- Information
- Symbol: OsCPK21
- MSU: LOC_Os08g42750
- RAPdb: Os08g0540400
- Publication
- Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice, 2011, Plant Mol Biol.
- Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., 2017, Biochem Biophys Res Commun.
- OsCPK21 is required for pollen late-stage development in rice., 2019, J Plant Physiol.
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Genbank accession number
- Key message
- These results suggest that OsCPK21 is involved in the positive regulation of the signaling pathways that are involved in the response to ABA and salt stress
- Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice
- The survival rate of the OsCPK21-FOX plants was higher than that of wild-type (WT) plants grown under high salinity conditions
- Several ABA- and high salinity-inducible genes were more highly expressed in the OsCPK21-FOX plants than in WT plants
- The inhibition of seedling growth by abscisic acid (ABA) treatment was greater in the OsCPK21-FOX plants than in WT plants
- These results suggest that OsCPK21 phosphorylates OsGF14e to facilitate the response to ABA and salt stress
- We used an in<U+00A0>vitro kinase assay and site-directed mutagenesis to verify that OsCPK21 phosphorylates OsGF14e at Tyr-138
- Microarray analysis and qRT-PCR revealed that the transcription of OsCPK21 is coordinated with that of MIKC*-type MADS box transcription factors OsMADS62, OsMADS63, and OsMADS68 during rice anther development
- OsCPK21 is required for pollen late-stage development in rice.
- Here, we found that the knockdown of spikelet-specific OsCPK21 causes pollen abortion in OsCPK21-RNAi transgenic plants
- Connection
- OsCPK21, OsGF14e~GF14e~GID2, Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., In the present study, we performed yeast two-hybrid screening, glutathione S-transferase pull-down, co-immunoprecipitation, and bimolecular fluorescence complementation assays to confirm the interaction between OsCPK21 and one of its putative targets, Os14-3-3 (OsGF14e)
- OsCPK21, OsGF14e~GF14e~GID2, Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., We used an in<U+00A0>vitro kinase assay and site-directed mutagenesis to verify that OsCPK21 phosphorylates OsGF14e at Tyr-138
- OsCPK21, OsGF14e~GF14e~GID2, Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., These results suggest that OsCPK21 phosphorylates OsGF14e to facilitate the response to ABA and salt stress
- OsCPK21, OsHSD2, OsHSD2 interaction with and phosphorylation by OsCPK21 is essential for lipid metabolism during rice caryopsis development., OsHSD2 interaction with and phosphorylation by OsCPK21 is essential for lipid metabolism during rice caryopsis development.
- OsCPK21, OsHSD2, OsHSD2 interaction with and phosphorylation by OsCPK21 is essential for lipid metabolism during rice caryopsis development., Then, we verified the interaction between OsCPK21 and OsHSD2 using yeast two-hybrid and bimolecular fluorescence assays and revealed the in vitro phosphorylation of OsHSD2 by OsCPK21
- OsCPK21, OsHSD2, OsHSD2 interaction with and phosphorylation by OsCPK21 is essential for lipid metabolism during rice caryopsis development., Furthermore, oscpk21 and oshsd2 mutants were generated by the CRISPR/Cas9 technique, and we found that the lipid profiles were drastically changed in both oscpk21 and oshsd2, implying that OsHSD2 phosphorylated by OsCPK21 regulates lipid abundance in caryopsis development, thereby providing a potential target for the genetic improvement of rice grain quality in future lipid-related breeding and biotechnology applications
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