- Information
- Symbol: Os8N3,xa13,OsSWEET11
- MSU: LOC_Os08g42350
- RAPdb: Os08g0535200
- Publication
- Targeting xa13, a recessive gene for bacterial blight resistance in rice, 2006, Theor Appl Genet.
- Genetic and physical mapping of xa13 , a recessive bacterial blight resistance gene in rice, 1999, TAG Theoretical and Applied Genetics.
- Gene silencing using the recessive rice bacterial blight resistance gene xa13 as a new paradigm in plant breeding, 2012, Plant Cell Rep.
- Promoter mutations of an essential gene for pollen development result in disease resistance in rice, 2006, Genes Dev.
- Transcription activator-like TAL effectors targeting OsSWEET genes enhance virulence on diverse rice Oryza sativa varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae, 2012, New Phytol.
- Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3, 2010, Plant Cell.
- Os8N3 is a host disease-susceptibility gene for bacterial blight of rice, 2006, Proc Natl Acad Sci U S A.
- Characterization of Xanthomonas oryzae-responsive cis-acting element in the promoter of rice race-specific susceptibility gene Xa13, 2011, Mol Plant.
- Identification of Resistance Genes Effective Against Rice Bacterial Blight Pathogen in Eastern India, 2001, Plant Disease.
- Essential Role of Sugar Transporter OsSWEET11 during the Early Stage of Rice Grain Filling., 2017, Plant Cell Physiol.
- SWEET11 and 15 as key players in seed filling in rice., 2018, New Phytol.
- Inhibition of OsSWEET11 function in mesophyll cells improves resistance of rice to sheath blight disease., 2018, Mol Plant Pathol.
- Transcriptome Analysis and Functional Identification of Xa13 and Pi-ta Orthologs in Oryza granulata., 2018, Plant Genome.
- CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae., 2019, Rice (N Y).
- Broad-spectrum resistance to bacterial blight in rice using genome editing., 2019, Nat Biotechnol.
- Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, 2020, Front Plant Sci.
- Gene Expression Dynamics in Rice Peduncles at the Heading Stage, 2020, Front Genet.
- OsRRM, an RNA-Binding Protein, Modulates Sugar Transport in Rice Oryza sativa L., 2020, Front Plant Sci.
- OsSWEET14 cooperates with OsSWEET11 to contribute to grain filling in rice, 2021, Plant Sci.
- Knockout of OsSWEET15 Impairs Rice Embryo Formation and Seed-Setting., 2022, Plant Cell Physiol.
- Genbank accession number
- Key message
- Here we show that xa13, a recessive allele conferring disease resistance against bacterial blight, one of the most devastating rice diseases worldwide, plays a key role in both disease resistance and pollen development
- The dominant allele, Xa13, is required for both bacterial growth and pollen development
- The findings indicate that Os8N3 is a host susceptibility gene for bacterial blight targeted by the type III effector PthXo1
- Os8N3 is a host disease-susceptibility gene for bacterial blight of rice
- The recessive gene, xa13, confers resistance to Philippine race 6 (PXO99) of the bacterial blight pathogen Xanthomonas oryzae pv oryzae
- Genetic and physical mapping of xa13 , a recessive bacterial blight resistance gene in rice
- These markers will be useful tools for the marker-assisted selection of xa13 in breeding programs
- The recessive rice bacterial blight resistance gene xa13, also involved in pollen development, has been cloned and its resistance mechanism has been recently characterized
- This report describes the conversion of bacterial blight resistance mediated by the recessive xa13 gene into a dominant trait to facilitate its use in a breeding program
- Gene silencing using the recessive rice bacterial blight resistance gene xa13 as a new paradigm in plant breeding
- To clone and characterize the recessive R genes, we fine-mapped xa13, a fully recessive gene for Xoo resistance, to a DNA fragment of 14
- Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3
- Targeting xa13, a recessive gene for bacterial blight resistance in rice
- Tissue-specific promoters were used to exclude most of the expression of artificial microRNA in the anther to ensure that Xa13 functioned normally during pollen development
- Representative pathotypes were used to evaluate seven near-isogenic lines carrying individual bacterial blight resistance genes (Xa3, Xa4, xa5, Xa7, Xa10, xa13, and Xa21) and gene pyramids
- Silencing of Os8N3 by inhibitory RNA produced plants that were resistant to infection by strain PXO99(A) yet remained susceptible to other strains of the pathogen
- PXO99 causes rice disease by inducing Xa13
- The recessive xa13 allele represents a new type of plant disease resistance
- Essential Role of Sugar Transporter OsSWEET11 during the Early Stage of Rice Grain Filling.
