Hd5,DTH8,Ghd8,OsHAP3H,LHD1,EF8,CAR8,OsNF-YB11

Information
- Symbol: Hd5,DTH8,Ghd8,OsHAP3H,LHD1,EF8,CAR8,OsNF-YB11
- MSU: LOC_Os08g07740
- RAPdb: Os08g0174500
Publication
- Genetic interactions involved in the inhibition of heading by heading date QTL, Hd2 in rice under long-day conditions, 2011, Theor Appl Genet.
- Roles of the Hd5 gene controlling heading date for adaptation to the northern limits of rice cultivation, 2013, Theor Appl Genet.
- Identification, characterization and interaction of HAP family genes in rice, 2008, Mol Genet Genomics.
- LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice Oryza rufipogon, 2012, J Integr Plant Biol.
- A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice, 2011, Mol Plant.
- DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously, 2010, Plant Physiol.
- Hd16, a gene for casein kinase I, is involved in the control of rice flowering time by modulating the day-length response, 2013, Plant J.
- Quantitative trait loci for panicle size, heading date and plant height co-segregating in trait-performance derived near-isogenic lines of rice Oryza sativa, 2006, Theor Appl Genet.
- EF8 is involved in photoperiodic flowering pathway and chlorophyll biogenesis in rice., 2014, Plant Cell Rep.
- Fine Mapping of Carbon Assimilation Rate 8, a Quantitative Trait Locus for Flag Leaf Nitrogen Content, Stomatal Conductance and Photosynthesis in Rice., 2017, Front Plant Sci.
- The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., 2017, Mol Plant.
- A key variant in the cis-regulatory element of flowering gene Ghd8 associated with cold tolerance in rice., 2019, Sci Rep.
- Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., 2019, BMC Plant Biol.
- Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, 2020, New Phytol.
- Transcriptional and post-transcriptional regulation of heading date in rice, 2020, New Phytol.
- OrMKK3 Influences Morphology and Grain Size in Rice, 2021, J Plant Biol.
- Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., 2022, J Genet Genomics.
- DTH8 overexpression induces early flowering, boosts yield, and improves stress recovery in rice cv IR64., 2022, Physiol Plant.
- The amino acid residue E96 orf Ghd8 is crucial for the formation of the flowering repression complex Ghd7-Ghd8-OsHAP5C in rice., 2022, J Integr Plant Biol.
Genbank accession number
- AB288039
Key message
- Map-based cloning reveals that DTH8 encodes a putative HAP3 subunit of the CCAAT-box-binding transcription factor and the complementary experiment increased significantly days to heading, plant height, and number of grains per panicle in CSSL61 (a chromosome segment substitution line that carries the nonfunctional DTH8 allele) with the Asominori functional DTH8 allele under long-day conditions
- Here, we report the cloning and characterization of Ghd8, a major QTL with pleiotropic effects on grain yield, heading date, and plant height
- Here, through map-based cloning, we identified a major quantitative trait loci (QTL) LHD1 (Late Heading Date 1), an allele of DTH8/Ghd8, which controls the late heading date of wild rice and encodes a putative HAP3/NF-YB/CBF-A subunit of the CCAAT-box-binding transcription factor
- LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice (Oryza rufipogon)
- Taken together, these data indicate that DTH8 probably plays an important role in the signal network of photoperiodic flowering as a novel suppressor as well as in the regulation of plant height and yield potential
- DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously
- A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice
- Meanwhile, the transcription of DTH8 has been proved to be independent of Ghd7 and Hd1, and the natural mutation of this gene caused weak photoperiod sensitivity and shorter plant height
- Ghd8 up-regulated MOC1, a key gene controlling tillering and branching; this increased the number of tillers, primary and secondary branches, thus producing 50% more grains per plant
- Our results demonstrated the important roles of Ghd8 in rice yield formation and flowering, as well as its opposite functions in flowering between rice and Arabidopsis under long-day conditions
- The Hd5 gene controlling heading date (flowering time) generated variations in heading date among cultivars adapted to Hokkaido, where is the northernmost region of Japan and one of the northern limits of rice cultivation in the world
