OsGAD1

2015-01-20 | Categories genes  | Tags root  seed  shoot 

• Information
  • Symbol: OsGAD1
  • MSU: LOC_Os08g36320
  • RAPdb: Os08g0465800
• Publication
  • Rice Oryza sativa contains a novel isoform of glutamate decarboxylase that lacks an authentic calmodulin-binding domain at the C-terminus, 2001, Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression.
• Genbank accession number
  • AB056062
• Key message
  • RNA analysis showed that transcripts of OsGAD1 and OsGAD2 were present in all tissues examined, but their expression was differentially regulated, at least in roots and maturing seeds
  • We have isolated full-length cDNAs for two distinct isoforms of glutamate decarboxylase (GAD), designated OsGAD1 and OsGAD2 from a rice shoot cDNA library
• Connection
  • OsGAD1, OsGAD2, Rice Oryza sativa contains a novel isoform of glutamate decarboxylase that lacks an authentic calmodulin-binding domain at the C-terminus, We have isolated full-length cDNAs for two distinct isoforms of glutamate decarboxylase (GAD), designated OsGAD1 and OsGAD2 from a rice shoot cDNA library
  • OsGAD1, OsGAD2, Rice Oryza sativa contains a novel isoform of glutamate decarboxylase that lacks an authentic calmodulin-binding domain at the C-terminus, Open reading frames found in OsGAD1 and OsGAD2 cDNAs encode putative proteins of 501 (56
  • OsGAD1, OsGAD2, Rice Oryza sativa contains a novel isoform of glutamate decarboxylase that lacks an authentic calmodulin-binding domain at the C-terminus, Interestingly, in the regions in the putative gene products corresponding to the C-terminal 30-amino-acid peptide known as the calmodulin-binding domain of plant GADs, OsGAD1 possesses a typical motif, while OsGAD2 contains several substitutions of amino acids that contribute strongly to the binding of calmodulin (CaM)
  • OsGAD1, OsGAD2, Rice Oryza sativa contains a novel isoform of glutamate decarboxylase that lacks an authentic calmodulin-binding domain at the C-terminus, An in vitro CaM-binding assay of these proteins over-expressed in Escherichia coli revealed that OsGAD1 can in fact bind specifically to bovine CaM but OsGAD2 cannot
  • OsGAD1, OsGAD2, Rice Oryza sativa contains a novel isoform of glutamate decarboxylase that lacks an authentic calmodulin-binding domain at the C-terminus, RNA analysis showed that transcripts of OsGAD1 and OsGAD2 were present in all tissues examined, but their expression was differentially regulated, at least in roots and maturing seeds

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