- The knockout of OsSWEET11 significantly decreased sucrose concentration in the mutant embryo-sacs and led to defective grain filling compared with that of the wild-type (WT) plant
- This study investigated the role of sugar transporter OsSWEET11 during the early stage of rice caryopsis development using the β-glucoronidase (GUS)-represented expression, CRISPR-Cas9-mediated knockout, cross-fertilization, and RNA-seq analyses
- These results strongly suggested that OsSWEET11 played an important role in sucrose release from maternal tissue to the maternal-filial interface during the early stage of caryopsis development
- Inhibition of OsSWEET11 function in mesophyll cells improves resistance of rice to sheath blight disease.
- In this study, we found that the rice sugar transporter, OsSWEET11 is involved in the pathogenesis of sheath blight disease
- The analyses of transgenic plants revealed that Ossweet11 mutants were less susceptible, whereas plants overexpressing OsSWEET11 were more susceptible to sheath blight compared to wild-type controls, but the yield of OsSWEET11 mutants and overexpressors was reduced
- To analyze whether inhibition of OsSWEET11 function in mesophyll cells is related to defense against this disease, mutated- OsSWEET11 was expressed under the control of Rubisco promoter, which is specific for green tissues
- The plants can be protected from infection by manipulating the expression of OsSWEET11 without affecting the crop yield
- solani might acquire sugar from rice leaves by activating OsSWEET11 expression
- CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae.
- It has previously been reported that, in rice plants, knockdown of the Os8N3 gene resulted in enhanced resistance to Xanthomonas oryzae pv
- Analysis of the genotypes and edited Os8N3 in T0, T1, T2, and T3 transgenic rice plants showed that the mutations were transmitted to subsequent generations, and homozygous mutants displayed significantly enhanced resistance to Xoo
- Homozygous (BC3F3 generation), three-gene bacterial blight pyramided (xa5 + xa13 + Xa21) lines were developed, and these lines were crossed with Tetep to combine blast (Pi54) and sheath blight (qSBR7-1, qSBR11-1, and qSBR11-2) resistance
- In BC3F3 generation, the improved pyramided lines carrying a total of seven genes/QTLs (xa5 + xa13 + Xa21 + Pi54 + qSBR7-1 + qSBR11-1 + qSBR11-2) were selected through molecular and phenotypic assay, and these were evaluated for resistance against bacterial blight, blast, and sheath blight pathogens under greenhouse conditions
- Both OsSWEET14 and OsSWEET11 exhibited distinct spatiotemporal expression patterns between the early stage of caryopsis development and the rapid grain-filling stage
- OsSWEET14 cooperates with OsSWEET11 to contribute to grain filling in rice
- ossweet14 knockout mutants did not show any detectable phenotypic differences from the wild type, whereas ossweet14;ossweet11 double-knockout mutants had much more severe phenotypes than ossweet11 single-knockout mutants, including strongly reduced grain weight and yield, reduced grain-filling rate, and increased starch accumulation in the pericarp
- During the rapid grain-filling stage, OsSWEET14 and OsSWEET11 localized to four key sites: vascular parenchyma cells, the nucellar projection, the nucellar epidermis, and cross cells
- These results indicate that both OsSWEET15 and OsSWEET11 play important and similar roles in rice pollen development, caryopsis formation and seed-setting, in addition to their function in seed-filling that was demonstrated previously
- Connection
- Os8N3~xa13~OsSWEET11, xa21, Identification of Resistance Genes Effective Against Rice Bacterial Blight Pathogen in Eastern India, Representative pathotypes were used to evaluate seven near-isogenic lines carrying individual bacterial blight resistance genes (Xa3, Xa4, xa5, Xa7, Xa10, xa13, and Xa21) and gene pyramids
- Os11N3~OsSWEET14, Os8N3~xa13~OsSWEET11, Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3, The finding that Os-8N3 and Os-11N3 encode closely related proteins also provides evidence that N3 proteins have a specific function in facilitating bacterial blight disease