- By regulating Ehd1, RFT1, and Hd3a, Ghd8 delayed flowering under long-day conditions, but promoted flowering under short-day conditions
- The ectopic expression of Ghd8 in Arabidopsis caused early flowering by 10 d-a situation similar to the one observed by its homolog AtHAP3b, when compared to wild-type under long-day conditions; these findings indicate the conserved function of Ghd8 and AtHAP3b in flowering in Arabidopsis
- The quantitative real-time PCR assay revealed that DTH8 could down-regulate the transcriptions of Ehd1 (for Early heading date1) and Hd3a (for Heading date3a; a rice ortholog of FLOWERING LOCUS T) under long-day conditions
- By using near-isogenic lines with functional or deficient alleles of several rice flowering-time genes, we observed significant digenetic interactions between Hd16 and four other flowering-time genes (Ghd7, Hd1, DTH8 and Hd2)
- We also found that LHD1 could down-regulate the expression of several floral transition activators such as Ehd1, Hd3a and RFT1 under long-day conditions, but not under short-day conditions
- The association of the Hd5 genotype with heading date and genetical analysis clearly showed that the loss-of-function Hd5 has an important role in exhibiting earlier heading among a local population in Hokkaido
- These results demonstrated that Hd5 plays roles not only in generating early heading in variations of heading date among a local population in Hokkaido, but also in extremely early heading for adaptation to northern limits of rice cultivation
- Roles of the Hd5 gene controlling heading date for adaptation to the northern limits of rice cultivation
- This indicates that LHD1 may delay flowering by repressing the expression of Ehd1, Hd3a and RFT1 under long-day conditions
- Sequence analysis revealed that several variants in the coding region of LHD1 were correlated with a late heading date, and a further complementary study successfully rescued the phenotype
- Distinct distribution of the loss-of-function Hd5 revealed that this mutation event of the 19-bp deletion occurred in a local landrace Bouzu and that this mutation may have been selected as an early-heading variety in rice breeding programs in Hokkaido in the early 1900s
- Our data indicate that EF8 plays an important role in rice photoperiodic flowering pathway as well as yield potential and chlorophyll biogenesis and will be an important target for rice breeding programs
- EF8 encodes a putative HAP3 subunit of the CCAAT-box-binding transcription factor, which is localized to the nucleus
- EF8 is involved in photoperiodic flowering pathway and chlorophyll biogenesis in rice
- Fine mapping suggested that CAR8 encodes a putative Heme Activator Protein 3 (OsHAP3) subunit of a CCAAT-box-binding transcription factor called OsHAP3H
- This indicates that CAR8 affects multiple physiological aspects relating to photosynthesis
- The detailed analysis of molecular functions of CAR8 would help to understand the association between photosynthesis and flowering and demonstrate specific genetic mechanisms that can be exploited to improve photosynthesis in rice and potentially other crops
- A key variant in the cis-regulatory element of flowering gene Ghd8 associated with cold tolerance in rice.
- Transgenic analyses revealed that higher expression levels of Ghd8 delayed heading date and enhanced cold tolerance in rice
- Ghd8 and Ghd7, two major flowering genes, have similar functions and large pleiotropic effects in controlling the heading date, plant height and grain yield of rice
- The results of this study help to elucidate the genetic and molecular bases of Ghd8 and Ghd7 interactions, indicating that Ghd8 acts upstream of Ghd7 to activate its transcription, which inhibits Hd3a expression and thus affects flowering time and rice adaptation
- However, under LD conditions, Hd1 promotes Ghd7 expression and is recruited by Ghd7 and/or DTH8 to form repressive complexes that collaboratively suppress the Ehd1-Hd3a/RFT1 pathway to block heading, but under SD conditions Hd1 competes with the complexes to promote Hd3a/RFT1 expression, playing a tradeoff relationship with PS flowering
- Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits.
