OsHI-LOX,OsLOX9

2015-01-20 | Categories genes  | Tags brown planthopper  ethylene  leaf  ja  chloroplast  stem 

• Information
  • Symbol: OsHI-LOX,OsLOX9
  • MSU: LOC_Os08g39840
  • RAPdb: Os08g0508800
• Publication
  • Silencing OsHI-LOX makes rice more susceptible to chewing herbivores, but enhances resistance to a phloem feeder, 2009, Plant J.
  • The chloroplast-localized phospholipases D alpha4 and alpha5 regulate herbivore-induced direct and indirect defenses in rice, 2011, Plant Physiol.
  • OsNPR1 negatively regulates herbivore-induced JA and ethylene signaling and plant resistance to a chewing herbivore in rice, 2013, Physiol Plant.
  • OsMPK3 positively regulates the JA signaling pathway and plant resistance to a chewing herbivore in rice, 2013, Plant Cell Rep.
  • Novel crosstalk between ethylene- and jasmonic acid-pathway responses to a piercing-sucking insect in rice., 2019, New Phytol.
• Genbank accession number
  • AK241395
• Key message
  • We cloned a chloroplast-localized type 2 13-lipoxygenase gene of rice, OsHI-LOX, whose transcripts were up-regulated in response to feeding by the rice striped stem borer (SSB) Chilo suppressalis and the rice brown planthopper (BPH) Niaparvata lugens, as well as by mechanical wounding and treatment with JA
  • The antisense expression of OsNPR1 (as-npr1), which reduced the expression of the gene by 50%, increased elicited levels of JA and ethylene (ET) as well as of expression of a lipoxygenase gene OsHI-LOX and an ACC synthase gene OsACS2
  • Antisense expression of OsHI-LOX (as-lox) reduced SSB- or BPH-induced JA and trypsin protease inhibitor (TrypPI) levels, improved the larval performance of SBB as well as that of the rice leaf folder (LF) Cnaphalocrocis medinalis, and increased the damage caused by SSB and LF larvae
  • These results imply that OsHI-LOX is involved in herbivore-induced JA biosynthesis, and plays contrasting roles in controlling rice resistance to chewing and phloem-feeding herbivores
  • Silencing OsMPK3 (ir-mpk3) reduced the expression of the gene by 50-70 %, decreased elicited levels of JA and diminished the expression of a lipoxygenase gene OsHI-LOX and an allene oxide synthase gene OsAOS1
  • Antisense expression of OsPLDalpha4 and -alpha5 (as-pld), which resulted in a 50% reduction of the expression of the two genes, reduced elicited levels of linolenic acid, JA, green leaf volatiles, and ethylene and attenuated the SSB-induced expression of a mitogen-activated protein kinase (OsMPK3), a lipoxygenase (OsHI-LOX), a hydroperoxide lyase (OsHPL3), as well as a 1-aminocyclopropane-1-carboxylic acid synthase (OsACS2)
• Connection
  • OsAOS1, OsHI-LOX~OsLOX9, OsMPK3 positively regulates the JA signaling pathway and plant resistance to a chewing herbivore in rice, Silencing OsMPK3 (ir-mpk3) reduced the expression of the gene by 50-70 %, decreased elicited levels of JA and diminished the expression of a lipoxygenase gene OsHI-LOX and an allene oxide synthase gene OsAOS1
  • OsACS2, OsHI-LOX~OsLOX9, The chloroplast-localized phospholipases D alpha4 and alpha5 regulate herbivore-induced direct and indirect defenses in rice, Antisense expression of OsPLDalpha4 and -alpha5 (as-pld), which resulted in a 50% reduction of the expression of the two genes, reduced elicited levels of linolenic acid, JA, green leaf volatiles, and ethylene and attenuated the SSB-induced expression of a mitogen-activated protein kinase (OsMPK3), a lipoxygenase (OsHI-LOX), a hydroperoxide lyase (OsHPL3), as well as a 1-aminocyclopropane-1-carboxylic acid synthase (OsACS2)
  • OsHI-LOX~OsLOX9, OsHPL3, The chloroplast-localized phospholipases D alpha4 and alpha5 regulate herbivore-induced direct and indirect defenses in rice, Antisense expression of OsPLDalpha4 and -alpha5 (as-pld), which resulted in a 50% reduction of the expression of the two genes, reduced elicited levels of linolenic acid, JA, green leaf volatiles, and ethylene and attenuated the SSB-induced expression of a mitogen-activated protein kinase (OsMPK3), a lipoxygenase (OsHI-LOX), a hydroperoxide lyase (OsHPL3), as well as a 1-aminocyclopropane-1-carboxylic acid synthase (OsACS2)
  • OsACS2, OsHI-LOX~OsLOX9, OsNPR1 negatively regulates herbivore-induced JA and ethylene signaling and plant resistance to a chewing herbivore in rice, The antisense expression of OsNPR1 (as-npr1), which reduced the expression of the gene by 50%, increased elicited levels of JA and ethylene (ET) as well as of expression of a lipoxygenase gene OsHI-LOX and an ACC synthase gene OsACS2
  • OsHI-LOX~OsLOX9, OsNPR1~NH1, OsNPR1 negatively regulates herbivore-induced JA and ethylene signaling and plant resistance to a chewing herbivore in rice, The antisense expression of OsNPR1 (as-npr1), which reduced the expression of the gene by 50%, increased elicited levels of JA and ethylene (ET) as well as of expression of a lipoxygenase gene OsHI-LOX and an ACC synthase gene OsACS2
  • OsEIL1~MHZ6, OsHI-LOX~OsLOX9, Novel crosstalk between ethylene- and jasmonic acid-pathway responses to a piercing-sucking insect in rice., An RNA-seq analysis revealed that a JA biosynthetic pathway-related gene, OsLOX9, was down-regulated significantly in the oseil1 mutant
  • OsEIL1~MHZ6, OsHI-LOX~OsLOX9, Novel crosstalk between ethylene- and jasmonic acid-pathway responses to a piercing-sucking insect in rice., Biochemical analyses including yeast one-hybrid, Dual-LUC (Dual luciferase) and EMSA (Electrophoretic mobility shift assay) confirmed the direct regulation of OsLOX9 by OsEIL1
  • OsEIL1~MHZ6, OsHI-LOX~OsLOX9, Novel crosstalk between ethylene- and jasmonic acid-pathway responses to a piercing-sucking insect in rice., The synergistic mechanism was realized by transcriptional regulation of OsEIL1 on OsLOX9

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