- Os11N3~OsSWEET14, Os8N3~xa13~OsSWEET11, Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3, Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3
- Os8N3~xa13~OsSWEET11, OsSAC1, Mutation of the OsSAC1 gene, which encodes an endoplasmic reticulum protein with an unknown function, causes sugar accumulation in rice leaves., Extremely more OsAGPL1 and OsAGPS1 (responsible for starch synthesis) expression and significantly less OscFBP1, OscFBP2, OsSPS1, and OsSPS11 (responsible for sucrose synthesis) and OsSWEET11, OsSWEET14, and OsSUT1 (responsible for sucrose loading) expression occurred in ossac1 source leaves than in WT source leaves
- Os8N3~xa13~OsSWEET11, OsDOF11, Rice Transcription Factor OsDOF11 Modulates Sugar Transport by Promoting Expression of Sucrose Transporter and SWEET genes., Chromatin immunoprecipitation experiments showed that OsDOF11 directly binds to the promoter regions of SUT1, OsSWEET11, and OsSWEET14, indicating that OsDOF11 coordinately controls transporters that are responsible for sucrose transport via apoplastic loading
- Os8N3~xa13~OsSWEET11, xa21, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, The present study was carried out to improve the cultivars (ASD 16 and ADT 43) through introgression of bacterial blight (xa5, xa13, and Xa21), blast (Pi54), and sheath blight (qSBR7-1, qSBR11-1, and qSBR11-2) resistance genes/QTLs by MABB (marker-assisted backcross breeding)
- Os8N3~xa13~OsSWEET11, xa21, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, IRBB60 (xa5, xa13, and Xa21) and Tetep (Pi54; qSBR7-1, qSBR11-1, and qSBR11-2) were used as donors to introgress BB, blast, and sheath blight resistance into the recurrent parents (ASD 16 and ADT 43)
- Os8N3~xa13~OsSWEET11, xa21, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, Homozygous (BC3F3 generation), three-gene bacterial blight pyramided (xa5 + xa13 + Xa21) lines were developed, and these lines were crossed with Tetep to combine blast (Pi54) and sheath blight (qSBR7-1, qSBR11-1, and qSBR11-2) resistance
- Os8N3~xa13~OsSWEET11, xa21, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, In BC3F3 generation, the improved pyramided lines carrying a total of seven genes/QTLs (xa5 + xa13 + Xa21 + Pi54 + qSBR7-1 + qSBR11-1 + qSBR11-2) were selected through molecular and phenotypic assay, and these were evaluated for resistance against bacterial blight, blast, and sheath blight pathogens under greenhouse conditions
- Os8N3~xa13~OsSWEET11, xa21, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, The present study successfully introgressed Pi54, and qSBR QTLs (qSBR7-1, qSBR11-1, and qSBR11-2) from Tetep and major effective BB-resistant genes (xa5, xa13, and Xa21) from IRBB60 into the commercial varieties for durable resistance to multiple diseases
- Os8N3~xa13~OsSWEET11, pikh~pi54~Pi-kh, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, The present study was carried out to improve the cultivars (ASD 16 and ADT 43) through introgression of bacterial blight (xa5, xa13, and Xa21), blast (Pi54), and sheath blight (qSBR7-1, qSBR11-1, and qSBR11-2) resistance genes/QTLs by MABB (marker-assisted backcross breeding)
- Os8N3~xa13~OsSWEET11, pikh~pi54~Pi-kh, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, Homozygous (BC3F3 generation), three-gene bacterial blight pyramided (xa5 + xa13 + Xa21) lines were developed, and these lines were crossed with Tetep to combine blast (Pi54) and sheath blight (qSBR7-1, qSBR11-1, and qSBR11-2) resistance
- Os8N3~xa13~OsSWEET11, pikh~pi54~Pi-kh, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, In BC3F3 generation, the improved pyramided lines carrying a total of seven genes/QTLs (xa5 + xa13 + Xa21 + Pi54 + qSBR7-1 + qSBR11-1 + qSBR11-2) were selected through molecular and phenotypic assay, and these were evaluated for resistance against bacterial blight, blast, and sheath blight pathogens under greenhouse conditions
- Os8N3~xa13~OsSWEET11, pikh~pi54~Pi-kh, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, The present study successfully introgressed Pi54, and qSBR QTLs (qSBR7-1, qSBR11-1, and qSBR11-2) from Tetep and major effective BB-resistant genes (xa5, xa13, and Xa21) from IRBB60 into the commercial varieties for durable resistance to multiple diseases