- In this study, we systematically analyze the heading date, PS, and agronomic traits of eight homozygous lines with various combinations of Hd1, Ghd7, and DTH8 alleles in the prr37 background under long-day (LD) and short-day (SD) conditions, respectively
- DTH8 up-regulates the transcription of RFT1, Hd3a, GHD7, MOC1, and RCN1 in IR64 at the pre-flowering stage and plays a role in early flowering, increased number of tillers, enhanced panicle branching, and improved tolerance towards drought and salinity stress at the reproductive stage
- Taken together, DTH8 is a positive regulator of the network of genes related to early flowering/heading, higher yield, as well as salinity and drought stress tolerance, thus, enabling the crops to adapt to a wide range of climatic conditions
- We demonstrate DTH8 to be positively influencing the yield, heading date, and stress tolerance in IR64
- In addition, DTH8 overexpressing transgenic lines showed favorable physiological parameters causing less yield penalty under stress than the WT plants
- DTH8 overexpression induces early flowering, boosts yield, and improves stress recovery in rice cv IR64.
- The amino acid residue E96 orf Ghd8 is crucial for the formation of the flowering repression complex Ghd7-Ghd8-OsHAP5C in rice.
- MutMap analysis revealed that SOG7 is allelic to Ghd8 and delayed flowering under long-day (LD) conditions
Connection
- CKI~EL1~Hd16, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Genetic interactions involved in the inhibition of heading by heading date QTL, Hd2 in rice under long-day conditions, Combinations of several QTLs near Hd1, Hd2, Ghd7, Hd5, and Hd16 were detected under these four conditions
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Genetic interactions involved in the inhibition of heading by heading date QTL, Hd2 in rice under long-day conditions, Combinations of several QTLs near Hd1, Hd2, Ghd7, Hd5, and Hd16 were detected under these four conditions
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Genetic interactions involved in the inhibition of heading by heading date QTL, Hd2 in rice under long-day conditions, Combinations of several QTLs near Hd1, Hd2, Ghd7, Hd5, and Hd16 were detected under these four conditions
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Roles of the Hd5 gene controlling heading date for adaptation to the northern limits of rice cultivation, The loss-of-function Hd5 was then introduced into the rice variety Fanny from France and contributed to its extremely early heading under the presence of functional Ghd7
- Ehd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice Oryza rufipogon, We also found that LHD1 could down-regulate the expression of several floral transition activators such as Ehd1, Hd3a and RFT1 under long-day conditions, but not under short-day conditions
- Ehd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice Oryza rufipogon, This indicates that LHD1 may delay flowering by repressing the expression of Ehd1, Hd3a and RFT1 under long-day conditions
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsGI-Hd1-Hd3a~RFT1, LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice Oryza rufipogon, We also found that LHD1 could down-regulate the expression of several floral transition activators such as Ehd1, Hd3a and RFT1 under long-day conditions, but not under short-day conditions
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsGI-Hd1-Hd3a~RFT1, LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice Oryza rufipogon, This indicates that LHD1 may delay flowering by repressing the expression of Ehd1, Hd3a and RFT1 under long-day conditions
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice Oryza rufipogon, We also found that LHD1 could down-regulate the expression of several floral transition activators such as Ehd1, Hd3a and RFT1 under long-day conditions, but not under short-day conditions
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, LHD1, an allele of DTH8/Ghd8, controls late heading date in common wild rice Oryza rufipogon, This indicates that LHD1 may delay flowering by repressing the expression of Ehd1, Hd3a and RFT1 under long-day conditions
- Ehd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice, By regulating Ehd1, RFT1, and Hd3a, Ghd8 delayed flowering under long-day conditions, but promoted flowering under short-day conditions
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice, By regulating Ehd1, RFT1, and Hd3a, Ghd8 delayed flowering under long-day conditions, but promoted flowering under short-day conditions
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, MOC1, A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice, Ghd8 up-regulated MOC1, a key gene controlling tillering and branching; this increased the number of tillers, primary and secondary branches, thus producing 50% more grains per plant
