd1, The rice transcription factor Nhd1 regulates root growth and nitrogen uptake by activating nitrogen transporters., Compared to the wild-type (WT), nhd1 and osamt1;3 mutants showed a similar decrease in root growth and N uptake under low NH4+ supply, while nhd1 and osnrt2
• OsAMT1;3~OsAMT1.3, OsCCA1~Nhd1, The rice transcription factor Nhd1 regulates root growth and nitrogen uptake by activating nitrogen transporters., The defects of nhd1 mutants in NH4+ uptake and root growth response to various N supplies were restored by overexpression of OsAMT1;3 or OsNRT2
• OsCCA1~Nhd1, OsNRT2.4, The rice transcription factor Nhd1 regulates root growth and nitrogen uptake by activating nitrogen transporters, Here, we show that Nhd1 can directly activate the transcription of the high-affinity ammonium (NH4+) transporter 1;3 (OsAMT1;3) and the dual affinity nitrate (NO3-) transporter 2.4 (OsNRT2.4).
• OsAREB8~OsAREB1~OsbZIP46~OsABF2~ABL1, OsCCA1~Nhd1, Rice CIRCADIAN CLOCK ASSOCIATED1 transcriptionally regulates ABA signaling to confer multiple abiotic stress tolerance., Moreover, OsCCA1 could directly bind the promoters of OsPP108 and OsbZIP46 to activate their expression
• OsCCA1~Nhd1, OsSIPP2C1~OsPP2C68~OsPP108, Rice CIRCADIAN CLOCK ASSOCIATED1 transcriptionally regulates ABA signaling to confer multiple abiotic stress tolerance., Moreover, OsCCA1 could directly bind the promoters of OsPP108 and OsbZIP46 to activate their expression
• Ghd7.1~Hd2~OsPRR37~DTH7, OsCCA1~Nhd1, Natural alleles of CIRCADIAN CLOCK ASSOCIATED1 contribute to rice cultivation by fine-tuning flowering time., Binding affinity assays indicated that OsCCA1 binds to the promoter regions of OsPRR37 and DTH8
• Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsCCA1~Nhd1, Natural alleles of CIRCADIAN CLOCK ASSOCIATED1 contribute to rice cultivation by fine-tuning flowering time., Binding affinity assays indicated that OsCCA1 binds to the promoter regions of OsPRR37 and DTH8
• OsCCA1~Nhd1, OsSUT1, Rice circadian clock regulator Nhd1 controls the expression of the sucrose transporter gene OsSUT1 and impacts carbon-nitrogen balance., Rice circadian clock regulator Nhd1 controls the expression of the sucrose transporter gene OsSUT1 and impacts carbon-nitrogen balance.
• OsCCA1~Nhd1, OsSUT1, Rice circadian clock regulator Nhd1 controls the expression of the sucrose transporter gene OsSUT1 and impacts carbon-nitrogen balance., We identified that Nhd1 can directly activate the transcriptional expression of sucrose transporter-1 (OsSUT1)
• OsCCA1~Nhd1, OsSUT1, Rice circadian clock regulator Nhd1 controls the expression of the sucrose transporter gene OsSUT1 and impacts carbon-nitrogen balance., Knockout of Nhd1 suppressed OsSUT1 expression, and both nhd1 and ossut1 mutants showed similar shorter height, and lower shoot biomass and sucrose concentration in comparison with the wild type, while overexpression of OsSUT1 can restore the defective sucrose transport and partially ameliorate the reduced growth of nhd1 mutants
• OsCCA1~Nhd1, OsSUT1, Rice circadian clock regulator Nhd1 controls the expression of the sucrose transporter gene OsSUT1 and impacts carbon-nitrogen balance., The positively related variation of Nhd1 and OsSUT1 expression among randomly selected indica and japonica varieties suggests a common regulatory module of Nhd1-OsSUT1-mediated C and N balance in rice