- Os8N3~xa13~OsSWEET11, Xa5, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, The present study was carried out to improve the cultivars (ASD 16 and ADT 43) through introgression of bacterial blight (xa5, xa13, and Xa21), blast (Pi54), and sheath blight (qSBR7-1, qSBR11-1, and qSBR11-2) resistance genes/QTLs by MABB (marker-assisted backcross breeding)
- Os8N3~xa13~OsSWEET11, Xa5, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, IRBB60 (xa5, xa13, and Xa21) and Tetep (Pi54; qSBR7-1, qSBR11-1, and qSBR11-2) were used as donors to introgress BB, blast, and sheath blight resistance into the recurrent parents (ASD 16 and ADT 43)
- Os8N3~xa13~OsSWEET11, Xa5, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, Homozygous (BC3F3 generation), three-gene bacterial blight pyramided (xa5 + xa13 + Xa21) lines were developed, and these lines were crossed with Tetep to combine blast (Pi54) and sheath blight (qSBR7-1, qSBR11-1, and qSBR11-2) resistance
- Os8N3~xa13~OsSWEET11, Xa5, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, In BC3F3 generation, the improved pyramided lines carrying a total of seven genes/QTLs (xa5 + xa13 + Xa21 + Pi54 + qSBR7-1 + qSBR11-1 + qSBR11-2) were selected through molecular and phenotypic assay, and these were evaluated for resistance against bacterial blight, blast, and sheath blight pathogens under greenhouse conditions
- Os8N3~xa13~OsSWEET11, Xa5, Gene Pyramiding for Achieving Enhanced Resistance to Bacterial Blight, Blast, and Sheath Blight Diseases in Rice, The present study successfully introgressed Pi54, and qSBR QTLs (qSBR7-1, qSBR11-1, and qSBR11-2) from Tetep and major effective BB-resistant genes (xa5, xa13, and Xa21) from IRBB60 into the commercial varieties for durable resistance to multiple diseases
- Os11N3~OsSWEET14, Os8N3~xa13~OsSWEET11, OsSWEET14 cooperates with OsSWEET11 to contribute to grain filling in rice, OsSWEET14 cooperates with OsSWEET11 to contribute to grain filling in rice
- Os11N3~OsSWEET14, Os8N3~xa13~OsSWEET11, OsSWEET14 cooperates with OsSWEET11 to contribute to grain filling in rice, ossweet14 knockout mutants did not show any detectable phenotypic differences from the wild type, whereas ossweet14;ossweet11 double-knockout mutants had much more severe phenotypes than ossweet11 single-knockout mutants, including strongly reduced grain weight and yield, reduced grain-filling rate, and increased starch accumulation in the pericarp
- Os11N3~OsSWEET14, Os8N3~xa13~OsSWEET11, OsSWEET14 cooperates with OsSWEET11 to contribute to grain filling in rice, Both OsSWEET14 and OsSWEET11 exhibited distinct spatiotemporal expression patterns between the early stage of caryopsis development and the rapid grain-filling stage
- Os11N3~OsSWEET14, Os8N3~xa13~OsSWEET11, OsSWEET14 cooperates with OsSWEET11 to contribute to grain filling in rice, During the rapid grain-filling stage, OsSWEET14 and OsSWEET11 localized to four key sites: vascular parenchyma cells, the nucellar projection, the nucellar epidermis, and cross cells
- Os8N3~xa13~OsSWEET11, OsDOF11, OsDOF11 Affects Nitrogen Metabolism by Sucrose Transport Signaling in Rice Oryza sativa L.., Previously, we noted that Oryza sativa DNA BINDING WITH ONE FINGER 11 (OsDOF11) mediated sucrose transport by binding to the promoter regions of Sucrose Transporter 1 (SUT1), Oryza sativa Sugars Will Eventually be Exported Transporters 11 (OsSWEET11), and OsSWEET14
- Os8N3~xa13~OsSWEET11, OsSWEET15, Knockout of OsSWEET15 Impairs Rice Embryo Formation and Seed-Setting., Investigation of ossweet11 mutant grains revealed similar phenotypes to those observed in the ossweet15 mutants
- Os8N3~xa13~OsSWEET11, OsSWEET15, Knockout of OsSWEET15 Impairs Rice Embryo Formation and Seed-Setting., These results indicate that both OsSWEET15 and OsSWEET11 play important and similar roles in rice pollen development, caryopsis formation and seed-setting, in addition to their function in seed-filling that was demonstrated previously
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