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsGI-Hd1-Hd3a~RFT1, A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice, By regulating Ehd1, RFT1, and Hd3a, Ghd8 delayed flowering under long-day conditions, but promoted flowering under short-day conditions
- Ehd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously, The quantitative real-time PCR assay revealed that DTH8 could down-regulate the transcriptions of Ehd1 (for Early heading date1) and Hd3a (for Heading date3a; a rice ortholog of FLOWERING LOCUS T) under long-day conditions
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously, Meanwhile, the transcription of DTH8 has been proved to be independent of Ghd7 and Hd1, and the natural mutation of this gene caused weak photoperiod sensitivity and shorter plant height
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously, Meanwhile, the transcription of DTH8 has been proved to be independent of Ghd7 and Hd1, and the natural mutation of this gene caused weak photoperiod sensitivity and shorter plant height
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously, The quantitative real-time PCR assay revealed that DTH8 could down-regulate the transcriptions of Ehd1 (for Early heading date1) and Hd3a (for Heading date3a; a rice ortholog of FLOWERING LOCUS T) under long-day conditions
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd16, a gene for casein kinase I, is involved in the control of rice flowering time by modulating the day-length response, By using near-isogenic lines with functional or deficient alleles of several rice flowering-time genes, we observed significant digenetic interactions between Hd16 and four other flowering-time genes (Ghd7, Hd1, DTH8 and Hd2)
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd16, a gene for casein kinase I, is involved in the control of rice flowering time by modulating the day-length response, By using near-isogenic lines with functional or deficient alleles of several rice flowering-time genes, we observed significant digenetic interactions between Hd16 and four other flowering-time genes (Ghd7, Hd1, DTH8 and Hd2)
- CKI~EL1~Hd16, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd16, a gene for casein kinase I, is involved in the control of rice flowering time by modulating the day-length response, By using near-isogenic lines with functional or deficient alleles of several rice flowering-time genes, we observed significant digenetic interactions between Hd16 and four other flowering-time genes (Ghd7, Hd1, DTH8 and Hd2)
- Ghd7.1~Hd2~OsPRR37~DTH7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Days to heading 7, a major quantitative locus determining photoperiod sensitivity and regional adaptation in rice., Further, we find that haplotype combinations of DTH7 with Grain number, plant height, and heading date 7 (Ghd7) and DTH8 correlate well with the heading date and grain yield of rice under different photoperiod conditions
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, EF8 is involved in photoperiodic flowering pathway and chlorophyll biogenesis in rice., Our data showed that EF8 delayed flowering time under long-day conditions by altering the rhythmic expression patterns of ‘florigen’ genes Hd3a and RFT1
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsGI-Hd1-Hd3a~RFT1, EF8 is involved in photoperiodic flowering pathway and chlorophyll biogenesis in rice., Our data showed that EF8 delayed flowering time under long-day conditions by altering the rhythmic expression patterns of ‘florigen’ genes Hd3a and RFT1
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Combinations of the Ghd7, Ghd8 and Hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice., Combinations of the Ghd7, Ghd8 and Hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice.
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Combinations of the Ghd7, Ghd8 and Hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice., We employed a map-based cloning approach to isolate a heading date gene, which coordinated the interaction between Ghd7 and Ghd8 to greatly delay rice heading
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Combinations of the Ghd7, Ghd8 and Hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice., Map-based cloning demonstrated that the gene largely affecting the interaction between Ghd7 and Ghd8 was Hd1
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Combinations of the Ghd7, Ghd8 and Hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice., Natural variation analysis showed that a combination of loss-of-function alleles of Ghd7, Ghd8 and Hd1 contributes to the expansion of rice cultivars to higher latitudes; by contrast, a combination of pre-existing strong alleles of Ghd7, Ghd8 and functional Hd1 (referred as SSF) is exclusively found where ancestral Asian cultivars originated
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Combinations of the Ghd7, Ghd8 and Hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice., Our results indicate that the combinations of Ghd7, Ghd8 and Hd1 largely define the ecogeographical adaptation and yield potential in rice cultivars
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsHAPL1, The OsHAPL1-DTH8-Hd1 complex functions as the transcription regulator to repress heading date in rice., OsHAPL1 can physically interact with Days To Heading on chromosome 8 (DTH8), which physically interacts with Heading date 1 (Hd1) both in vitro and in vivo OsHAPL1 forms a complex with DTH8 and Hd1 in Escherichia coli OsHAPL1, DTH8, and Hd1 physically interact with the HAP complex, and also with general transcription factors in yeast (Saccharomyces cerevisiae)
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsHAPL1, The OsHAPL1-DTH8-Hd1 complex functions as the transcription regulator to repress heading date in rice., We propose that OsHAPL1 functions as a transcriptional regulator and, together with DTH8, Hd1, the HAP complex, and general transcription factors, regulates the expression of target genes and then affects heading date by influencing the expression of Hd3a and RFT1 through Ehd1
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Transcriptional and Post-transcriptional Mechanisms Limit Heading Date 1 Hd1 Function to Adapt Rice to High Latitudes., We demonstrate that a histone fold domain scaffold formed by GRAIN YIELD, PLANT HEIGHT AND HEADING DATE 8 (Ghd8) and several NF-YC subunits can accommodate distinct proteins, including Hd1 and PSEUDO RESPONSE REGULATOR 37 (PRR37), and that the resulting OsNF-Y complex containing Hd1 can bind a specific sequence in the promoter of HEADING DATE 3A (Hd3a)
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., Here, we demonstrate that the repression of flowering in LD mediated by Hd1 is dependent on the transcription factor DAYS TO HEADING 8 (DTH8)
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., Loss of DTH8 function results in the activation of Hd3a by Hd1, leading to early flowering
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., We also show that Hd1 directly interacts with DTH8 and that the formation of the DTH8-Hd1 complex is necessary for the transcriptional repression of Hd3a by Hd1 in LD
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., We thus detected a switch in function of Hd1 mediated by DTH8 in LD rather than in SD
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., H3K27 trimethylation increases at Hd3a in the presence the DTH8-Hd1 complex, while Hd1 attenuates the H3K27me3 level in Hd3a when DTH8 function is lost
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., Therefore, our findings establish that, in response to day length, DTH8 plays a critical role in mediating Hd1 regulation of Hd3a transcription through the DTH8-Hd1 module to shape epigenetic marks in photoperiodic flowering
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., Loss of DTH8 function results in the activation of Hd3a by Hd1, leading to early flowering
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., We also show that Hd1 directly interacts with DTH8 and that the formation of the DTH8-Hd1 complex is necessary for the transcriptional repression of Hd3a by Hd1 in LD
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., Further, we reveal that DTH8 associates with the Hd3a promoter to modulate the level of trimethylated H3K27 at the Hd3a genomic locus
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., H3K27 trimethylation increases at Hd3a in the presence the DTH8-Hd1 complex, while Hd1 attenuates the H3K27me3 level in Hd3a when DTH8 function is lost
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The DTH8-Hd1 module mediates day length-dependent regulation of rice flowering., Therefore, our findings establish that, in response to day length, DTH8 plays a critical role in mediating Hd1 regulation of Hd3a transcription through the DTH8-Hd1 module to shape epigenetic marks in photoperiodic flowering
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Loss-of-Function Alleles of Heading date 1 Hd1 Are Associated With Adaptation of Temperate Japonica Rice Plants to the Tropical Region., We analyzed the 45 diverse rice accessions and 12 tropical-adapted temperate japonica lines for the allele types of seven major flowering genes Hd1, OsPPR37, DTH8, Ghd7, Ehd1, RFT1, and Hd3a and flowering time under three different field conditions in temperate and tropical locations
- Ghd7.1~Hd2~OsPRR37~DTH7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Genetic Interactions Among Ghd7, Ghd8, OsPRR37 and Hd1 Contribute to Large Variation in Heading Date in Rice., Genetic Interactions Among Ghd7, Ghd8, OsPRR37 and Hd1 Contribute to Large Variation in Heading Date in Rice.
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7.
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., Ghd8 and Ghd7, two major flowering genes, have similar functions and large pleiotropic effects in controlling the heading date, plant height and grain yield of rice
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., In this study, we investigated the genetic interaction between Ghd8 and Ghd7 by using a set of near-isogenic lines and a panel of natural germplasm accessions in rice
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., Both functional Ghd8 and Ghd7 are pivotal for rice photoperiod sensitivity controlled by Hd1 and Hd3a
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., GHD8 could form a heterotrimeric complex with HD1 and OsHAP5b to activate the transcription of Ghd7 by binding directly to the promoter region of Ghd7, which contains the CCAAT-box motif
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., The results of this study help to elucidate the genetic and molecular bases of Ghd8 and Ghd7 interactions, indicating that Ghd8 acts upstream of Ghd7 to activate its transcription, which inhibits Hd3a expression and thus affects flowering time and rice adaptation
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., Both functional Ghd8 and Ghd7 are pivotal for rice photoperiod sensitivity controlled by Hd1 and Hd3a
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., GHD8 could form a heterotrimeric complex with HD1 and OsHAP5b to activate the transcription of Ghd7 by binding directly to the promoter region of Ghd7, which contains the CCAAT-box motif
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., Both functional Ghd8 and Ghd7 are pivotal for rice photoperiod sensitivity controlled by Hd1 and Hd3a
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7., The results of this study help to elucidate the genetic and molecular bases of Ghd8 and Ghd7 interactions, indicating that Ghd8 acts upstream of Ghd7 to activate its transcription, which inhibits Hd3a expression and thus affects flowering time and rice adaptation
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsCOL4, A Combination of Long-Day Suppressor Genes Contributes to the Northward Expansion of Rice , Here, we report the analysis of genetic variation in eight long-day flowering suppressor genes (Hd1, DTH8, Ghd7, OsCOL4, DTH7, Hd6, Se5, and PhyB) and the phylogenetic relationship of eight genes
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsCOL4, A Combination of Long-Day Suppressor Genes Contributes to the Northward Expansion of Rice , Genetic variations in DTH8, Ghd7, Hd1, DTH7, PhyB, and OsCOL4 are correlated with differences in heading date and the correlation between the genetic diversity of Hd6 and Se5 and rice heading data are weak
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsCOL4, A Combination of Long-Day Suppressor Genes Contributes to the Northward Expansion of Rice , One group of haplotypes of DTH8, Ghd7, Hd1, DTH7, PhyB, and OsCOL4 are associated with earlier heading dates and appear to have accumulated during the northward expansion of rice cultivation
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsCOL4, A Combination of Long-Day Suppressor Genes Contributes to the Northward Expansion of Rice , A minimum of four group A alleles of DTH8, Ghd7, Hd1, DTH7, PhyB, and OsCOL4 are required for the growth of cultivated rice at latitudes above 30°N
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, We created a set of isogenic lines among the core PS-flowering genes Hd1, Ghd7 and DTH8 using CRISPR mutagenesis, to systematically dissect their genetic relationships under different day-lengths
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, However, under LD conditions, Hd1 promotes Ghd7 expression and is recruited by Ghd7 and/or DTH8 to form repressive complexes that collaboratively suppress the Ehd1-Hd3a/RFT1 pathway to block heading, but under SD conditions Hd1 competes with the complexes to promote Hd3a/RFT1 expression, playing a tradeoff relationship with PS flowering
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, Natural allelic variations of Hd1, Ghd7 and DTH8 in rice populations have resulted in various PS performances
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, We created a set of isogenic lines among the core PS-flowering genes Hd1, Ghd7 and DTH8 using CRISPR mutagenesis, to systematically dissect their genetic relationships under different day-lengths
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, However, under LD conditions, Hd1 promotes Ghd7 expression and is recruited by Ghd7 and/or DTH8 to form repressive complexes that collaboratively suppress the Ehd1-Hd3a/RFT1 pathway to block heading, but under SD conditions Hd1 competes with the complexes to promote Hd3a/RFT1 expression, playing a tradeoff relationship with PS flowering
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading, Natural allelic variations of Hd1, Ghd7 and DTH8 in rice populations have resulted in various PS performances
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits.
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., The core heading date genes Hd1, Ghd7, DTH8, and PRR37 act synergistically in regulating the PS
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., In this study, we systematically analyze the heading date, PS, and agronomic traits of eight homozygous lines with various combinations of Hd1, Ghd7, and DTH8 alleles in the prr37 background under long-day (LD) and short-day (SD) conditions, respectively
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., However, under LDs, Hd1 suppresses flowering, in coordination with functional Ghd7 or with Ghd7 and DTH8
- Hd1, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., Lines carrying Ghd7 and DTH8 (with hd1) show delayed heading and improve agronomic traits
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits.
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., The core heading date genes Hd1, Ghd7, DTH8, and PRR37 act synergistically in regulating the PS
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., In this study, we systematically analyze the heading date, PS, and agronomic traits of eight homozygous lines with various combinations of Hd1, Ghd7, and DTH8 alleles in the prr37 background under long-day (LD) and short-day (SD) conditions, respectively
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., However, under LDs, Hd1 suppresses flowering, in coordination with functional Ghd7 or with Ghd7 and DTH8
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Hd1, Ghd7, and DTH8 synergistically determine the rice heading date and yield-related agronomic traits., Lines carrying Ghd7 and DTH8 (with hd1) show delayed heading and improve agronomic traits
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, DTH8 overexpression induces early flowering, boosts yield, and improves stress recovery in rice cv IR64., DTH8 up-regulates the transcription of RFT1, Hd3a, GHD7, MOC1, and RCN1 in IR64 at the pre-flowering stage and plays a role in early flowering, increased number of tillers, enhanced panicle branching, and improved tolerance towards drought and salinity stress at the reproductive stage
- Hd3a, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, DTH8 overexpression induces early flowering, boosts yield, and improves stress recovery in rice cv IR64., DTH8 up-regulates the transcription of RFT1, Hd3a, GHD7, MOC1, and RCN1 in IR64 at the pre-flowering stage and plays a role in early flowering, increased number of tillers, enhanced panicle branching, and improved tolerance towards drought and salinity stress at the reproductive stage
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, MOC1, DTH8 overexpression induces early flowering, boosts yield, and improves stress recovery in rice cv IR64., DTH8 up-regulates the transcription of RFT1, Hd3a, GHD7, MOC1, and RCN1 in IR64 at the pre-flowering stage and plays a role in early flowering, increased number of tillers, enhanced panicle branching, and improved tolerance towards drought and salinity stress at the reproductive stage
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, Rcn1~OsABCG5, DTH8 overexpression induces early flowering, boosts yield, and improves stress recovery in rice cv IR64., DTH8 up-regulates the transcription of RFT1, Hd3a, GHD7, MOC1, and RCN1 in IR64 at the pre-flowering stage and plays a role in early flowering, increased number of tillers, enhanced panicle branching, and improved tolerance towards drought and salinity stress at the reproductive stage
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, RFT1, DTH8 overexpression induces early flowering, boosts yield, and improves stress recovery in rice cv IR64., DTH8 up-regulates the transcription of RFT1, Hd3a, GHD7, MOC1, and RCN1 in IR64 at the pre-flowering stage and plays a role in early flowering, increased number of tillers, enhanced panicle branching, and improved tolerance towards drought and salinity stress at the reproductive stage
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsCCA1~Nhd1, Natural alleles of CIRCADIAN CLOCK ASSOCIATED1 contribute to rice cultivation by fine-tuning flowering time., Binding affinity assays indicated that OsCCA1 binds to the promoter regions of OsPRR37 and DTH8
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The amino acid residue E96 orf Ghd8 is crucial for the formation of the flowering repression complex Ghd7-Ghd8-OsHAP5C in rice., Biochemical assays showed that Ghd8 interacts with OsHAP5C and Ghd7 both in vivo and in vitro
- Ghd7, Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, The amino acid residue E96 orf Ghd8 is crucial for the formation of the flowering repression complex Ghd7-Ghd8-OsHAP5C in rice., Surprisingly, a point mutation E96K in 伪2 helix of Ghd8 HFD domain destroyed its ability interacting with Ghd7
- Hd5~DTH8~Ghd8~OsHAP3H~LHD1~EF8~CAR8~OsNF-YB11, OsHAP5C, The amino acid residue E96 orf Ghd8 is crucial for the formation of the flowering repression complex Ghd7-Ghd8-OsHAP5C in rice., Biochemical assays showed that Ghd8 interacts with OsHAP5C and Ghd7 both in vivo and in vitro

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